Construct: ORF ccsbBroadEn_09184
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015508.1_s300c1, BRDN0000385501
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HPS3 (84343)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84343 | HPS3 | HPS3 biogenesis of lysosoma... | NM_032383.5 | 99.9% | 99.9% | 1494G>A;3002A>N |
| 2 | human | 84343 | HPS3 | HPS3 biogenesis of lysosoma... | XM_017007323.2 | 95.8% | 95.8% | 1494G>A;2888_2889delCTins125 |
| 3 | human | 84343 | HPS3 | HPS3 biogenesis of lysosoma... | XR_924201.3 | 89% | (many diffs) | |
| 4 | human | 84343 | HPS3 | HPS3 biogenesis of lysosoma... | XR_001740327.2 | 83.5% | (many diffs) | |
| 5 | human | 84343 | HPS3 | HPS3 biogenesis of lysosoma... | NM_001308258.2 | 83.4% | 83.4% | 216_217ins495;999G>A;2507A>N |
| 6 | human | 84343 | HPS3 | HPS3 biogenesis of lysosoma... | XR_001740326.2 | 78.3% | (many diffs) | |
| 7 | human | 84343 | HPS3 | HPS3 biogenesis of lysosoma... | XR_001740328.2 | 77.2% | (many diffs) | |
| 8 | human | 84343 | HPS3 | HPS3 biogenesis of lysosoma... | XM_005247834.4 | 75.9% | 75.9% | 1494G>A;2289_2290ins723 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 3078
- ORF length:
- 3012
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG 61 TTGGCATGGT GCAGCTGTAC AACCTGCACC CGTTCGGGTC GCAGCAGGTG GTGCCCTGCA 121 AGCTGGAGCC GGACCGGTTC TGTGGCGGGG GGCGTGACGC GCTTTTCGTG GCGGCGGGCT 181 GCAAGGTGGA GGCGTTCGCG GTGGCCGGCC AGGAGCTGTG CCAGCCGCGG TGCGCCTTCT 241 CCACGCTGGG CCGGGTGTTG CGCCTGGCCT ACAGCGAGGC TGGAGATTAT TTGGTAGCAA 301 TTGAAGAGAA AAACAAAGCT ACATTTCTAC GTGCTTATGT GAACTGGAGA AATAAAAGGA 361 CTGAAAACTC TCGTGTGTGT ATCCGAATGA TTGGGCATAA TGTGGAGGGA CCATTCAGCA 421 AAGCCTTCAG AGACCAGATG TACATTATTG AAATGCCGCT TTCGGAGGCC CCCTTGTGCA 481 TTTCCTGTTG CCCTGTGAAA GGAGACCTTC TCGTTGGCTG CACAAATAAA TTAGTCTTAT 541 TTAGTTTGAA GTACCAGATC ATTAATGAGG AATTCTCACT ATTGGACTTT GAACGTTCTT 601 TAATTATACA CATAGATAAT ATCACTCCTG TTGAGGTTTC TTTTTGTGTT GGATATGTTG 661 CTGTCATGTC AGACTTAGAA GTCTTAATCG TAAAACTGGA GTCAGGCCCT AAAAATGGAG 721 AGAGAGTTCA CCACCATCCA CATAAGACCA ACAATCGAAT AAGACGGACA GAAGAAGGCA 781 TCAGTAATGA AATTTCACAG CTTGAGTCAG ATGATTTTGT CATCTGCCAG AAGCCCCTGG 841 AACTTCTTGG TGAAAAAAGT GAACAGTCTG GATTATCTGT TACACTGGAG TCTACGGGAT 901 TAGCTGATGA AAAAAGAAAA TATTCCCACT TTCAGCACCT GCTCTATAGA CGTTTTGCTC 961 CTGATATTTC GTCCTATGTC TTGTCTGATG ACATCAAGCT ACATTCCCTC CAGCTGCTAC 1021 CCATTTACCA GACCGGTTCT CTTACATCTG ATGGAAAAAA TTTGTCTCAG GAAAAAGAAT 1081 