Construct: ORF ccsbBroadEn_08583

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF006613.1_s300c1, BRDN0000391096
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
IFT122 (55764)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 55764 IFT122 intraflagellar transport 122 NM_052990.3 99.9% 100% 1650A>T
2 human 55764 IFT122 intraflagellar transport 122 XM_017006829.2 99.8% 99.9% 1650A>T;2456_2457insAGC
3 human 55764 IFT122 intraflagellar transport 122 XM_017006833.2 98.3% 98.4% 1650A>T;1657_1658ins54
4 human 55764 IFT122 intraflagellar transport 122 XM_017006834.2 98.2% 98.3% 1650A>T;1657_1658ins54;2402_2403insAGC
5 human 55764 IFT122 intraflagellar transport 122 XM_017006828.2 96.4% 95.6% (many diffs)
6 human 55764 IFT122 intraflagellar transport 122 XM_005247609.2 95.5% 95.6% 192_347del;1806A>T
7 human 55764 IFT122 intraflagellar transport 122 NM_018262.4 95.4% 95.5% 192_347del;1806A>T;2612_2613insAGC
8 human 55764 IFT122 intraflagellar transport 122 XM_006713692.3 94% 94% 192_347del;1806A>T;1813_1814ins54
9 human 55764 IFT122 intraflagellar transport 122 XM_017006827.2 93.9% 93.9% (many diffs)
10 human 55764 IFT122 intraflagellar transport 122 XM_017006823.2 92.3% 91.5% (many diffs)
11 human 55764 IFT122 intraflagellar transport 122 XM_017006824.2 92.2% 91.4% (many diffs)
12 human 55764 IFT122 intraflagellar transport 122 XM_017006822.2 91.8% 90.9% (many diffs)
13 human 55764 IFT122 intraflagellar transport 122 NM_001280541.2 91.6% 91.6% 192_500del;1959A>T
14 human 55764 IFT122 intraflagellar transport 122 NM_001280546.1 91.2% 91.2% 0_1ins294;1356A>T;2162_2163insAGC
15 human 55764 IFT122 intraflagellar transport 122 XM_006713689.2 91% 90.9% 192_347del;564_740del;1983A>T
16 human 55764 IFT122 intraflagellar transport 122 NM_052989.3 90.9% 90.9% (many diffs)
17 human 55764 IFT122 intraflagellar transport 122 XM_017006825.2 90.8% 90% (many diffs)
18 human 55764 IFT122 intraflagellar transport 122 XM_017006826.2 90.7% 89.9% (many diffs)
19 human 55764 IFT122 intraflagellar transport 122 XM_017006836.2 89.7% 89.7% 0_1ins294;1356A>T;1363_1364ins54
20 human 55764 IFT122 intraflagellar transport 122 XM_006713691.3 89.5% 89.5% (many diffs)
21 human 55764 IFT122 intraflagellar transport 122 XM_017006821.2 89.5% 89.4% (many diffs)
22 human 55764 IFT122 intraflagellar transport 122 XM_017006817.2 88% 87.1% (many diffs)
23 human 55764 IFT122 intraflagellar transport 122 XM_017006835.1 88% 87.1% (many diffs)
24 human 55764 IFT122 intraflagellar transport 122 XM_017006818.2 88% 87.1% (many diffs)
25 human 55764 IFT122 intraflagellar transport 122 NM_052985.4 87.3% 87.3% (many diffs)
26 human 55764 IFT122 intraflagellar transport 122 NM_001280545.1 86.6% 86.6% (many diffs)
