Transcript: Mouse NM_025301.2

Mus musculus mitochondrial ribosomal protein L17 (Mrpl17), mRNA.

Source:
NCBI, updated 2019-01-20
Taxon:
Mus musculus (mouse)
Gene:
Mrpl17 (27397)
Length:
6712
CDS:
107..637

Additional Resources:

NCBI RefSeq record:
NM_025301.2
NBCI Gene record:
Mrpl17 (27397)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_025301.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000246948 ATGGCGGTGATAGAATATAAA pLKO_005 467 CDS 100% 15.000 21.000 N Mrpl17 n/a
2 TRCN0000192965 GATGGCGGTGATAGAATATAA pLKO.1 466 CDS 100% 15.000 12.000 N Mrpl17 n/a
3 TRCN0000246949 TGAGTAGACATTGCCTAAATA pLKO_005 2889 3UTR 100% 15.000 10.500 N Mrpl17 n/a
4 TRCN0000246945 TTGATCCCAAAGCTGTTTAAA pLKO_005 359 CDS 100% 15.000 10.500 N Mrpl17 n/a
5 TRCN0000217035 CAAGTGAAGCTCAGCGTTATT pLKO.1 4999 3UTR 100% 13.200 9.240 N Mrpl17 n/a
6 TRCN0000246947 GCGGAGAAGCTCATCGATTAT pLKO_005 272 CDS 100% 13.200 9.240 N Mrpl17 n/a
7 TRCN0000216422 GTTCAAACACCAAAGACTTAA pLKO.1 617 CDS 100% 13.200 9.240 N Mrpl17 n/a
8 TRCN0000246946 GTTCAAACACCAAAGACTTAA pLKO_005 617 CDS 100% 13.200 9.240 N Mrpl17 n/a
9 TRCN0000201603 CAGCAACCTTACTCTCCTAAA pLKO.1 526 CDS 100% 10.800 7.560 N Mrpl17 n/a
10 TRCN0000192755 CCAAAGCTGTTTAAAGTGCTA pLKO.1 365 CDS 100% 2.640 1.848 N Mrpl17 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_025301.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_08808 pDONR223 100% 84.6% 84% None (many diffs) n/a
2 ccsbBroad304_08808 pLX_304 0% 84.6% 84% V5 (many diffs) n/a
3 TRCN0000468987 TGCGTAGTGGTGGACTTACTCTGC pLX_317 85.6% 84.6% 84% V5 (many diffs) n/a
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