Construct: ORF ccsbBroadEn_10157

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF000797.1_s300c1, BRDN0000390658
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
LHX8 (431707)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 431707 LHX8 LIM homeobox 8 NM_001001933.1 99.9% 99.7% 890T>C
2 human 431707 LHX8 LIM homeobox 8 NM_001256114.2 97% 96.9% 0_1ins30;860T>C
3 human 431707 LHX8 LIM homeobox 8 XM_017001316.1 88.3% 86.4% (many diffs)
4 human 431707 LHX8 LIM homeobox 8 XM_017001317.1 88.3% 86.4% (many diffs)
5 mouse 16875 Lhx8 LIM homeobox protein 8 XM_006501072.3 83.4% 88.7% (many diffs)
6 mouse 16875 Lhx8 LIM homeobox protein 8 XM_017319470.1 83.4% 88.7% (many diffs)
7 mouse 16875 Lhx8 LIM homeobox protein 8 NM_010713.2 80.4% 84.6% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1134
ORF length:
1068
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGCA GATTCTGAGC AGGTGTCAGG GGCTCATGTC AGAGGAGTGC GGGCGGACTA
121CAGCCCTGGC GGCCGGGAGG ACTCGCAAAG GCGCCGGGGA AGAGGGACTG GTGAGCCCCG
181AGGGAGCGGG GGACGAGGAC TCGTGCTCCT CCTCGGCCCC GCTGTCCCCG TCGTCCTCGC
241CCCGGTCCAT GGCCTCGGGC TCCGGCTGCC CTCCTGGCAA GTGTGTGTGC AACAGTTGCG
301GCCTGGAGAT CGTGGACAAA TACCTTCTCA AGGTGAATGA CCTATGCTGG CATGTCCGGT
361GTCTCTCCTG CAGTGTTTGC AGAACCTCCC TAGGAAGGCA CACCAGCTGT TATATTAAAG
421ACAAAGACAT TTTCTGCAAA CTTGATTATT TCAGAAGGTA TGGAACTCGC TGCTCTCGAT
481GTGGGAGACA CATCCATTCT ACTGACTGGG TCCGGAGAGC CAAGGGGAAT GTCTATCACT
541TGGCATGCTT TGCCTGCTTT TCCTGCAAAA GGCAACTTTC CACAGGAGAG GAGTTTGCTT
601TGGTGGAAGA GAAAGTCCTC TGCAGAGTAC ATTATGACTG CATGCTGGAT AATTTAAAAA
661GAGAAGTAGA AAATGGGAAT GGGATTAGTG TGGAAGGTGC CCTCCTCACA GAGCAAGATG
721TTAACCATCC AAAACCAGCA AAAAGAGCTC GGACCAGCTT TACAGCAGAT CAGCTTCAGG
781TTATGCAAGC ACAATTTGCT CAGGACAACA ACCCAGATGC ACAGACACTC CAGAAATTGG
841CAGAAAGGAC AGGCTTGAGC AGACGTGTGA TACAGGTGTG GTTTCAGAAT TGTAGAGCAC
901GCCACAAGAA ACACGTCAGT CCTAATCACT CATCCTCCAC CCCAGTCACA GCAGCCCCAC
961CCTCCAGGCT GTCTCCACCC ATGTTAGAAG AAATGGCTTA TTCTGCCTAC GTGCCCCAAG
1021ATGGAACGAT GTTAACTGCG CTGCATAGTT ATATGGATGC TCATTCACCA ACAACTCTTG
1081GACTCCAGCC CTTGTTACCC CATTCAATGA CACAACTGCC AATAAGTCAT ACCTACCCAA
1141CTTTCTTGTA CAAAGTtggc attataagaa agcattgctt atcaatttgt tgcaacgaac

Download FASTA (ORF) (Full)