Construct: ORF ccsbBroadEn_09065
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001084.1_s300c1, BRDN0000455601
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MOB2 (81532)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 81532 | MOB2 | MOB kinase activator 2 | NM_053005.5 | 99.5% | 100% | 36T>C;225C>G;684G>A |
| 2 | human | 81532 | MOB2 | MOB kinase activator 2 | NM_001172223.3 | 87.2% | 86.1% | (many diffs) |
| 3 | mouse | 101513 | Mob2 | MOB kinase activator 2 | NM_028308.2 | 87.3% | 91.9% | (many diffs) |
| 4 | mouse | 101513 | Mob2 | MOB kinase activator 2 | XM_011241971.2 | 76.1% | 79.1% | (many diffs) |
| 5 | mouse | 101513 | Mob2 | MOB kinase activator 2 | NM_001347560.1 | 55.6% | 57.8% | (many diffs) |
| 6 | mouse | 101513 | Mob2 | MOB kinase activator 2 | XM_017321907.1 | 55.6% | 57.8% | (many diffs) |
| 7 | mouse | 101513 | Mob2 | MOB kinase activator 2 | XM_017321908.1 | 53.7% | 55% | (many diffs) |
| 8 | mouse | 101513 | Mob2 | MOB kinase activator 2 | XM_011241972.2 | 46.6% | 46.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 780
- ORF length:
- 711
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaacttTG TACAAAAAAG 61 TTGGCACCAT GGACTGGCTC ATGGGGAAGT CCAAAGCCAA GCCCAATGGC AAGAAGCCCG 121 CTGCGGAGGA GAGGAAGGCC TACCTGGAGC CTGAGCACAC CAAGGCCAGG ATCACCGACT 181 TCCAGTTCAA GGAGCTGGTG GTGCTGCCCC GCGAGATTGA CCTTAACGAG TGGCTGGCCA 241 GCAACACCAC GACGTTTTTC CACCACATCA ACCTGCAGTA TAGCACCATC TCGGAGTTCT 301 GCACAGGAGA GACGTGTCAG ACGATGGCCG TGTGCAACAC ACAGTACTAC TGGTATGACG 361 AGCGGGGGAA GAAGGTCAAG TGCACGGCCC CACAGTACGT TGACTTCGTC ATGAGCTCCG 421 TGCAGAAGCT GGTGACGGAT GAGGACGTGT TCCCCACAAA ATACGGCAGA GAATTCCCCA 481 GCTCCTTTGA GTCCCTGGTG AGGAAGATCT GCAGACACCT GTTCCACGTG CTGGCACACA 541 TCTACTGGGC CCACTTCAAG GAGACGCTGG CCCTGGAGCT GCACGGACAC TTGAACACGC 601 TCTACGTCCA CTTCATCCTC TTTGCTCGGG AGTTCAACCT GCTGGACCCC AAAGAGACCG 661 CCATCATGGA CGACCTCACC GAGGTGCTAT GCAGCGGGGC CGGCGGGGTC CACAGTGGGG 721 GCAGTGGGGA TGGGGCCGGC AGCGGGGGCC CAGGAGCACA GAACCACGTG AAGGAGAGAT 781 TGCCAACTTT CTTGTACAAA GTTGGcatta taagaaagca ttgcttatca atttgttgca 841 acgaac