Construct: ORF ccsbBroadEn_08777
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007457.1_s300c1, BRDN0000388874
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PCTP (58488)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 58488 | PCTP | phosphatidylcholine transfe... | NM_021213.4 | 99.8% | 100% | 483C>T |
| 2 | human | 58488 | PCTP | phosphatidylcholine transfe... | NM_001330377.2 | 91% | 91.5% | (many diffs) |
| 3 | human | 58488 | PCTP | phosphatidylcholine transfe... | NM_001330378.2 | 90.4% | 84.9% | (many diffs) |
| 4 | human | 58488 | PCTP | phosphatidylcholine transfe... | NM_001102402.2 | 66.1% | 66.3% | 0_1ins216;267C>T |
| 5 | human | 58488 | PCTP | phosphatidylcholine transfe... | XM_017024913.1 | 57.9% | 57.9% | (many diffs) |
| 6 | human | 58488 | PCTP | phosphatidylcholine transfe... | XR_001752577.1 | 30.1% | (many diffs) | |
| 7 | human | 58488 | PCTP | phosphatidylcholine transfe... | XR_243677.4 | 20.1% | (many diffs) | |
| 8 | human | 58488 | PCTP | phosphatidylcholine transfe... | XR_001752576.1 | 18.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 708
- ORF length:
- 642
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG 61 TTGGCATGGA GCTGGCCGCC GGAAGCTTCT CGGAGGAGCA GTTCTGGGAG GCCTGCGCCG 121 AGCTCCAGCA GCCCGCTCTG GCCGGGGCCG ACTGGCAGCT CCTAGTGGAG ACCTCGGGCA 181 TCAGCATCTA CCGGCTGCTG GACAAGAAGA CTGGACTTTA TGAGTATAAA GTCTTTGGTG 241 TTCTGGAGGA CTGCTCACCA ACTCTACTGG CAGACATCTA TATGGACTCA GATTACAGAA 301 AACAATGGGA CCAGTATGTT AAAGAACTCT ATGAACAAGA ATGCAACGGA GAGACTGTGG 361 TCTACTGGGA AGTGAAGTAC CCTTTTCCCA TGTCCAACAG AGACTATGTC TACCTTCGGC 421 AGCGGCGAGA CCTGGACATG GAAGGGAGGA AGATCCATGT GATCCTGGCC CGGAGCACCT 481 CCATGCCTCA GCTTGGCGAG AGGTCTGGGG TGATCCGGGT GAAGCAATAC AAGCAGAGCC 541 TGGCGATTGA GAGTGACGGC AAGAAGGGGA GCAAAGTTTT CATGTATTAC TTCGATAACC 601 CGGGTGGCCA AATTCCGTCC TGGCTCATTA ACTGGGCCGC CAAGAATGGA GTTCCTAACT 661 TCTTGAAAGA CATGGCAAGA GCCTGTCAGA ACTACCTCAA GAAAACCTAC CCAACTTTCT 721 TGTACAAAGT tggcattata agaaagcatt gcttatcaat ttgttgcaac gaac