Transcript: Human XM_006723403.4

PREDICTED: Homo sapiens outer mitochondrial membrane lipid metabolism regulator OPA3 (OPA3), transcript variant X1, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
OPA3 (80207)
Length:
5136
CDS:
222..602

Additional Resources:

NCBI RefSeq record:
XM_006723403.4
NBCI Gene record:
OPA3 (80207)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_006723403.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000083387 AGTGCCTGCGTCCAAGAAATA pLKO.1 581 CDS 100% 13.200 18.480 N OPA3 n/a
2 TRCN0000416790 AGGTGAAGCGTTGCAACATAG pLKO_005 776 3UTR 100% 10.800 15.120 N OPA3 n/a
3 TRCN0000083384 CGAGTTCTTCAAGACCTATAT pLKO.1 24 5UTR 100% 13.200 9.240 N OPA3 n/a
4 TRCN0000433523 CCTGATCAGTGTTAACCTTTA pLKO_005 955 3UTR 100% 10.800 7.560 N OPA3 n/a
5 TRCN0000083383 CCTGGTCTTGAGCTGAATCAA pLKO.1 904 3UTR 100% 5.625 3.938 N OPA3 n/a
6 TRCN0000007228 CACCTGTAATCCCAGCACTTT pLKO.1 2870 3UTR 100% 4.950 2.475 Y CFLAR n/a
7 TRCN0000166635 CACCTGTAATCCCAGCACTTT pLKO.1 2870 3UTR 100% 4.950 2.475 Y C19orf31 n/a
8 TRCN0000140719 GATCACTTGAGGTCAGGAGTT pLKO.1 1199 3UTR 100% 4.050 2.025 Y P3H4 n/a
9 TRCN0000165299 GATCACTTGAGGTCAGGAGTT pLKO.1 1199 3UTR 100% 4.050 2.025 Y ORAI2 n/a
10 TRCN0000352971 GATCACTTGAGGTCAGGAGTT pLKO_005 1199 3UTR 100% 4.050 2.025 Y P3H4 n/a
11 TRCN0000184236 GCGAGTTCTTCAAGACCTACA pLKO.1 23 5UTR 100% 4.050 2.835 N Opa3 n/a
12 TRCN0000256748 GGCAGGAGAATTGCTTGAATC pLKO_005 1326 3UTR 100% 10.800 5.400 Y SMIM11A n/a
13 TRCN0000155836 CCCAAAGTGCTGGGATTACAA pLKO.1 4198 3UTR 100% 5.625 2.813 Y KLHL30 n/a
14 TRCN0000021429 CACACCTGTAATCCCAGCATT pLKO.1 2868 3UTR 100% 4.950 2.475 Y ERN2 n/a
15 TRCN0000138998 CACACCTGTAATCCCAGCATT pLKO.1 2868 3UTR 100% 4.950 2.475 Y P3H4 n/a
16 TRCN0000344020 CACACCTGTAATCCCAGCATT pLKO_005 2868 3UTR 100% 4.950 2.475 Y P3H4 n/a
17 TRCN0000141025 CCCAAAGTGCTGGGATTACTT pLKO.1 4198 3UTR 100% 5.625 2.813 Y EID2B n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006723403.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_09013 pDONR223 98.3% 70.2% 70.3% None 0_1ins159;72T>C n/a
2 ccsbBroad304_09013 pLX_304 0% 70.2% 70.3% V5 (not translated due to prior stop codon) 0_1ins159;72T>C n/a
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