Construct: ORF ccsbBroadEn_09862

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF012601.1_s300c1, BRDN0000392276
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
MARCHF8 (220972)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 220972 MARCHF8 membrane associated ring-CH... NM_001002266.3 99.8% 99.6% 796T>C
2 human 220972 MARCHF8 membrane associated ring-CH... NM_145021.5 99.8% 99.6% 796T>C
3 human 220972 MARCHF8 membrane associated ring-CH... XM_011539495.1 99.8% 99.6% 796T>C
4 human 220972 MARCHF8 membrane associated ring-CH... NM_001282866.2 50.7% 50.6% 236_1081del;1642T>C
5 human 220972 MARCHF8 membrane associated ring-CH... XM_005271804.3 50.7% 50.6% 236_1081del;1642T>C
6 human 220972 MARCHF8 membrane associated ring-CH... XM_006717704.2 50.7% 50.6% 236_1081del;1642T>C
7 human 220972 MARCHF8 membrane associated ring-CH... XM_011539492.3 50.7% 50.6% 236_1081del;1642T>C
8 human 220972 MARCHF8 membrane associated ring-CH... XR_246519.3 13.9% (many diffs)
9 mouse 71779 March8 membrane-associated ring fi... NM_001302383.1 87.8% 91.4% (many diffs)
10 mouse 71779 March8 membrane-associated ring fi... NM_001302384.1 87.8% 91.4% (many diffs)
11 mouse 71779 March8 membrane-associated ring fi... NM_027920.5 87.8% 91.4% (many diffs)
12 mouse 71779 March8 membrane-associated ring fi... XM_006506648.3 79.7% 82.5% (many diffs)
13 mouse 71779 March8 membrane-associated ring fi... NM_001302385.1 75.5% 78.7% (many diffs)
14 mouse 71779 March8 membrane-associated ring fi... XM_011241470.2 75.5% 78.7% (many diffs)
15 mouse 71779 March8 membrane-associated ring fi... XM_017321749.1 75.5% 78.7% (many diffs)
16 mouse 71779 March8 membrane-associated ring fi... XM_006506643.3 45% 41.9% (many diffs)
17 mouse 71779 March8 membrane-associated ring fi... XM_006506638.3 44.7% 46.5% (many diffs)
18 mouse 71779 March8 membrane-associated ring fi... XM_006506639.3 44.7% 46.5% (many diffs)
19 mouse 71779 March8 membrane-associated ring fi... XM_006506640.3 44.7% 46.5% (many diffs)
20 mouse 71779 March8 membrane-associated ring fi... XM_006506641.3 44.7% 46.5% (many diffs)
21 mouse 71779 March8 membrane-associated ring fi... XM_006506642.1 44.7% 46.5% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
939
ORF length:
873
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGAG CATGCCACTG CATCAGATCT CTGCCATTCC ATCCCAGGAT GCCATCTCTG
121CTAGAGTCTA CAGAAGTAAG ACCAAAGAAA AGGAGAGGGA AGAACAGAAT GAGAAGACTT
181TGGGACATTT CATGAGTCAT TCAAGCAACA TTTCTAAGGC TGGGAGTCCT CCGTCAGCAT
241CAGCTCCGGC TCCGGTGTCC TCCTTCTCTC GCACTTCTAT CACGCCATCC AGCCAGGACA
301TCTGCAGGAT CTGCCACTGT GAAGGAGATG ATGAGAGCCC CCTGATCACC CCCTGCCACT
361GCACAGGAAG CCTCCACTTC GTGCACCAGG CCTGCCTGCA GCAGTGGATC AAGAGCTCCG
421ACACGCGCTG CTGCGAGCTC TGCAAGTATG AGTTCATCAT GGAGACCAAG CTGAAGCCAC
481TGAGAAAATG GGAGAAGTTG CAGATGACGT CCAGCGAGCG CAGGAAGATC ATGTGCTCAG
541TGACATTCCA CGTCATTGCC ATCACATGTG TGGTCTGGTC CTTGTATGTG CTCATTGACC
601GTACTGCTGA GGAGATCAAG CAGGGGCAGG CAACAGGAAT CCTAGAATGG CCCTTTTGGA
661CTAAATTGGT GGTTGTGGCC ATCGGCTTCA CCGGAGGACT TCTTTTTATG TATGTTCAGT
721GTAAAGTGTA TGTGCAATTG TGGAAGAGAC TCAAGGCCTA TAATAGAGTG ATCTATGTTC
781AAAACTGTCC AGAAACAAGC AAAAAGAATA TTTTTGAAAA ATCTCCACTA ACAGAGCCCA
841ACTTTGAAAA TAAACATGGA CATGGAATCT GTCATTCCGA CACAAACTCT TCTTGTTGCA
901CAGAGCCTGA AGACACTGGA GCAGAAATCA TTCACGTCTG CCCAACTTTC TTGTACAAAG
961Ttggcattat aagaaagcat tgcttatcaa tttgttgcaa cgaac

