Construct: ORF ccsbBroadEn_08566

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF015259.1_s300c1, BRDN0000389652
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
NSUN5 (55695)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 55695 NSUN5 NOP2/Sun RNA methyltransfer... NM_148956.4 100% 100%
2 human 55695 NSUN5 NOP2/Sun RNA methyltransfer... NM_001168347.3 98.4% 91.4% 1284_1285insAGCTC;1394_1410del
3 human 55695 NSUN5 NOP2/Sun RNA methyltransfer... NM_018044.4 91.9% 91.8% 1287_1287delGins112
4 human 55695 NSUN5 NOP2/Sun RNA methyltransfer... XR_242255.5 85.5% 1_36del;790_791insGGAA;1431_1626del
5 human 55695 NSUN5 NOP2/Sun RNA methyltransfer... NM_001168348.2 83.8% 83.6% 212_213ins114;1173_1173delGins112
6 human 55695 NSUN5 NOP2/Sun RNA methyltransfer... XM_005277604.5 81.7% 60.7% 754_755insGGAA;1143_1144ins251
7 human 155400 NSUN5P1 NSUN5 pseudogene 1 NR_033322.3 73.8% (many diffs)
8 human 260294 NSUN5P2 NSUN5 pseudogene 2 NR_033323.3 73.8% (many diffs)
9 human 155400 NSUN5P1 NSUN5 pseudogene 1 NR_104013.1 56.5% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1464
ORF length:
1398
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGGG GCTGTATGCT GCAGCTGCAG GCGTGTTGGC CGGCGTGGAG AGCCGCCAGG
121GCTCTATCAA GGGGTTGGTG TACTCCAGCA ACTTCCAGAA CGTGAAGCAG CTGTACGCGC
181TGGTGTGCGA AACGCAGCGC TACTCCGCCG TGCTGGATGC TGTGATCGCC AGCGCCGGCC
241TCCTCCGTGC GGAGAAGAAG CTGCGGCCGC ACCTGGCCAA GGTGCTAGTG TATGAGTTGT
301TGTTGGGAAA GGGCTTTCGA GGGGGTGGGG GCCGATGGAA GGCTCTGTTG GGCCGGCACC
361AGGCGAGGCT CAAGGCTGAG TTGGCTCGGC TCAAGGTTCA TCGGGGTGTG AGCCGGAATG
421AGGACCTGTT GGAAGTGGGA TCCAGGCCTG GTCCAGCCTC CCAGCTGCCT CGATTTGTGC
481GTGTGAACAC TCTCAAGACC TGCTCCGATG ATGTAGTTGA TTATTTCAAG AGACAAGGTT
541TCTCCTATCA GGGTCGGGCT TCCAGCCTCG ATGACTTACG AGCCCTCAAG GGGAAGCATT
601TTCTCCTGGA CCCCTTGATG CCGGAGCTGC TGGTGTTTCC CGCCCAGACA GATCTGCATG
661AACACCCACT GTACCGGGCC GGACACCTCA TTCTGCAGGA CAGGGCCAGC TGTCTCCCAG
721CCATGCTGCT GGACCCCCCG CCAGGCTCCC ATGTCATCGA TGCCTGTGCC GCCCCAGGCA
781ATAAGACCAG TCACTTGGCT GCTCTTCTGA AGAACCAAGG GAAGATCTTT GCCTTTGACC
841TGGATGCCAA GCGGCTGGCA TCCATGGCCA CGCTGCTGGC CCGGGCTGGC GTCTCTTGCT
901GTGAACTGGC TGAGGAGGAC TTCCTGGCGG TCTCCCCCTC GGATCCACGC TACCATGAGG
961TCCACTACAT CCTGCTGGAT CCTTCCTGCA GTGGCTCGGG TATGCCGAGC AGACAGCTGG
1021AGGAGCCCGG GGCAGGCACA CCTAGCCCGG TGCGTCTGCA TGCCCTGGCA GGGTTCCAGC
1081AGCGAGCCCT GTGCCACGCA CTCACTTTCC CTTCCCTGCA GCGGCTCGTC TACTCCACGT
1141GCTCCCTCTG CCAGGAGGAG AATGAAGACG TGGTGCGAGA TGCGCTGCAG CAGAACCCGG
1201GCGCCTTCAG GCTAGCTCCC GCCCTGCCTG CCTGGCCCCA CCGAGGCCTG AGCACGTTCC
1261CGGGTGCCGA GCACTGCCTC CGGGCCTCCC CTGAGACCAC ACTCAGCAGT GGCTTCTTCG
1321TTGCTGTAAT TGAACGGGTC GAGGTGCCAA GCTCAGCCTC ACAGGCCAAA GCATCAGCAC
1381CAGAACGCAC ACCCAGCCCA GCCCCAAAGA GAAAGAAGAG ACAGCAAAGA GCCGCAGCCG
1441GTGCTTGCAC ACCGCCTTGC ACATACCCAA CTTTCTTGTA CAAAGTtggc attataagaa
1501agcattgctt atcaatttgt tgcaacgaac

