Construct: ORF ccsbBroadEn_08691
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000052.1_s300c1, BRDN0000388209
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TWSG1 (57045)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57045 | TWSG1 | twisted gastrulation BMP si... | NM_020648.6 | 99.8% | 99.5% | 21T>N |
| 2 | mouse | 65960 | Twsg1 | twisted gastrulation BMP si... | NM_023053.3 | 84.3% | 93.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 735
- ORF length:
- 669
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG 61 TTGGCATGAA GTTACACTAT GTTGCNGTGC TTACTCTAGC CATCCTGATG TTCCTGACAT 121 GGCTTCCAGA ATCACTGAGC TGTAACAAAG CACTCTGTGC TAGTGATGTG AGCAAATGCC 181 TCATTCAGGA GCTCTGCCAG TGCCGGCCGG GAGAAGGCAA TTGCTCCTGC TGTAAGGAGT 241 GCATGCTGTG TCTTGGGGCC CTTTGGGACG AGTGCTGTGA CTGTGTTGGT ATGTGTAATC 301 CTCGAAATTA TAGTGACACA CCTCCAACTT CAAAGAGCAC AGTGGAGGAG CTGCATGAAC 361 CGATCCCTTC TCTCTTCCGG GCACTCACAG AAGGAGATAC TCAGTTGAAT TGGAACATCG 421 TTTCTTTCCC TGTTGCAGAA GAACTTTCAC ATCATGAGAA TCTGGTTTCA TTTTTAGAAA 481 CTGTGAACCA GCCACACCAC CAGAATGTGT CTGTCCCCAG CAATAATGTT CACGCGCCTT 541 ATTCCAGTGA CAAAGAACAC ATGTGTACTG TGGTTTATTT TGATGACTGC ATGTCCATAC 601 ATCAGTGTAA AATATCCTGT GAGTCCATGG GAGCATCCAA ATATCGCTGG TTTCATAATG 661 CCTGCTGCGA GTGCATTGGT CCAGAATGTA TTGACTATGG TAGTAAAACT GTCAAATGTA 721 TGAACTGCAT GTTTTACCCA ACTTTCTTGT ACAAAGTtgg cattataaga aagcattgct 781 tatcaatttg ttgcaacgaa c