Transcript: Mouse XM_006497123.3

PREDICTED: Mus musculus NIMA (never in mitosis gene a)-related expressed kinase 2 (Nek2), transcript variant X1, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Nek2 (18005)
Length:
3368
CDS:
366..1676

Additional Resources:

NCBI RefSeq record:
XM_006497123.3
NBCI Gene record:
Nek2 (18005)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006497123.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000274582 AGTTACTATGATCGCATTATT pLKO_005 570 CDS 100% 15.000 21.000 N Nek2 n/a
2 TRCN0000274617 ACCCACTAGAGATTGGTATTC pLKO_005 1717 3UTR 100% 10.800 8.640 N Nek2 n/a
3 TRCN0000027589 GCCTCCCTTTACAGCTTTCAA pLKO.1 1004 CDS 100% 5.625 3.938 N Nek2 n/a
4 TRCN0000027615 GTGTGGAAAGAGCTTGACTAT pLKO.1 468 CDS 100% 4.950 3.465 N Nek2 n/a
5 TRCN0000285711 GTGTGGAAAGAGCTTGACTAT pLKO_005 468 CDS 100% 4.950 3.465 N Nek2 n/a
6 TRCN0000027640 GACCTGAAGAAGAGGCTTCAT pLKO.1 1572 CDS 100% 0.495 0.347 N Nek2 n/a
7 TRCN0000285710 GACCTGAAGAAGAGGCTTCAT pLKO_005 1572 CDS 100% 0.495 0.347 N Nek2 n/a
8 TRCN0000027618 GCCATGAAGAAGAAGGTTCAT pLKO.1 1476 CDS 100% 0.495 0.347 N Nek2 n/a
9 TRCN0000274616 GCCATGAAGAAGAAGGTTCAT pLKO_005 1476 CDS 100% 0.495 0.347 N Nek2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006497123.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 TRCN0000489400 CTCGCCACCCATCAACGGCCTCCT pLX_317 27% 81.7% 86.7% V5 (not translated due to prior stop codon) (many diffs) n/a
2 TRCN0000489960 CCTTCGCCTCACGACCAAAATCTG pLX_317 30.8% 81.6% 86.5% V5 (many diffs) n/a
3 ccsbBroadEn_10994 pDONR223 100% 42.2% 45.6% None (many diffs) n/a
4 ccsbBroad304_10994 pLX_304 0% 42.2% 45.6% V5 (many diffs) n/a
5 TRCN0000469737 GAGCTGACTGCCTCCACTTGACAC pLX_317 56.6% 42.2% 45.6% V5 (many diffs) n/a
6 ccsbBroadEn_14710 pDONR223 0% 42.2% 45.6% None (many diffs) n/a
7 ccsbBroad304_14710 pLX_304 0% 42.2% 45.6% V5 (many diffs) n/a
8 TRCN0000468900 GCGGAGTCGCTCCTGGGTCCTTAG pLX_317 56.9% 42.2% 45.6% V5 (many diffs) n/a
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