Construct: ORF ccsbBroadEn_09862

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF012601.1_s300c1, BRDN0000392276
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
MARCHF8 (220972)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 220972 MARCHF8 membrane associated ring-CH... NM_001002266.3 99.8% 99.6% 796T>C
2 human 220972 MARCHF8 membrane associated ring-CH... NM_145021.5 99.8% 99.6% 796T>C
3 human 220972 MARCHF8 membrane associated ring-CH... XM_011539495.1 99.8% 99.6% 796T>C
4 human 220972 MARCHF8 membrane associated ring-CH... NM_001282866.2 50.7% 50.6% 236_1081del;1642T>C
5 human 220972 MARCHF8 membrane associated ring-CH... XM_005271804.3 50.7% 50.6% 236_1081del;1642T>C
6 human 220972 MARCHF8 membrane associated ring-CH... XM_006717704.2 50.7% 50.6% 236_1081del;1642T>C
7 human 220972 MARCHF8 membrane associated ring-CH... XM_011539492.3 50.7% 50.6% 236_1081del;1642T>C
8 human 220972 MARCHF8 membrane associated ring-CH... XR_246519.3 13.9% (many diffs)
9 mouse 71779 March8 membrane-associated ring fi... NM_001302383.1 87.8% 91.4% (many diffs)
10 mouse 71779 March8 membrane-associated ring fi... NM_001302384.1 87.8% 91.4% (many diffs)
11 mouse 71779 March8 membrane-associated ring fi... NM_027920.5 87.8% 91.4% (many diffs)
12 mouse 71779 March8 membrane-associated ring fi... XM_006506648.3 79.7% 82.5% (many diffs)
13 mouse 71779 March8 membrane-associated ring fi... NM_001302385.1 75.5% 78.7% (many diffs)
14 mouse 71779 March8 membrane-associated ring fi... XM_011241470.2 75.5% 78.7% (many diffs)
15 mouse 71779 March8 membrane-associated ring fi... XM_017321749.1 75.5% 78.7% (many diffs)
16 mouse 71779 March8 membrane-associated ring fi... XM_006506643.3 45% 41.9% (many diffs)
17 mouse 71779 March8 membrane-associated ring fi... XM_006506638.3 44.7% 46.5% (many diffs)
18 mouse 71779 March8 membrane-associated ring fi... XM_006506639.3 44.7% 46.5% (many diffs)
19 mouse 71779 March8 membrane-associated ring fi... XM_006506640.3 44.7% 46.5% (many diffs)
20 mouse 71779 March8 membrane-associated ring fi... XM_006506641.3 44.7% 46.5% (many diffs)
21 mouse 71779 March8 membrane-associated ring fi... XM_006506642.1 44.7% 46.5% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
939
ORF length:
873
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGAG CATGCCACTG CATCAGATCT CTGCCATTCC ATCCCAGGAT GCCATCTCTG
121CTAGAGTCTA CAGAAGTAAG ACCAAAGAAA AGGAGAGGGA AGAACAGAAT GAGAAGACTT
181TGGGACATTT CATGAGTCAT TCAAGCAACA TTTCTAAGGC TGGGAGTCCT CCGTCAGCAT
241CAGCTCCGGC TCCGGTGTCC TCCTTCTCTC GCACTTCTAT CACGCCATCC AGCCAGGACA
301TCTGCAGGAT CTGCCACTGT GAAGGAGATG ATGAGAGCCC CCTGATCACC CCCTGCCACT
361GCACAGGAAG CCTCCACTTC GTGCACCAGG CCTGCCTGCA GCAGTGGATC AAGAGCTCCG
421ACACGCGCTG CTGCGAGCTC TGCAAGTATG AGTTCATCAT GGAGACCAAG CTGAAGCCAC
481TGAGAAAATG GGAGAAGTTG CAGATGACGT CCAGCGAGCG CAGGAAGATC ATGTGCTCAG
541TGACATTCCA CGTCATTGCC ATCACATGTG TGGTCTGGTC CTTGTATGTG CTCATTGACC
601GTACTGCTGA GGAGATCAAG CAGGGGCAGG CAACAGGAAT CCTAGAATGG CCCTTTTGGA
661CTAAATTGGT GGTTGTGGCC ATCGGCTTCA CCGGAGGACT TCTTTTTATG TATGTTCAGT
721GTAAAGTGTA TGTGCAATTG TGGAAGAGAC TCAAGGCCTA TAATAGAGTG ATCTATGTTC
781AAAACTGTCC AGAAACAAGC AAAAAGAATA TTTTTGAAAA ATCTCCACTA ACAGAGCCCA
841ACTTTGAAAA TAAACATGGA CATGGAATCT GTCATTCCGA CACAAACTCT TCTTGTTGCA
901CAGAGCCTGA AGACACTGGA GCAGAAATCA TTCACGTCTG CCCAACTTTC TTGTACAAAG
961Ttggcattat aagaaagcat tgcttatcaa tttgttgcaa cgaac

Download FASTA (ORF) (Full)