Construct: ORF ccsbBroadEn_10811
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006889.1_s300c1, BRDN0000397594
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MECOM (2122)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2122 | MECOM | MDS1 and EVI1 complex locus | NM_001366473.2 | 16.5% | 14.4% | (many diffs) |
| 2 | human | 2122 | MECOM | MDS1 and EVI1 complex locus | XM_017005877.1 | 16.5% | 14.3% | (many diffs) |
| 3 | human | 2122 | MECOM | MDS1 and EVI1 complex locus | XM_005247225.4 | 16.4% | 14.2% | (many diffs) |
| 4 | human | 2122 | MECOM | MDS1 and EVI1 complex locus | XM_005247224.3 | 16.3% | 14.2% | (many diffs) |
| 5 | human | 2122 | MECOM | MDS1 and EVI1 complex locus | NM_001366466.2 | 12.2% | 10.6% | (many diffs) |
| 6 | human | 2122 | MECOM | MDS1 and EVI1 complex locus | XM_005247214.3 | 12.2% | 10.6% | (many diffs) |
| 7 | human | 2122 | MECOM | MDS1 and EVI1 complex locus | NM_004991.4 | 12.1% | 10.5% | (many diffs) |
| 8 | human | 2122 | MECOM | MDS1 and EVI1 complex locus | XM_005247213.3 | 12.1% | 10.5% | (many diffs) |
| 9 | mouse | 14013 | Mecom | MDS1 and EVI1 complex locus | NM_021442.2 | 66.2% | 65.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 573
- ORF length:
- 507
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG 61 TTGGCATGAG ATCCAAAGGC AGGGCAAGGA AACTGGCCAC AAATAATGAG TGTGTATATG 121 GCAACTACCC TGAAATACCT TTGGAAGAAA TGCCAGATGC AGATGGAGTA GCCAGCACTC 181 CCTCCCTCAA TATTCAAGAG CCATGCTCTC CTGCCACATC CAGTGAAGCA TTCACTCCAA 241 AGGAGGGTTC TCCTTACAAA GCCCCCATCT ACATCCCTGA TGATATCCCC ATTCCTGCTG 301 AGTTTGAACT TCGAGAGTCA AATATGCCTG GGGCAGGACT AGGAATATGG ACCAAAAGGA 361 AGATCGAAGT AGGTGAAAAG TTTGGGCCTT ATGTGGGAGA GCAGAGGTCA AACCTGAAAG 421 ACCCCAGTTA TGGATGGGAG GTACATCTTC CAAGGTCTCG GAGGGTAAGC GTTCACTCTT 481 GGTTGTATTT GGGGAAGAGA AGCTCAGACG TAGGAATAGC CTTCTCTCAG GCTGATGTCT 541 ACATGCCTGG ACTGCAGTGT GCCTTCCTCT CGTACCCAAC TTTCTTGTAC AAAGTtggca 601 ttataagaaa gcattgctta tcaatttgtt gcaacgaac