Construct: ORF ccsbBroadEn_08572

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF007393.1_s300c1, BRDN0000398365
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
HHAT (55733)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 55733 HHAT hedgehog acyltransferase NM_001122834.3 99.8% 99.7% 534G>C;545G>A
2 human 55733 HHAT hedgehog acyltransferase NM_001170580.3 99.8% 99.7% 534G>C;545G>A
3 human 55733 HHAT hedgehog acyltransferase NM_018194.6 99.8% 99.7% 534G>C;545G>A
4 human 55733 HHAT hedgehog acyltransferase NM_001170587.2 93.9% 90.6% (many diffs)
5 human 55733 HHAT hedgehog acyltransferase XM_006711441.3 93.4% 93.3% 1_102del;636G>C;647G>A
6 human 55733 HHAT hedgehog acyltransferase XM_011509739.2 92% 88% (many diffs)
7 human 55733 HHAT hedgehog acyltransferase XM_011509740.2 92% 88% (many diffs)
8 human 55733 HHAT hedgehog acyltransferase XM_011509741.2 92% 88% (many diffs)
9 human 55733 HHAT hedgehog acyltransferase XM_017001729.1 92% 88% (many diffs)
10 human 55733 HHAT hedgehog acyltransferase XM_017001734.1 90.7% 89.2% (many diffs)
11 human 55733 HHAT hedgehog acyltransferase XM_017001728.1 87.7% 83.9% (many diffs)
12 human 55733 HHAT hedgehog acyltransferase XM_011509747.1 87% 87% 0_1ins189;345G>C;356G>A
13 human 55733 HHAT hedgehog acyltransferase XM_017001736.1 87% 87% 0_1ins189;345G>C;356G>A
14 human 55733 HHAT hedgehog acyltransferase NM_001170588.2 86.6% 86.6% 270_271ins195;339G>C;350G>A
15 human 55733 HHAT hedgehog acyltransferase XM_017001732.1 86.6% 86.6% 270_271ins195;339G>C;350G>A
16 human 55733 HHAT hedgehog acyltransferase XM_017001735.1 86.6% 86.6% 270_271ins195;339G>C;350G>A
17 human 55733 HHAT hedgehog acyltransferase XM_011509738.2 86.6% 79.9% (many diffs)
18 human 55733 HHAT hedgehog acyltransferase XM_011509737.2 86.4% 82.7% (many diffs)
19 human 55733 HHAT hedgehog acyltransferase XM_017001730.1 83.4% 78.2% (many diffs)
20 human 55733 HHAT hedgehog acyltransferase XM_017001731.1 83.4% 78.2% (many diffs)
21 human 55733 HHAT hedgehog acyltransferase XM_011509744.2 82.9% 78.4% (many diffs)
22 human 55733 HHAT hedgehog acyltransferase XM_017001733.1 80% 76.1% (many diffs)
23 human 55733 HHAT hedgehog acyltransferase XM_011509746.2 79.6% 75.7% (many diffs)
24 human 55733 HHAT hedgehog acyltransferase XM_011509742.2 74.8% 71.1% (many diffs)
25 human 55733 HHAT hedgehog acyltransferase XM_017001726.1 73.9% 68.4% (many diffs)
26 human 55733 HHAT hedgehog acyltransferase NM_001170564.2 72.2% 72.2% 271_272ins411
27 human 55733 HHAT hedgehog acyltransferase XM_017001737.1 72.2% 72.2% 271_272ins411