TGCTGAGTCT CTTTTGCTTT TTCTCCTTAC CTCATGTGGG CTATCTCTAC ATGGTTGTCA 1141 AATCTGTTGA ATTGATGTCA GTCTACCAGT ATCCTGAAAA GTCTCAGCAG GCAGTACTCA 1201 CGCCACAATT TTTGCACGTC ATTACAAGTA ACAACCTGCA GTGTTTCACT GTGCGGTGCA 1261 GTGCGGCGGC AGCTCGTGAG GAGGACCCGT ACATGGACAC CACCCTGAAG GCTTGCCCAC 1321 CTGTCAGTAT GGATGTCTGT GCTTTAAGAA TACAGCTTTT CATAGGCTTG AAAGCCATCT 1381 GTCACTTTAA AAACCACATC ATACTTTTGA CTAAAGCAGA ACCTGAAGCC ATTCCAGAGA 1441 GAAGACAGTC ACCCAAGAGG CTTCTTTCGA GAAAAGATAC CAGTGTTAAA ATCAAAATAC 1501 CTCCTGTAGC TGAGGCTGGG TGGAATTTGT ATATTGTGAA TACGATCTCA CCAGTGCAAC 1561 TGTACAAAGA GATGGTAGAC TATAGCAATA CCTATAAGAC TGTCAAAACC CAGAGCTGCA 1621 TTCACCTTCT CAGTGAGGCT CATCTGTTAG TGCGAGCTGC CCTGATGGAT GCCAGTCAGC 1681 TGGAACCTGG AGAGAAGGCA GAGCTTTTGG AAGCATTTAA GGAAAGCTGT GGGCACCTTG 1741 GGGACTGTTA CAGCAGGCTT GACTCCCAGC ATTCTCATCT CACCTTGCCA TACTATAAGA 1801 TGTCTGGTTT GTCTATGGCT GAAGTTCTGG CCCGCACGGA CTGGACAGTA GAGGATGGAT 1861 TACAGAAATA CGAGAGAGGA TTAATCTTTT ACATTAATCA TTCACTTTAT GAAAACCTGG 1921 ATGAAGAATT AAATGAAGAA TTAGCAGCAA AAGTGGTTCA GATGTTTTAT GTGGCTGAGC 1981 CAAAGCAAGT GCCCCATATT CTCTGTAGTC CTTCTATGAA GAATATTAAT CCTTTAACTG 2041 CCATGAGCTA TCTAAGGAAG CTGGATACTT CTGGGTTTTC ATCGATCTTA GTGACATTGA 2101 CCAAGGCAGC AGTGGCTCTG AAAATGGGAG ATCTTGACAT GCACAGAAAT GAAATGAAAA 2161 GCCATTCAGA GATGAAGTTG GTATGTGGCT TCATTCTGGA ACCTCGGCTG TTGATTCAAC 2221 AGAGAAAGGG ACAGATTGTT CCAACCGAGC TTGCACTTCA CTTGAAGGAA ACTCAGCCTG 2281 GATTGCTTGT GGCTTCAGTT CTGGGCTTGC AGAAGAACAA CAAAATTGGA ATTGAAGAAG 2341 CAGATTCCTT TTTTAAGGTG CTTTGTGCTA AGGATGAAGA TACAATTCCT CAGCTCTTGG 2401 TAGACTTTTG GGAAGCTCAG CTAGTGGCAT GTCTCCCAGA TGTGGTACTT CAGGAACTCT 2461 TTTTCAAACT CACATCACAG TACATCTGGA GATTGTCTAA GAGGCAGCCT CCTGACACCA 2521 CACCATTGCG AACATCGGAG GATCTGATAA ATGCCTGTAG TCATTATGGC TTAATTTATC 2581 CATGGGTTCA CGTCGTAATA TCATCTGATT CTTTAGCTGA TAAAAATTAT ACAGAAGATC 2641 TTTCAAAATT ACAGTCTCTT ATATGTGGTC CTTCATTTGA CATAGCTTCC ATTATTCCGT 2701 TCTTGGAGCC ACTTTCAGAA GACACTATTG CCGGCCTCAG TGTCCATGTT CTGTGTCGTA 2761 CACGCTTGAA AGAGTATGAA CAGTGCATAG ACATACTGTT AGAGAGATGC CCGGAGGCAG 2821 TCATTCCATA TGCTAATCAT GAACTGAAAG AAGAGAACCG GACTCTGTGG TGGAAAAAAC 2881 TGTTGCCTGA ACTTTGTCAG AGAATAAAAT GTGGTGGAGA GAAGTATCAA CTCTACCTGT 2941 CATCATTAAA AGAAACATTG TCAATTGTTG CTGTGGAACT AGAACTGAAG GATTTCATGA 3001 ATGTTCTCCC AGAAGATGGT ACTGCAACAT TTTTCTTGCC ATATCTTCTC TATTGCAGTC 3061 GAAAGANACC ATTGACTTAC CCAACTTTCT TGTACAAAGT tggcattata agaaagcatt 3121 gcttatcaat ttgttgcaac gaac