27 human 55764 IFT122 intraflagellar transport 122 XM_017006819.2 86.6% 85.7% (many diffs)
28 human 55764 IFT122 intraflagellar transport 122 XM_017006820.2 86.5% 85.7% (many diffs)
29 human 55764 IFT122 intraflagellar transport 122 XM_017006830.2 83.8% 82.8% (many diffs)
30 human 55764 IFT122 intraflagellar transport 122 XM_017006831.1 83.8% 82.8% (many diffs)
31 human 55764 IFT122 intraflagellar transport 122 XM_017006832.1 83.8% 82.8% (many diffs)
32 human 55764 IFT122 intraflagellar transport 122 XM_024453639.1 81.2% 80.1% (many diffs)
33 human 55764 IFT122 intraflagellar transport 122 XM_017006838.2 68.3% 68.3% 0_1ins1071;579A>T;1385_1386insAGC
34 human 55764 IFT122 intraflagellar transport 122 XM_011512972.3 68.1% 65.9% (many diffs)
35 human 55764 IFT122 intraflagellar transport 122 XM_017006837.1 65.7% 64.8% (many diffs)
36 human 55764 IFT122 intraflagellar transport 122 XM_006713695.3 60.8% 59.2% (many diffs)
37 mouse 81896 Ift122 intraflagellar transport 122 NM_031177.4 84.1% 87.4% (many diffs)
38 mouse 81896 Ift122 intraflagellar transport 122 NM_001167763.1 84% 87.3% (many diffs)
39 mouse 81896 Ift122 intraflagellar transport 122 XM_006506797.2 80.1% 83.1% (many diffs)
40 mouse 81896 Ift122 intraflagellar transport 122 XM_006506798.2 80% 83% (many diffs)
41 mouse 81896 Ift122 intraflagellar transport 122 XM_006506799.3 76.1% 78.9% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
3459
ORF length:
3393
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGAG GGCCGTGTTG ACGTGGAGAG ATAAAGCCGA GCACTGTATA AATGACATCG
121CATTTAAGCC TGATGGAACT CAACTGATTT TGGCTGCCGG AAGCAGATTA CTGGTTTATG
181ACACCTCTGA TGGCACCTTA CTTCAGCCCC TCAAGGGACA CAAAGACACT GTGTACTGTG
241TGGCATATGC GAAGGATGGG TTGTGGTCTC CTGAACAGAA GTCTGTCTCC AAACACAAAT
301CAAGCAGCAA GATCATCTGC TGCAGCTGGA CAAATGATGG TCAGTACCTG GCGCTGGGGA
361TGTTCAATGG GATCATCAGC ATACGGAACA AAAATGGCGA GGAGAAAGTA AAGATCGAGC
421GGCCGGGGGG CTCCCTCTCG CCAATATGGT CCATCTGCTG GAACCCTTCA AGAGAGGAAC
481GTAATGACAT CCTGGCTGTG GCTGACTGGG GACAGAAAGT TTCCTTCTAC CAGCTGAGTG
541GAAAACAGAT TGGAAAGGAT CGGGCACTGA ACTTTGACCC CTGCTGCATC AGCTACTTTA
601CTAAAGGCGA GTACATTTTG CTGGGGGGTT CAGACAAGCA AGTATCTCTT TTCACCAAGG
661ATGGAGTGCG GCTTGGGACT GTTGGGGAGC AGAACTCCTG GGTGTGGACG TGTCAAGCGA
721AACCGGATTC CAACTATGTG GTGGTCGGCT GCCAGGACGG CACCATTTCC TTCTACCAGC
781TTATTTTCAG CACAGTCCAT GGGCTTTACA AGGACCGCTA TGCCTACAGG GATAGCATGA
841CTGACGTCAT TGTGCAGCAC CTGATCACTG AGCAGAAAGT TCGGATTAAA TGCAAAGAGC
901TTGTCAAGAA GATTGCCATC TACAGAAATC GATTGGCTAT CCAACTGCCA GAGAAAATCC
961TCATCTATGA GTTGTATTCA GAGGACTTAT CAGACATGCA TTACCGGGTA AAGGAGAAGA
1021TTATCAAGAA GTTTGAGTGC AACCTCCTGG TGGTGTGTGC CAATCACATC ATCCTGTGCC
1081AGGAGAAACG GCTGCAGTGC CTGTCCTTCA GCGGAGTGAA GGAGCGGGAG TGGCAGATGG
1141AGTCTCTCAT TCGTTACATC AAGGTGATCG GTGGCCCTCC TGGAAGAGAA GGCCTCTTAG
