Construct: ORF ccsbBroadEn_09987

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF016363.1_s300c1, BRDN0000456974
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PNPLA1 (285848)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 285848 PNPLA1 patatin like phospholipase ... NM_001145716.2 99.7% 99.3% 1010C>A;1211C>T;1306T>C
2 human 285848 PNPLA1 patatin like phospholipase ... NM_173676.2 97.7% 97.3% (many diffs)
3 human 285848 PNPLA1 patatin like phospholipase ... XM_017010778.1 91.5% 91.1% (many diffs)
4 human 285848 PNPLA1 patatin like phospholipase ... XM_011514520.2 89.7% 89.3% (many diffs)
5 human 285848 PNPLA1 patatin like phospholipase ... XM_017010777.2 82.2% 81.8% (many diffs)
6 human 285848 PNPLA1 patatin like phospholipase ... NM_001145717.1 80.5% 80.2% (many diffs)
7 human 285848 PNPLA1 patatin like phospholipase ... XM_017010776.1 76.5% 76.2% (many diffs)
8 human 285848 PNPLA1 patatin like phospholipase ... XM_011514519.2 75% 74.6% (many diffs)
9 human 285848 PNPLA1 patatin like phospholipase ... XM_017010779.1 72.8% 72.4% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1407
ORF length:
1338
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaacttTG TACAAAAAAG
61TTGGCACCAT GGTGCAGATG ATGAGGCAGT TTCTGTACCG GGTCCTGCCC GAGGACTCCT
121ACAAGGTCAC CACGGGGAAG CTCCATGTGA GCCTCACCCG CTTAACGGAC GGGGAGAATG
181TGGTGGTTTC AGAGTTCACG TCCAAGGAGG AGCTCATTGA GGCCCTATAC TGCAGCTGCT
241TCGTCCCGGT GTACTGTGGC CTCATCCCCC CGACTTACCG CGGTGTGTGG GCATTTCTCA
301CTCTGCCCCC ACAGAGGTAC ATCGATGGGG GCTTCACGGG CATGCAGCCC TGTGCCTTCT
361GGACCGACGC CATCACCATC TCCACCTTCA GTGGGCAGCA GGACATCTGT CCCCGGGACT
421GCCCGGCCAT CTTCCACGAC TTCCGCATGT TCAACTGCTC CTTCCAGTTC TCCCTGGAGA
481ACATCGCCAG GATGACCCAC GCATTGTTCC CCCCGGACCT GGTGATCCTG CACGATTACT
541ACTACCGAGG GTACGAGGAT GCAGTTTTGT ACTTGAGGCG GCTGAATGCT GTTTATCTTA
601ATTCTTCCTC CAAGAGAGTG ATTTTCCCCC GGGTGGAAGT GTACTGCCAG ATAGAACTCG
661CCCTTGGCAA TGAGTGCCCT GAACGCAGTC AACCAAGCCT TCGAGCACGG CAGGCCAGTC
721TGGAAGGAGC CACACAACCT CACAAGGAGT GGGTTCCCAA AGGGGATGGA AGGGGCAGCC
781ATGGTCCGCC TGTGTCCCAA CCTGTGCAGA CACTTGAATT CACATGCGAG TCACCTGTTT
841CAGCACCAGT CTCTCCACTT GAGCAGCCAC CTGCACAGCC ACTGGCCTCT TCAACTCCAC
901TTTCTCTAAG TGGCATGCCA CCTGTATCAT TCCCAGCTGT GCACAAGCCA CCCAGCTCCA
961CACCTGGTTC ATCACTGCCC ACCCCACCAC CTGGACTGTC ACCTCTGTCA CCTCAGCAGC
1021AGGTACAACC GTCTGGATCA CCAGCCAGAT CCCTACACTC TCAGGCACCC ACTTCACACA
1081GGCCATCCCT GGGGCCTTCA ACTGTGGGGG CACCTCAAAC ACTGCCCCGA AGTTCTCTTT
1141CAGCCTTCCC TGCTCAGCCA CCTGTGGAGG AACTAGGCCA AGAACAGCCC CAAGCTGTAG
1201CTCTTCTTGT CTCTTCAAAA CCAAAAAGCG CCGTGCCTCT GGTTCATGTG AAGGAAACCG
1261TCAGCAAGCC TTATGTAATG GAGAGCCCTG CTGAAGACTC AAACTGGGTG AATAAGGTCT
1321TCAAGAAGAA CAAGCAAAAG ACAAGTGGCA CCAGAAAAGG CTTCCCAAGA CATCCGGGAT
1381CCAAAAAACC AAGCAGCAAA GTGCAGTTGC CAACTTTCTT GTACAAAGTT GGcattataa
1441gaaagcattg cttatcaatt tgttgcaacg aac

Download FASTA (ORF) (Full)