Construct: ORF ccsbBroadEn_10994

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF007288.1_s300c1, BRDN0000397710
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
NEK2 (4751)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 4751 NEK2 NIMA related kinase 2 NM_001204183.1 55.6% 55.2% 504T>C;639_652del;657_1152del
2 human 4751 NEK2 NIMA related kinase 2 NM_001204182.1 55% 54.6% 504T>C;639_652del;657_1164del
3 human 4751 NEK2 NIMA related kinase 2 XM_005273147.2 48.8% 48.5% 504T>C;639_652del;657_1311del
4 human 4751 NEK2 NIMA related kinase 2 NM_002497.4 48% 47.6% 504T>C;639_652del;657_1335del
5 mouse 18005 Nek2 NIMA (never in mitosis gene... XM_006497123.3 42.2% 45.6% (many diffs)
6 mouse 18005 Nek2 NIMA (never in mitosis gene... NM_010892.3 41.5% 44.9% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
708
ORF length:
642
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGCC TTCCCGGGCT GAGGACTATG AAGTGTTGTA CACCATTGGC ACAGGCTCCT
121ACGGCCGCTG CCAGAAGATC CGGAGGAAGA GTGATGGCAA GATATTAGTT TGGAAAGAAC
181TTGACTATGG CTCCATGACA GAAGCTGAGA AACAGATGCT TGTTTCTGAA GTGAATTTGC
241TTCGTGAACT GAAACATCCA AACATCGTTC GTTACTATGA TCGGATTATT GACCGGACCA
301ATACAACACT GTACATTGTA ATGGAATATT GTGAAGGAGG GGATCTGGCT AGTGTAATTA
361CAAAGGGAAC CAAGGAAAGG CAATACTTAG ATGAAGAGTT TGTTCTTCGA GTGATGACTC
421AGTTGACTCT GGCCCTGAAG GAATGCCACA GACGAAGTGA TGGTGGTCAT ACCGTATTGC
481ATCGGGATCT GAAACCAGCC AATGTTTTCC TGGATGGCAA GCAAAACGTC AAGCTTGGAG
541ACTTTGGGCT AGCTAGAATA TTAAACCACG ACACGAGTTT TGCAAAAACA TTTGTTGGCA
601CACCTTATTA CATGTCTCCT GAACAAATGA ATCGCATGTC CTACAATGAG AAATCAGATA
661TCTGGTCATT GGGCTGCTTG CTGTATGAGT TATGTGCATT AATCTTTTAC CCAACTTTCT
721TGTACAAAGT tggcattata agaaagcatt gcttatcaat ttgttgcaac gaac

Download FASTA (ORF) (Full)