Construct: ORF ccsbBroadEn_10772
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005540.1_s300c1, BRDN0000390459
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DDX1 (1653)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1653 | DDX1 | DEAD-box helicase 1 | NM_004939.3 | 94.1% | 94.1% | 1_129del;912C>T |
| 2 | mouse | 104721 | Ddx1 | DEAD (Asp-Glu-Ala-Asp) box ... | NM_134040.1 | 84.8% | 91.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2157
- ORF length:
- 2091
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG 61 TTGGCATGGC TGCAGAAACA GGAAGTGGCA AAACTGGTGC TTTTAGTATT CCAGTTATCC 121 AGATAGTTTA TGAAACTCTG AAAGACCAAC AGGAAGGCAA AAAAGGAAAA ACAACAATTA 181 AAACTGGTGC TTCAGTGCTG AACAAATGGC AGATGAACCC ATATGACAGA GGATCTGCTT 241 TTGCAATTGG GTCAGATGGT CTTTGTTGTC AAAGCAGAGA AGTAAAGGAA TGGCATGGGT 301 GTAGAGCTAC TAAAGGATTA ATGAAAGGGA AACACTACTA TGAAGTATCC TGTCATGACC 361 AAGGGTTATG CAGGGTCGGG TGGTCTACCA TGCAGGCCTC TTTGGACCTA GGTACTGACA 421 AGTTTGGATT TGGCTTTGGT GGAACAGGAA AGAAATCCCA TAACAAACAA TTTGATAATT 481 ATGGAGAGGA ATTCACTATG CATGATACCA TTGGATGTTA CCTGGATATA GATAAGGGAC 541 ATGTCAAGTT CTCCAAAAAT GGAAAAGATC TTGGTCTGGC ATTTGAAATA CCACCACATA 601 TGAAAAACCA AGCCCTCTTT CCTGCCTGTG TTTTGAAGAA TGCTGAACTG AAATTTAACT 661 TCGGTGAAGA GGAATTTAAG TTTCCACCAA AAGATGGCTT TGTTGCTCTT TCCAAGGCAC 721 CGGATGGTTA CATTGTCAAA TCACAGCACT CAGGTAATGC ACAGGTGACA CAAACAAAGT 781 TTCTCCCCAA TGCTCCGAAA GCTCTCATTG TTGAACCTTC CCGGGAGTTA GCTGAACAAA 841 CTTTGAATAA CATCAAGCAG TTTAAGAAAT ACATTGATAA TCCTAAATTA AGGGAGCTTC 901 TGATAATTGG AGGTGTTGCA GCCCGGGATC AGCTCTCTGT TTTGGAAAAT GGAGTAGATA 961 TAGTTGTAGG TACTCCGGGA AGACTAGATG ACTTGGTGTC AACTGGAAAG CTGAACTTAT 1021 CTCAAGTTAG ATTCCTGGTC CTGGATGAAG CTGATGGGCT TCTTTCTCAA GGTTATTCTG 1081 ATTTTATAAA TAGGATGCAC AATCAGATTC CTCAGGTTAC CTCTGATGGA AAAAGACTTC 1141 AGGTGATTGT TTGCTCTGCC ACTTTGCATT CTTTCGATGT AAAGAAACTG TCCGAGAAGA 1201 TAATGCATTT TCCTACATGG GTTGACTTAA AAGGAGAAGA CTCTGTTCCA GATACTGTAC 1261 ACCATGTTGT TGTCCCAGTA AATCCCAAAA CTGACAGACT CTGGGAAAGG CTTGGAAAGA 1321 GCCACATTAG AACTGATGAT GTACATGCAA AAGATAACAC AAGACCTGGT GCTAATAGTC 1381 CAGAGATGTG GTCTGAAGCT ATTAAAATCC TGAAAGGGGA GTATGCTGTC CGGGCAATCA 1441 AGGAACATAA GATGGATCAA GCAATTATCT TCTGTAGAAC CAAAATTGAC TGTGATAACT 1501 TGGAGCAGTA CTTTATACAA CAAGGAGGAG GACCTGATAA AAAAGGACAC CAGTTCTCAT 1561 GTGTTTGTCT TCATGGTGAC AGAAAGCCTC ATGAGAGAAA GCAAAACTTG GAAAGATTTA 1621 AGAAAGGAGA TGTAAGATTC TTGATTTGCA CAGATGTAGC TGCTAGAGGA ATTGATATCC 1681 ACGGTGTTCC TTATGTTATA AATGTCACTC TGCCCGATGA AAAGCAAAAC TACGTACATC 1741 GAATTGGCAG AGTAGGAAGA GCTGAAAGGA TGGGTCTGGC AATTTCCCTG GTGGCAACAG 1801 AAAAAGAAAA GGTTTGGTAC CATGTATGTA GCAGCCGTGG AAAAGGGTGT TATAACACAA 1861 GACTCAAGGA AGATGGAGGC TGTACCATAT GGTACAACGA GATGCAGTTA CTATCTGAGA 1921 TAGAAGAACA CCTGAACTGT ACCATTTCTC AGGTTGAGCC GGATATAAAG GTACCAGTGG 1981 ATGAATTTGA TGGGAAAGTT ACCTACGGTC AGAAAAGGGC TGCTGGTGGT GGAAGCTATA 2041 AAGGCCATGT GGATATTTTG GCACCTACTG TTCAAGAGTT GGCTGCCCTT GAAAAGGAGG 2101 CGCAGACATC TTTCCTGCAT CTTGGCTACC TTCCTAACCA GCTGTTCAGA ACCTTCTGCC 2161 CAACTTTCTT GTACAAAGTt ggcattataa gaaagcattg cttatcaatt tgttgcaacg 2221 aac