Construct: ORF ccsbBroadEn_09013

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF006543.1_s300c1, BRDN0000391128
DNA Barcode:
None
Epitope Tag:
None
Notes:
Has stop codon in insert

Originally Annotated References:

Gene:
OPA3 (80207)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 80207 OPA3 outer mitochondrial membran... NM_025136.4 99.8% 100% 231T>C
2 human 80207 OPA3 outer mitochondrial membran... NM_001017989.3 84.9% 77.3% (many diffs)
3 human 80207 OPA3 outer mitochondrial membran... XM_006723403.4 70.2% 70.3% 0_1ins159;72T>C
4 mouse 403187 Opa3 optic atrophy 3 NM_207525.3 82.4% 85.4% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
61
ORF end:
598
ORF length:
537
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaactttg tacaaaaAAG
61ATGGTGGTGG GCGCGTTCCC TATGGCGAAG CTGCTATACT TGGGCATCCG GCAGGTCAGC
121AAGCCGCTTG CCAACCGTAT TAAGGAGGCC GCCCGCCGAA GCGAGTTCTT CAAGACCTAT
181ATCTGCCTCC CGCCGGCTCA ACTGTATCAC TGGGTGGAGA TGCGGACCAA GATGCGCATC
241ATGGGCTTCC GGGGCACGGT CATCAAGCCG CTGAACGAGG AGGCGGCAGC CGAGCTGGGC
301GCAGAGCTGC TGGGCGAAGC CACCATCTTC ATCGTGGGCG GCGGCTGCCT AGTGCTGGAG
361TACTGGCGCC ACCAGGCGCA GCAGCGCCAC AAGGAGGAGG AGCAGCGTGC TGCCTGGAAC
421GCGCTGCGGG ACGAGGTGGG CCACCTGGCG CTGGCGCTGG AAGCGCTGCA GGCGCAGGTG
481CAGGCGGCGC CGCCACAGGG CGCCCTGGAG GAACTGCGCA CAGAGCTGCA AGAGGTGCGC
541GCCCAGCTCT GCAATCCCGG CCGGTCCGCT TCCCACGCAG TGCCTGCGTC CAAGAAATAG
601ccaactttct tgtacaaagt tggcattata agaaagcatt gcttatcaat ttgttgcaac
661gaac

Download FASTA (ORF) (Full)