Construct: ORF ccsbBroadEn_09476

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF004216.1_s300c1, BRDN0000455936
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
DEGS2 (123099)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 123099 DEGS2 delta 4-desaturase, sphingo... NM_206918.3 99.7% 99.6% 169G>A;465A>G
2 human 123099 DEGS2 delta 4-desaturase, sphingo... XM_006720043.3 88.6% 88.5% 0_1ins108;61G>A;357A>G
3 mouse 70059 Degs2 delta(4)-desaturase, sphing... NM_027299.5 82.5% 86% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1038
ORF length:
969
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaacttTG TACAAAAAAG
61TTGGCACCAT GGGCAACAGC GCGAGCCGCA GCGACTTCGA GTGGGTCTAC ACCGACCAGC
121CGCACACGCA GCGGCGCAAG GAGATACTGG CCAAGTACCC GGCCATCAAG GCCCTGATGC
181GGCCAGACCC GCGCCTCAAG TGGGCGGTGC TGGTGCTGGT GCTGGTGCAG ATGCTGACCT
241GCTGGCTGGT GCGCGGGCTG GCCTGGCGCT GGCTGCTGTT CTGGGCCTAC GCCTTTGGTG
301GCTGCGTGAA CCACTCGCTG ACGCTGGCCA TCCACGACAT CTCGCACAAC GCGGCCTTCG
361GCACGGGCCG TGCGGCACGC AACCGCTGGC TGGCCGTGTT CGCCAACCTG CCCGTGGGTG
421TGCCCTACGC CGCCTCCTTC AAGAAGTACC ACGTGGACCA CCACCGCTAC CTGGGCGGCG
481ACGGGCTGGA CGTGGACGTG CCCACGCGTC TGGAGGGCTG GTTCTTCTGC ACGCCCGCCC
541GCAAGCTGCT CTGGCTGGTG CTGCAGCCCT TCTTCTACTC ACTACGGCCG CTCTGCGTCC
601ACCCCAAGGC CGTGACCCGC ATGGAGGTGC TCAACACGCT GGTGCAGCTG GCGGCCGACC
661TGGCCATCTT TGCCCTTTGG GGGCTCAAGC CCGTGGTCTA CCTGCTGGCC AGCTCCTTCC
721TGGGCCTGGG CCTGCACCCC ATCTCGGGCC ACTTCGTGGC CGAGCACTAC ATGTTCCTCA
781AGGGCCACGA GACCTACTCC TACTATGGGC CTCTCAACTG GATCACCTTC AATGTGGGCT
841ACCACGTGGA GCACCACGAC TTCCCCAGCA TCCCGGGCTA CAACCTGCCG CTGGTGCGGA
901AGATCGCGCC CGAGTACTAC GACCACCTGC CGCAGCACCA CTCCTGGGTG AAGGTGCTCT
961GGGATTTTGT GTTTGAGGAC TCCCTGGGGC CCTATGCCAG GGTGAAGCGG GTGTACAGGC
1021TGGCAAAAGA TGGTCTGTTG CCAACTTTCT TGTACAAAGT TGGcattata agaaagcatt
1081gcttatcaat ttgttgcaac gaac

Download FASTA (ORF) (Full)