28 human 55733 HHAT hedgehog acyltransferase XM_017001738.1 66% 62.3% (many diffs)
29 human 55733 HHAT hedgehog acyltransferase XM_017001739.1 56.4% 36.4% (many diffs)
30 human 55733 HHAT hedgehog acyltransferase XM_017001740.1 56.4% 36.4% (many diffs)
31 human 55733 HHAT hedgehog acyltransferase XM_017001741.1 56.4% 36.4% (many diffs)
32 human 55733 HHAT hedgehog acyltransferase XM_017001742.1 56.4% 36.4% (many diffs)
33 human 55733 HHAT hedgehog acyltransferase XM_017001743.1 56.4% 36.4% (many diffs)
34 human 55733 HHAT hedgehog acyltransferase XM_017001744.1 56.4% 36.4% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1548
ORF length:
1479
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaacttTG TACAAAAAAG
61TTGGCACCAT GCTGCCCCGA TGGGAACTGG CACTTTACCT ACTTGCCTCA CTAGGCTTCC
121ACTTCTATTC CTTCTATGAA GTTTACAAAG TCTCCAGAGA ACACGAAGAG GAGCTGGACC
181AGGAATTTGA GCTGGAGACT GACACTTTAT TTGGAGGATT AAAGAAGGAT GCGACCGACT
241TTGAGTGGAG CTTCTGGATG GAATGGGGGA AGCAGTGGCT GGTGTGGCTT CTCCTTGGCC
301ACATGGTAGT GTCTCAAATG GCCACACTGC TGGCAAGAAA GCACAGACCC TGGATTCTCA
361TGCTCTATGG GATGTGGGCC TGCTGGTGTG TGCTGGGGAC CCCTGGTGTG GCTATGGTTT
421TGCTCCATAC CACCATCTCT TTCTGCGTGG CCCAGTTCCG GTCTCAGCTC CTGACGTGGC
481TCTGTTCTCT CCTCCTCCTC TCCACACTGA GGCTGCAGGG TGTGGAAGAA GTTAAGAGAA
541GGTGGTACAA GACAGAAAAC GAGTACTACC TGCTGCAGTT CACGCTGACC GTTCGCTGCC
601TCTACTACAC CAACTTCAGC CTGGAGCTCT GCTGGCAGCA GCTGCCTGCT GCATCGACCT
661CCTACTCCTT TCCCTGGATG CTGGCCTATG TCTTTTATTA TCCAGTCTTA CACAATGGGC
721CCATCCTCAG CTTCTCGGAG TTCATCAAAC AGATGCAGCA GCAGGAGCAT GACTCCCTGA
781AGGCCAGCCT GTGTGTCCTG GCCCTGGGGC TGGGCCGCCT TCTTTGCTGG TGGTGGCTGG
841CCGAGCTGAT GGCTCACCTG ATGTACATGC ATGCCATCTA CAGCAGCATC CCCCTCCTGG
901AGACTGTCTC TTGTTGGACC TTAGGAGGAC TGGCGTTAGC CCAGGTGCTC TTTTTCTACG
961TGAAGTACTT GGTGCTCTTT GGCGTGCCTG CTCTGCTCAT GCGCCTGGAT GGACTCACTC
1021CACCCGCCCT CCCCCGCTGC GTGAGCACCA TGTTCAGTTT CACCGGGATG TGGAGGTATT
1081TTGATGTTGG ACTGCATAAT TTCTTAATCA GGTATGTGTA CATTCCAGTG GGCGGGTCCC
1141AGCATGGCCT GCTGGGGACA CTGTTTTCCA CGGCGATGAC ATTTGCATTT GTGAGCTACT
1201GGCATGGCGG CTACGACTAC CTCTGGTGCT GGGCAGCGCT CAACTGGCTG GGAGTCACTG
1261TGGAGAATGG AGTCCGGAGG CTGGTGGAGA CTCCCTGCAT CCAGGACAGT CTGGCCCGAT
1321ACTTCTCCCC ACAAGCTCGC CGTCGATTCC ACGCTGCCCT TGCTTCTTGT TCCACCTCGA
1381TGCTGATCCT GTCCAACCTG GTATTTCTTG GGGGCAATGA GGTTGGGAAA ACCTACTGGA
1441ATAGGATCTT CATACAAGGC TGGCCTTGGG TGACCCTCTC TGTCCTGGGA TTCCTGTACT
1501GCTACTCCCA CGTGGGCATT GCCTGGGCCC AGACCTACGC CACGGACTTG CCAACTTTCT
1561TGTACAAAGT tggcattata agaaagcatt gcttatcaat ttgttgcaac gaac

Download FASTA (ORF) (Full)