Construct: ORF ccsbBroadEn_08508
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012910.1_s300c1, BRDN0000456530
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PI4K2B (55300)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55300 | PI4K2B | phosphatidylinositol 4-kina... | NM_018323.4 | 99.7% | 99.7% | 232T>C;321C>T;333G>A |
| 2 | human | 55300 | PI4K2B | phosphatidylinositol 4-kina... | XM_005248174.2 | 98.7% | 98.7% | (many diffs) |
| 3 | human | 55300 | PI4K2B | phosphatidylinositol 4-kina... | XM_005248175.4 | 79.9% | 80% | 0_1ins288;33C>T;45G>A |
| 4 | human | 55300 | PI4K2B | phosphatidylinositol 4-kina... | NR_144633.2 | 39.9% | (many diffs) | |
| 5 | mouse | 67073 | Pi4k2b | phosphatidylinositol 4-kina... | XM_006504064.2 | 65.8% | 64.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1509
- ORF length:
- 1443
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAGAAAG 61 TTGGCATGGA GGATCCCTCC GAGCCCGACC GGTTGGCGTC CGCGGACGGC GGGAGCCCGG 121 AGGAGGAGGA GGATGGGGAG CGGGAGCCGC TGCTACCGCG GATCGCCTGG GCCCACCCGC 181 GGAGAGGCGC CCCAGGCAGC GCCGTGAGGC TGCTGGACGC TGCCGGGGAG GAGGGCGAGG 241 CCGGCGACGA GGAGCTGCCC CTCCCGCCCG GGGACGTGGG GGTCTCCCGG AGTTCGCCCG 301 CCGAGCTGGA CCGGAGCCGC CCCGCGGTTT CAGTAACTAT TGGTACTTCA GAGATGAATG 361 CATTCTTGGA TGACCCAGAA TTTGCTGATA TTATGCTAAG AGCAGAGCAA GCAATAGAAG 421 TTGGAATTTT TCCAGAAAGA ATCTCTCAAG GTTCAAGTGG AAGTTACTTT GTGAAGGATC 481 CTAAGAGGAA AATTATTGGT GTGTTTAAAC CCAAATCAGA AGAGCCTTAT GGTCAACTCA 541 ATCCAAAATG GACCAAATAT GTCCATAAGG TCTGCTGCCC TTGCTGCTTT GGCCGAGGCT 601 GCCTGATTCC TAATCAGGGG TACCTTTCCG AAGCGGGTGC CTATCTTGTG GACAACAAGC 661 TTCATCTGAG CATTGTACCT AAAACAAAGG TGGTTTGGCT TGTCAGTGAG ACATTTAACT 721 ATAATGCGAT TGACCGTGCA AAATCAAGAG GCAAAAAGTA TGCTTTAGAA AAAGTGCCAA 781 AAGTGGGTAG AAAGTTTCAT AGGATAGGAC TCCCTCCTAA GATTGGTTCC TTTCAGTTAT 841 TTGTTGAAGG TTACAAGGAG GCTGAATATT GGCTTAGGAA ATTTGAAGCT GACCCTTTGC 901 CTGAGAATAT TAGAAAACAA TTTCAGTCAC AATTTGAAAG ATTAGTTATT TTGGATTACA 961 TCATCAGAAA TACAGACAGG GGCAATGATA ATTGGTTAGT CAGATACGAA AAGCAGAAAT 1021 GTGAAAAGGA AATTGACCAT AAGGAATCAA AATGGATTGA TGATGAAGAA TTCCTTATTA 1081 AAATAGCTGC AATTGATAAT GGTCTAGCAT TTCCTTTTAA ACATCCTGAT GAATGGAGAG 1141 CATATCCATT TCACTGGGCT TGGCTTCCTC AAGCAAAAGT TCCCTTTTCT GAAGAAATAA 1201 GAAATTTGAT TCTACCATAT ATTTCTGACA TGAACTTTGT GCAAGATTTA TGTGAAGATC 1261 TCTATGAACT TTTTAAGACT GACAAAGGAT TTGACAAAGC CACTTTTGAA AGTCAGATGT 1321 CTGTGATGAG GGGTCAGATC TTAAACCTTA CTCAGGCATT GAGAGACGGG AAGAGTCCTT 1381 TCCAGCTAGT ACAGATACCT TGTGTGATTG TGGAACGCAG TCAAGGTGGA AGTCAGGGTC 1441 GGATTGTCCA CCTGAGCAAT TCCTTTACCC AGACTGTCAA TTGCAGGAAG CCATTTTTTT 1501 CCTCCTGGTA CCCAACTTTC TTGTACAAAG TTGGcattat aagaaagcat tgcttatcaa 1561 tttgttgcaa cgaac