Construct: ORF ccsbBroadEn_08916
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000417.1_s300c1, BRDN0000387150
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- METTL16 (79066)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79066 | METTL16 | methyltransferase like 16 | NM_024086.4 | 99.9% | 99.8% | 1436G>A |
| 2 | human | 79066 | METTL16 | methyltransferase like 16 | XM_005256801.3 | 61.1% | 61% | 0_1ins654;782G>A |
| 3 | human | 79066 | METTL16 | methyltransferase like 16 | XM_017025046.1 | 61.1% | 61% | 0_1ins654;782G>A |
| 4 | human | 79066 | METTL16 | methyltransferase like 16 | XM_024450928.1 | 61.1% | 61% | 0_1ins654;782G>A |
| 5 | mouse | 67493 | Mettl16 | methyltransferase like 16 | NM_026197.3 | 85.3% | 87.7% | (many diffs) |
| 6 | mouse | 67493 | Mettl16 | methyltransferase like 16 | XM_006533998.3 | 85.3% | 87.7% | (many diffs) |
| 7 | mouse | 67493 | Mettl16 | methyltransferase like 16 | XM_006533999.2 | 85.3% | 87.7% | (many diffs) |
| 8 | mouse | 67493 | Mettl16 | methyltransferase like 16 | XM_006534000.3 | 77.7% | 79.3% | (many diffs) |
| 9 | mouse | 67493 | Mettl16 | methyltransferase like 16 | XM_017314719.1 | 77.7% | 79.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1752
- ORF length:
- 1686
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG 61 TTGGCATGGC TCTGAGTAAA TCAATGCATG CAAGAAATAG ATACAAGGAC AAACCTCCTG 121 ACTTTGCATA TCTGGCATCC AAATATCCAG ATTTTAAGCA GCATGTTCAG ATAAATCTGA 181 ATGGAAGAGT GAGCCTTAAT TTTAAAGACC CCGAAGCAGT CAGAGCTCTG ACGTGTACTC 241 TCCTAAGGGA AGATTTTGGA CTTTCTATTG ATATTCCATT GGAGAGACTA ATTCCCACAG 301 TTCCCTTGAG ACTCAACTAT ATTCACTGGG TAGAAGATCT GATCGGTCAC CAGGATTCTG 361 ACAAAAGTAC TCTCCGAAGA GGAATTGACA TAGGCACGGG GGCATCTTGC ATCTACCCCT 421 TACTTGGAGC AACCTTGAAT GGCTGGTATT TCCTCGCAAC AGAAGTGGAT GATATGTGTT 481 TCAACTATGC AAAGAAAAAT GTGGAACAGA ATAACTTATC TGATCTCATA AAAGTGGTGA 541 AAGTGCCACA GAAGACACTC CTGATGGATG CTCTTAAAGA AGAATCTGAG ATAATCTATG 601 ACTTTTGCAT GTGCAACCCT CCCTTTTTTG CCAATCAATT GGAAGCCAAG GGAGTAAACT 661 CACGAAATCC TCGAAGACCT CCGCCTAGTT CTGTTAATAC AGGAGGCATC ACAGAGATCA 721 TGGCAGAAGG AGGTGAATTA GAGTTTGTTA AAAGGATCAT CCATGACAGT CTACAACTTA 781 AAAAAAGATT AAGATGGTAT AGCTGCATGC TGGGAAAGAA ATGCAGCCTG GCGCCTCTGA 841 AGGAGGAGCT TCGCATACAA GGGGTTCCCA AAGTAACGTA CACTGAATTC TGTCAAGGTC 901 GGACAATGAG ATGGGCCTTA GCTTGGAGTT TTTATGATGA TGTCACAGTA CCATCACCAC 961 CAAGTAAGCG AAGAAAATTA GAGAAACCGA GAAAACCCAT AACATTCGTG GTGCTGGCGT 1021 CCGTGATGAA GGAATTATCC CTCAAAGCAT CACCTCTGCG CTCGGAGACG GCGGAAGGCA 1081 TAGTCGTTGT CACGACATGG ATTGAAAAAA TTCTCACTGA TTTGAAGGTC CAGCATAAAC 1141 GAGTTCCCTG TGGAAAAGAG GAAGTCAGCC TTTTCCTAAC GGCCATAGAA AACTCCTGGA 1201 TTCATTTAAG GAGAAAGAAA AGAGAGCGTG TGAGACAGCT GAGAGAAGTT CCCCGAGCTC 1261 CTGAGGACGT CATTCAGGCC TTGGAAGAGA AAAAGCCCAC CCCCAAAGAG TCTGGCAATA 1321 GCCAAGAACT GGCCAGGGGC CCCCAGGAGA GGACCCCCTG TGGGCCTGCT CTGCGGGAAG 1381 GCGAGGCTGC CGCTGTGGAG GGCCCGTGCC CGAGCCAGGA GTCCCTGTCC CAGGAGGAAA 1441 ACCCGGAACC CACGGAGGAT GAAAGGAGTG AGGAAAAGGG AGGGGTGGAG GTTTTGGAAA 1501 ATTGTCAAGG CTCTAGCAAC GGAGCCCAGG ACCAAGAGGC TTCTGAGCAG TTCGGCAGCC 1561 CAGTGGCTGA AAGGGGGAAA CGTCTCCCAG GAGTGGCCGG ACAGTACCTG TTTAAGTGTT 1621 TGATAAACGT TAAGAAGGAG GTGGACGATG CCTTAGTGGA GATGCACTGG GTTGAGGGCC 1681 AGAACAGGGA TCTGATGAAC CAGCTTTGCA CCTACATACG TAACCAAATT TTCAGGCTTG 1741 TTGCAGTTAA CTACCCAACT TTCTTGTACA AAGTtggcat tataagaaag cattgcttat 1801 caatttgttg caacgaac