Construct: ORF ccsbBroadEn_08808

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF017256.1_s300c1, BRDN0000386655
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
MRPL17 (63875)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 63875 MRPL17 mitochondrial ribosomal pro... NM_022061.4 99.8% 99.4% 520G>C
2 mouse 27397 Mrpl17 mitochondrial ribosomal pro... NM_025301.2 84.6% 84% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
591
ORF length:
525
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGCG GCTGTCGGTC GCTGCAGCGA TCTCCCATGG CCGCGTATTT CGCCGTATGG
121GCCTCGGTCC CGAGTCCCGC ATCCATCTGT TGCGGAACTT GCTCACAGGG CTGGTGCGGC
181ACGAACGCAT CGAGGCACCA TGGGCGCGTG TGGACGAAAT GAGGGGCTAC GCGGAGAAGC
241TCATCGACTA TGGGAAGCTG GGAGACACTA ACGAACGAGC CATGCGCATG GCTGACTTCT
301GGCTCACAGA GAAGGATTTG ATCCCAAAGC TGTTTCAAGT ACTGGCCCCT CGGTACAAAG
361ATCAAACTGG GGGCTACACA AGAATGCTGC AGATCCCAAA TCGGAGTTTG GATCGGGCCA
421AGATGGCAGT GATCGAGTAT AAAGGGAATT GCCTCCCACC CCTGCCTCTG CCTCGCAGAG
481ACAGCCACCT TACACTCCTA AACCAGCTGC TGCAGGGTTT GCGGCAGGAC CTCAGGCAAA
541GCCAGGAAGC AAGCAACCAC AGCTCCCACA CAGCTCAAAC ACCACGGATT TACCCAACTT
601TCTTGTACAA AGTtggcatt ataagaaagc attgcttatc aatttgttgc aacgaac

Download FASTA (ORF) (Full)