Download FASTA (ORF) (Full)

GPP Web Portal Terms of Service

Effective Date: December 8, 2025
By using this site, you agree to our terms and conditions below.

Overview of Terms

The data made available on this website were generated for research purposes and are not intended for clinical or commercial uses. Commercial use (or other use for profit-making purposes) of the GPP Web Portal and its tools, is not permitted under these terms and may require a separate license agreement from Broad or its contributors. For more information, please contact partnering@broadinstitute.org.

The original data may be subject to rights claimed by third parties, including but not limited to, patent, copyright, other intellectual property rights, biodiversity-related access and benefit-sharing rights. It is the responsibility of users of Broad Institute services to ensure that their use of the data does not infringe any of the rights of such third parties.

Any questions or comments concerning these Terms of Use can be addressed to: legal@broadinstitute.org.

By accessing and viewing this GPP Web Portal, you agree to the following terms and conditions:

Attribution

You agree to acknowledge the Broad Institute (e.g., in publications, services or products) for any of your use of its online services, databases or software in accordance with good scientific practice. You agree to use the acknowledgment wording provided for the relevant tools as indicated on the FAQ for each tool.

Updating the Terms of Use

We reserve the right to update these Terms of Use at any time. When alterations are inevitable, we will attempt to give reasonable notice of any changes by placing a notice on our website, but you may wish to check each time you use the website. The date of the most recent revision will appear on this, the "GPP Web Portal Terms of Use" page. If you do not agree to these changes, please do not continue to use our online services. We will also make available an archived copy of the previous Terms of Use for comparison.

Indemnification and Disclaimer of Warranties

You are using this GPP Web Portal at your own risk, and you hereby agree to hold Broad and its contributors and their trustees, directors, officers, employees, and affiliated investigators harmless for any third party claims which may arise from your use of the GPP Web Portal, the tools available therein, or any portion thereof. Further, you agree to indemnify Broad, its contributors, and its and their trustees, directors, officers, employees, affiliated investigators, students, and affiliates for any loss, costs, claims, damages, or other liabilities arising from any unpermitted commercial or profit-making use you make of the GPP Web Portal. The GPP Web Portal is a research tool and is provided "as is". Broad does not represent that the GPP Web Portal is free of errors or bugs or suitable for any particular tasks.

ANY EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT LIMITED TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE, NONINFRINGEMENT, OR THE ABSENCE OF LATENT OR OTHER DEFECTS ARE DISCLAIMED. IN NO EVENT SHALL BROAD OR ITS CONTRIBUTORS BE LIABLE FOR ANY DIRECT, INDIRECT, INCIDENTAL, SPECIAL, EXEMPLARY, OR CONSEQUENTIAL DAMAGES HOWEVER CAUSED AND ON ANY THEORY OF LIABILITY, WHETHER IN CONTRACT, STRICT LIABILITY, OR TORT (INCLUDING NEGLIGENCE OR OTHERWISE) ARISING IN ANY WAY OUT OF THE USE OF THE GPP WEB PORTAL, EVEN IF ADVISED OF THE POSSIBILITY OF SUCH DAMAGE.

Governing Law

The terms and conditions herein shall be construed, governed, interpreted, and applied in accordance with the internal laws of the Commonwealth of Massachusetts, U.S.A. Furthermore, by accessing, downloading, or using the Database, You consent to the personal jurisdiction of, and venue in, the state and federal courts within Massachusetts with respect to Your download or use of the Database.