Construct: ORF ccsbBroadEn_08855
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017943.1_s300c1, BRDN0000396279
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- INF2 (64423)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 64423 | INF2 | inverted formin, FH2 and WH... | NM_032714.2 | 99.8% | 100% | 105C>T |
| 2 | human | 64423 | INF2 | inverted formin, FH2 and WH... | NM_001031714.4 | 18.8% | 18.8% | 105C>T;702_3720delinsG |
| 3 | human | 64423 | INF2 | inverted formin, FH2 and WH... | NM_022489.4 | 18.6% | 18.7% | 105C>T;702_3747delinsG |
| 4 | mouse | 70435 | Inf2 | inverted formin, FH2 and WH... | XM_006516224.3 | 16.2% | 17.2% | (many diffs) |
| 5 | mouse | 70435 | Inf2 | inverted formin, FH2 and WH... | NM_198411.2 | 16.1% | 17.1% | (many diffs) |
| 6 | mouse | 70435 | Inf2 | inverted formin, FH2 and WH... | XM_017315182.1 | 15.8% | 16.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 768
- ORF length:
- 702
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG 61 TTGGCATGTC GGTGAAGGAG GGCGCACAGC GCAAGTGGGC AGCGCTGAAG GAGAAGCTGG 121 GGCCACAGGA TTCGGACCCC ACGGAGGCCA ACCTGGAGAG CGCGGACCCT GAGCTGTGCA 181 TCCGGCTGCT CCAGATGCCC TCTGTGGTCA ACTACTCCGG CCTGCGCAAG CGCCTGGAGG 241 GCAGCGACGG CGGCTGGATG GTGCAGTTCC TGGAGCAGAG CGGCCTGGAC CTGCTGCTGG 301 AGGCGCTGGC GCGGCTGTCG GGCCGCGGCG TTGCACGTAT CTCCGACGCC CTGCTGCAGC 361 TCACCTGCGT CAGCTGCGTG CGCGCCGTCA TGAACTCGCG GCAGGGCATC GAGTACATCC 421 TCAGCAACCA GGGCTACGTG CGCCAGCTCT CCCAGGCCCT GGACACATCC AACGTGATGG 481 TGAAGAAGCA GGTGTTTGAG CTACTGGCTG CCCTGTGCAT CTACTCTCCC GAGGGCCACG 541 TGCTGACCCT GGACGCCCTG GACCACTACA AGACGGTGTG CAGCCAGCAG TACCGCTTCA 601 GCATTGTCAT GAACGAGCTC TCCGGCAGCG ACAACGTGCC CTACGTGGTC ACCCTGCTTA 661 GCGTGATCAA CGCCGTCATC TTGGGCCCCG AGGACCTGCG CGCGCGCACC CAGCTGCGGA 721 ACGAGTTTAT CGGGCTGCAG CTGCTGGACG TCCTGGCTCG CCTGCGGTGC CCAACTTTCT 781 TGTACAAAGT tggcattata agaaagcatt gcttatcaat ttgttgcaac gaac