Transcript: Mouse NM_001167763.1

Mus musculus intraflagellar transport 122 (Ift122), transcript variant 2, mRNA.

Source:
NCBI, updated 2017-05-27
Taxon:
Mus musculus (mouse)
Gene:
Ift122 (81896)
Length:
4080
CDS:
142..3690

Additional Resources:

NCBI RefSeq record:
NM_001167763.1
NBCI Gene record:
Ift122 (81896)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_001167763.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000202308 GCCGCTGCAATGGCAATATTT pLKO.1 3528 CDS 100% 15.000 21.000 N Ift122 n/a
2 TRCN0000249610 GCCGCTGCAATGGCAATATTT pLKO_005 3528 CDS 100% 15.000 21.000 N Ift122 n/a
3 TRCN0000217390 GTTTAACGACGCTGCCTATTA pLKO.1 2694 CDS 100% 13.200 18.480 N Ift122 n/a
4 TRCN0000249609 TACACGCACAACGACTCTATA pLKO_005 409 CDS 100% 13.200 10.560 N Ift122 n/a
5 TRCN0000215635 GTGAAACCTGACTCCAATTAT pLKO.1 949 CDS 100% 15.000 10.500 N Ift122 n/a
6 TRCN0000249608 TAACCACAGATTTCCTATTTA pLKO_005 3832 3UTR 100% 15.000 10.500 N Ift122 n/a
7 TRCN0000257926 TAACGACGCTGCCTATTATTA pLKO_005 2697 CDS 100% 15.000 10.500 N Ift122 n/a
8 TRCN0000192074 CCCAGATGAAGTTTGTATGTT pLKO.1 3785 3UTR 100% 5.625 3.938 N Ift122 n/a
Download CSV

shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001167763.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

Download CSV

All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_08583 pDONR223 100% 84% 87.3% None (many diffs) n/a
2 ccsbBroad304_08583 pLX_304 0% 84% 87.3% V5 (many diffs) n/a
3 TRCN0000471866 TCCGCGTTGTTACTGGTCATCAGA pLX_317 6.3% 84% 87.3% V5 (many diffs) n/a
Download CSV