Construct: ORF ccsbBroadEn_10676

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF000019.1_s300c1, BRDN0000387470
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
AMT (275)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 275 AMT aminomethyltransferase NM_001164711.2 82.5% 81.2% (many diffs)
2 human 275 AMT aminomethyltransferase NM_001164712.2 74.8% 74.8% 1_291del
3 human 275 AMT aminomethyltransferase NM_000481.4 71% 69.9% (many diffs)
4 human 275 AMT aminomethyltransferase NM_001164710.2 70.7% 59% (many diffs)
5 human 275 AMT aminomethyltransferase NR_028435.2 43.3% (many diffs)
6 mouse 434437 Amt aminomethyltransferase NM_001013814.1 60.4% 62.7% (many diffs)
7 mouse 434437 Amt aminomethyltransferase XM_011242894.2 51.5% 53.9% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
933
ORF length:
867
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGGA GAGTCTAGTG GTTGGAGACA TTGCAGAGCT AAGACCAAAC CAGGGGACAC
121TGTCGCTGTT TACCAACGAG GCTGGAGGCA TCTTAGATGA CTTGATTGTA ACCAATACTT
181CTGAGGGCCA CCTGTATGTG GTGTCCAACG CTGGCTGCTG GGAGAAAGAT TTGGCCCTCA
241TGCAGGACAA GGTCAGGGAG CTTCAGAACC AGGGCAGAGA TGTGGGCCTG GAGGTGTTGG
301ATAATGCCCT GCTAGCTCTG CAAGGCCCCA CTGCAGCCCA GGTACTACAG GCCGGCGTGG
361CAGATGACCT GAGGAAACTG CCCTTCATGA CCAGTGCTGT GATGGAGGTG TTTGGCGTGT
421CTGGCTGCCG CGTGACCCGC TGTGGCTACA CAGGAGAGGA TGGTGTGGAG ATCTCGGTGC
481CGGTAGCGGG GGCAGTTCAC CTGGCAACAG CTATTCTGAA AAACCCAGAG GTGAAGCTGG
541CAGGGCTGGC AGCCAGGGAC AGCCTGCGCC TGGAGGCAGG CCTCTGCCTG TATGGGAATG
601ACATTGATGA ACACACTACA CCTGTGGAGG GCAGCCTCAG TTGGACACTG GGGAAGCGCC
661GCCGAGCTGC TATGGACTTC CCTGGAGCCA AGGTCATTGT TCCCCAGCTG AAGGGCAGGG
721TGCAGCGGAG GCGTGTGGGG TTGATGTGTG AGGGGGCCCC CATGCGGGCA CACAGTCCCA
781TCCTGAACAT GGAGGGTACC AAGATTGGTA CTGTGACTAG TGGCTGCCCC TCCCCCTCTC
841TGAAGAAGAA TGTGGCGATG GGTTATGTGC CCTGCGAGTA CAGTCGTCCA GGGACAATGC
901TGCTGGTAGA GCTTCCTTCA GGACCCTGCT TCTGCCCAAC TTTCTTGTAC AAAGTtggca
961ttataagaaa gcattgctta tcaatttgtt gcaacgaac

Download FASTA (ORF) (Full)