Construct: ORF ccsbBroadEn_09193
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006545.1_s300c1, BRDN0000457359
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MEGF10 (84466)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84466 | MEGF10 | multiple EGF like domains 10 | NM_001256545.2 | 99.9% | 99.9% | 25C>G;1114C>T;3387T>A |
| 2 | human | 84466 | MEGF10 | multiple EGF like domains 10 | NM_032446.3 | 99.9% | 99.9% | 25C>G;1114C>T;3387T>A |
| 3 | human | 84466 | MEGF10 | multiple EGF like domains 10 | XM_011543694.1 | 99.9% | 99.9% | 25C>G;1114C>T;3387T>A |
| 4 | human | 84466 | MEGF10 | multiple EGF like domains 10 | XM_017009987.1 | 95.3% | 95.3% | (many diffs) |
| 5 | human | 84466 | MEGF10 | multiple EGF like domains 10 | XM_017009988.1 | 66.6% | 66.6% | 0_1ins1140;2247T>A |
| 6 | human | 84466 | MEGF10 | multiple EGF like domains 10 | NM_001308119.1 | 49.5% | 49.4% | (many diffs) |
| 7 | human | 84466 | MEGF10 | multiple EGF like domains 10 | NM_001308121.1 | 49.5% | 49.4% | (many diffs) |
| 8 | mouse | 70417 | Megf10 | multiple EGF-like-domains 10 | NM_001001979.2 | 87.1% | 94% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 3489
- ORF length:
- 3420
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaacttTG TACAAAAAAG 61 TTGGCACCAT GGTTATTTCT TTGAACTCAT GCGTGAGCTT TATTTGTTTA TTGTTATGCC 121 ACTGGATTGG GACAGCATCA CCTCTGAATC TTGAAGACCC TAATGTGTGT AGCCACTGGG 181 AAAGCTACTC AGTGACTGTG CAAGAGTCAT ACCCACATCC CTTTGATCAA ATTTACTACA 241 CGAGCTGCAC TGACATTCTA AACTGGTTTA AATGCACGCG GCACAGAGTC AGCTATCGGA 301 CAGCCTATCG ACATGGGGAG AAGACTATGT ATAGGCGCAA GTCTCAGTGT TGTCCTGGAT 361 TTTATGAAAG CGGGGAAATG TGTGTCCCCC ACTGTGCTGA TAAATGTGTC CATGGTCGCT 421 GTATTGCTCC AAACACCTGT CAGTGTGAGC CTGGCTGGGG AGGGACCAAC TGCTCCAGTG 481 CCTGCGATGG TGATCACTGG GGTCCCCACT GCACCAGCCG GTGCCAGTGC AAAAATGGGG 541 CTCTGTGCAA CCCCATCACC GGGGCTTGCC ACTGTGCTGC GGGCTTCCGG GGCTGGCGCT 601 GCGAGGACCG CTGTGAGCAG GGCACCTATG GTAACGACTG TCATCAGAGA TGCCAGTGCC 661 AGAATGGAGC CACCTGCGAC CACGTCACGG GGGAATGCCG CTGCCCACCA GGATACACCG 721 GAGCCTTCTG TGAGGATCTT TGTCCTCCTG GTAAACATGG TCCACAGTGT GAGCAGAGAT 781 GCCCTTGTCA AAATGGAGGA GTGTGTCATC ACGTCACTGG AGAATGCTCT TGCCCTTCTG 841 GCTGGATGGG CACAGTGTGT GGTCAGCCTT GCCCCGAGGG TCGCTTTGGA AAGAACTGTT 901 CCCAAGAATG CCAGTGCCAT AATGGAGGGA CGTGTGATGC TGCCACAGGC CAATGTCATT 961 GCAGTCCAGG ATACACAGGG GAACGGTGCC AGGATGAGTG TCCTGTTGGG ACCTATGGCG 1021 TTCTCTGTGC TGAGACCTGC CAGTGTGTCA ACGGAGGGAA GTGTTACCAC GTGAGCGGCG 1081 CATGCCTCTG TGAAGCAGGC TTTGCTGGCG AGCGCTGCGA AGCACGCCTG TGTCCTGAGG 1141 GGCTCTACGG CATCAAATGT GACAAACGGT GTCCCTGCCA CTTGGAAAAC ACTCATAGCT 1201 GTCACCCCAT GTCTGGAGAG TGTGCCTGCA AGCCGGGCTG GTCAGGACTC TACTGTAATG 1261 AGACATGTTC TCCTGGATTC