1201TGGGGCTGAA GAATGGACAG ATCCTGAAGA TCTTCGTGGA CAATCTCTTT GCTATCGTCC
1261TGCTGAAGCA GGCCACAGCT GTGCGCTGCT TGGACATGAG TGCCTCCCGT AAGAAGCTGG
1321CCGTGGTAGA TGAAAATGAC ACTTGCCTGG TGTATGACAT CGACACCAAG GAGCTGCTTT
1381TTCAGGAACC AAACGCCAAC AGTGTAGCTT GGAACACCCA GTGTGAGGAC ATGCTCTGCT
1441TCTCGGGAGG AGGCTACCTC AACATCAAAG CCAGCACCTT CCCTGTGCAC CGGCAGAAGC
1501TGCAGGGCTT TGTGGTCGGC TACAATGGCT CCAAGATCTT CTGCCTCCAT GTCTTCTCCA
1561TTTCTGCCGT GGAGGTGCCG CAGTCCGCTC CCATGTACCA GTACCTGGAT AGGAAACTGT
1621TCAAGGAAGC CTACCAGATT GCTTGCTTGG GTGTCACAGA CACTGATTGG CGTGAACTGG
1681CCATGGAAGC GCTAGAAGGT TTAGATTTTG AAACTGCAAA GAAGGCCTTC ATCAGAGTAC
1741AAGACCTCCG ATATTTAGAG CTCATCAGCA GCATTGAGGA GAGGAAGAAG CGGGGAGAGA
1801CCAACAATGA CCTGTTTCTG GCAGATGTGT TTTCCTACCA GGGGAAGTTC CATGAGGCCG
1861CCAAACTGTA CAAGAGGAGT GGGCACGAGA ACCTCGCGCT TGAAATGTAC ACCGACCTCT
1921GCATGTTTGA GTATGCCAAG GATTTCCTTG GATCTGGAGA CCCCAAAGAA ACAAAGATGC
1981TAATCACCAA ACAGGCTGAC TGGGCCAGAA ATATCAAGGA GCCCAAAGCC GCCGTGGAGA
2041TGTACATCTC AGCAGGAGAG CACGTCAAGG CCATCGAGAT CTGTGGTGAC CATGGCTGGG
2101TTGACATGTT GATCGACATC GCCCGCAAAC TGGACAAGGC TGAGCGCGAG CCCCTGCTGC
2161TGTGCGCTAC CTACCTCAAG AAGCTGGACA GCCCTGGCTA TGCTGCTGAG ACCTACCTGA
2221AGATGGGTGA CCTCAAGTCC CTGGTGCAGC TGCACGTGGA GACCCAGCGC TGGGATGAGG
2281CCTTTGCTTT GGGTGAGAAG CATCCTGAGT TTAAGGATGA CATCTACATG CCGTATGCTC
2341AGTGGCTAGC AGAGAACGAT CGCTTTGAGG AAGCCCAGAA AGCGTTCCAC AAGGCTGGGC
2401GACAGAGAGA AGCGGTCCAG GTGCTGGAGC AGCTCACAAA CAATGCCGTG GCGGAGAGCA
2461GGTTTAATGA TGCTGCCTAT TATTACTGGA TGCTGTCCAT GCAGTGCCTC GATATAGCTC
2521AAGCAGATCC TGCCCAGAAG GACACAATGC TTGGCAAGTT CTACCACTTC CAGCGTTTGG
2581CAGAGCTGTA CCATGGTTAC CATGCCATCC ATCGCCACAC GGAAGATCCG TTCAGTGTCC
2641ATCGTCCTGA AACTCTTTTC AACATCTCCA GGTTCCTGCT GCACAGCCTG CCCAAGGACA
2701CCCCCTCGGG CATCTCTAAA GTGAAAATAC TCTTCACCTT GGCCAAGCAG AGCAAGGCCC
2761TCGGTGCCTA CAGGCTGGCC CGGCACGCCT ATGACAAGCT GCGTGGCCTG TACATCCCTG
2821CCAGATTCCA AAAGTCCATT GAGCTGGGTA CCCTGACCAT CCGCGCCAAG CCCTTCCACG
2881ACAGTGAGGA GTTGGTGCCC TTGTGCTACC GCTGCTCCAC CAACAACCCG CTGCTCAACA
2941ACCTGGGCAA CGTCTGCATC AACTGCCGCC AGCCCTTCAT CTTCTCCGCC TCTTCCTACG
3001ACGTGCTACA CCTGGTTGAG TTCTACCTGG AGGAAGGGAT CACTGATGAA GAAGCCATCT
3061CCCTCATCGA CCTGGAGGTG CTGAGACCCA AGCGGGATGA CAGACAGCTA GAGATTGCAA
3121ACAACAGCTC CCAGATTCTG CGGCTAGTGG AGACCAAGGA CTCCATCGGA GATGAGGACC
3181CGTTCACAGC TAAGCTGAGC TTTGAGCAAG GTGGCTCAGA GTTCGTGCCA GTGGTGGTGA
3241GCCGGCTGGT GCTGCGCTCC ATGAGCCGCC GGGATGTCCT CATCAAGCGA TGGCCCCCAC
3301CCCTGAGGTG GCAATACTTC CGCTCACTGC TGCCTGACGC CTCCATTACC ATGTGCCCCT
3361CCTGCTTCCA GATGTTCCAT TCTGAGGACT ATGAGTTGCT GGTGCTTCAG CATGGCTGCT
3421GCCCCTACTG CCGCAGGTGC AAGGATGACC CTGGCCCATG CCCAACTTTC TTGTACAAAG
3481Ttggcattat aagaaagcat tgcttatcaa tttgttgcaa cgaac

Download FASTA (ORF) (Full)