Download FASTA (ORF) (Full)

GPP Web Portal Terms of Service

Effective Date: December 8, 2025
By using this site, you agree to our terms and conditions below.

Overview of Terms

The data made available on this website were generated for research purposes and are not intended for clinical or commercial uses. Commercial use (or other use for profit-making purposes) of the GPP Web Portal and its tools, is not permitted under these terms and may require a separate license agreement from Broad or its contributors. For more information, please contact partnering@broadinstitute.org.

The original data may be subject to rights claimed by third parties, including but not limited to, patent, copyright, other intellectual property rights, biodiversity-related access and benefit-sharing rights. It is the responsibility of users of Broad Institute services to ensure that their use of the data does not infringe any of the rights of such third parties.

Any questions or comments concerning these Terms of Use can be addressed to: legal@broadinstitute.org.

By accessing and viewing this GPP Web Portal, you agree to the following terms and conditions:

Attribution

You agree to acknowledge the Broad Institute (e.g., in publications, services or products) for any of your use of its online services, databases or software in accordance with good scientific practice. You agree to use the acknowledgment wording provided for the relevant tools as indicated on the FAQ for each tool.

Updating the Terms of Use

We reserve the right to update these Terms of Use at any time. When alterations are inevitable, we will attempt to give reasonable notice of any changes by placing a notice on our website, but you may wish to check each time you use the website. The date of the most recent revision will appear on this, the "GPP Web Portal Terms of Use" page. If you do not agree to these changes, please do not continue to use our online services. We will also make available an archived copy of the previous Terms of Use for comparison.

Indemnification and Disclaimer of Warranties

You are using this GPP Web Portal at your own risk, and you hereby agree to hold Broad and its contributors and their trustees, directors, officers, employees, and affiliated investigators harmless for any third party claims which may arise from your use of the GPP Web Portal, the tools available therein, or any portion thereof. Further, you agree to indemnify Broad, its contributors, and its and their trustees, directors, officers, employees, affiliated investigators, students, and affiliates for any loss, costs, claims, damages, or other liabilities arising from any unpermitted commercial or profit-making use you make of the GPP Web Portal. The GPP Web Portal is a research tool and is provided "as is". Broad does not represent that the GPP Web Portal is free of errors or bugs or suitable for any particular tasks.

ANY EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT LIMITED TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE, NONINFRINGEMENT, OR THE ABSENCE OF LATENT OR OTHER DEFECTS ARE DISCLAIMED. IN NO EVENT SHALL BROAD OR ITS CONTRIBUTORS BE LIABLE FOR ANY DIRECT, INDIRECT, INCIDENTAL, SPECIAL, EXEMPLARY, OR CONSEQUENTIAL DAMAGES HOWEVER CAUSED AND ON ANY THEORY OF LIABILITY, WHETHER IN CONTRACT, STRICT LIABILITY, OR TORT (INCLUDING NEGLIGENCE OR OTHERWISE) ARISING IN ANY WAY OUT OF THE USE OF THE GPP WEB PORTAL, EVEN IF ADVISED OF THE POSSIBILITY OF SUCH DAMAGE.

Governing Law

The terms and conditions herein shall be construed, governed, interpreted, and applied in accordance with the internal laws of the Commonwealth of Massachusetts, U.S.A. Furthermore, by accessing, downloading, or using the Database, You consent to the personal jurisdiction of, and venue in, the state and federal courts within Massachusetts with respect to Your download or use of the Database.