TACGGGGAAG CTTGCCAGCA GATCTGCAGC TGCCAAAATG 1321 GGGCAGACTG TGACAGTGTG ACTGGAAAGT GCACCTGTGC CCCAGGATTC AAAGGAATTG 1381 ACTGCTCTAC CCCATGCCCT CTGGGAACCT ATGGGATAAA CTGTTCCTCT CGCTGTGGCT 1441 GTAAAAATGA TGCAGTCTGC TCTCCTGTGG ACGGGTCTTG TACTTGCAAG GCAGGCTGGC 1501 ACGGGGTGGA CTGCTCCATC AGATGTCCCA GTGGCACATG GGGCTTTGGC TGTAACTTAA 1561 CATGCCAGTG CCTCAACGGG GGAGCCTGCA ACACCCTGGA CGGGACCTGC ACGTGTGCAC 1621 CTGGATGGCG CGGGGAGAAA TGCGAACTTC CCTGCCAGGA TGGCACGTAC GGGCTGAACT 1681 GTGCTGAGCG CTGCGACTGC AGCCACGCAG ATGGCTGCCA CCCTACCACG GGCCATTGCC 1741 GCTGCCTCCC CGGATGGTCA GGTGTCCACT GTGACAGCGT GTGTGCTGAG GGACGCTGGG 1801 GCCCCAACTG CTCCCTGCCC TGCTACTGTA AAAATGGGGC TTCATGCTCC CCTGATGATG 1861 GCATCTGCGA GTGTGCACCA GGCTTCCGAG GCACCACTTG TCAGAGGATC TGCTCCCCTG 1921 GTTTTTATGG GCATCGCTGC AGCCAGACAT GCCCACAGTG CGTTCACAGC AGCGGGCCCT 1981 GCCACCACAT CACCGGCCTG TGTGACTGCT TGCCTGGCTT CACAGGCGCC CTCTGCAATG 2041 AAGTGTGTCC CAGTGGCAGA TTTGGGAAAA ACTGTGCAGG AATTTGTACC TGCACCAACA 2101 ACGGAACCTG TAACCCCATT GACAGATCTT GTCAGTGTTA CCCCGGTTGG ATTGGCAGTG 2161 ACTGCTCTCA ACCATGTCCA CCTGCCCACT GGGGCCCAAA CTGCATCCAC ACGTGCAACT 2221 GCCATAATGG AGCTTTCTGC AGCGCCTACG ATGGGGAATG TAAATGCACT CCTGGCTGGA 2281 CAGGGCTCTA CTGCACTCAG AGATGTCCTC TAGGGTTTTA TGGAAAAGAT TGTGCACTGA 2341 TATGCCAATG TCAAAACGGA GCTGACTGCG ACCACATTTC TGGGCAGTGT ACTTGCCGCA 2401 CTGGATTCAT GGGACGGCAC TGTGAGCAGA AGTGCCCTTC AGGAACATAT GGCTATGGCT 2461 GTCGCCAGAT ATGTGATTGT CTGAACAACT CCACCTGCGA CCACATCACT GGGACCTGTT 2521 ACTGCAGCCC CGGATGGAAG GGAGCGAGAT GTGATCAAGC TGGTGTTATC ATAGTTGGAA 2581 ATCTGAACAG CTTAAGCCGA ACCAGTACTG CTCTCCCTGC TGATTCCTAC CAGATCGGGG 2641 CCATTGCAGG CATCATCATT CTTGTCCTAG TTGTTCTCTT CCTACTGGCA TTGTTCATTA 2701 TTTATAGACA CAAGCAGAAG GGAAAGGAAT CAAGCATGCC AGCAGTTACC TACACCCCTG 2761 CTATGAGGGT CGTCAATGCA GATTATACCA TTTCAGGAAC CCTTCCTCAC AGCAATGGTG 2821 GAAACGCTAA TAGCCACTAC TTCACCAATC CCAGTTACCA CACGCTCACC CAGTGTGCCA 2881 CATCCCCTCA CGTCAACAAC AGGGACAGGA TGACTGTCAC GAAGTCAAAA AACAATCAAC 2941 TGTTTGTGAA TCTTAAAAAT GTGAACCCTG GGAAGAGAGG CCCTGTGGGG GACTGCACTG 3001 GGACATTGCC GGCTGACTGG AAACATGGCG GCTACCTCAA CGAGCTCGGT GCTTTTGGAC 3061 TTGACAGAAG CTATATGGGA AAATCCTTAA AAGACCTGGG AAAGAATTCT GAATATAATT 3121 CAAGTAACTG CTCCCTAAGC AGTTCTGAGA ACCCATATGC CACTATTAAA GACCCACCTG 3181 TACTTATCCC GAAAAGCTCA GAGTGTGGTT ATGTGGAGAT GAAATCGCCG GCACGAAGAG 3241 ATTCCCCATA TGCAGAGATC AATAACTCAA CTTCAGCCAA CAGGAATGTC TATGAAGTTG 3301 AACCTACAGT GAGTGTTGTC CAAGGAGTAT TCAGCAATAA TGGGCGTCTC TCCCAGGATC 3361 CATATGACCT CCCAAAGAAC AGTCACATCC CTTGTCATTA TGACCTGCTG CCAGTCCGAG 3421 ACAGTTCATC CTCCCCTAAG CAAGAGGACA GTGGAGGTAG CAGCAGCAAC AGCAGCAGCA 3481 GCAGTGAATT GCCAACTTTC TTGTACAAAG TTGGcattat aagaaagcat tgcttatcaa 3541 tttgttgcaa cgaac