Transcript: Human NM_002497.4

Homo sapiens NIMA related kinase 2 (NEK2), transcript variant 1, mRNA.

Source:
NCBI, updated 2019-09-27
Taxon:
Homo sapiens (human)
Gene:
NEK2 (4751)
Length:
2134
CDS:
143..1480

Additional Resources:

NCBI RefSeq record:
NM_002497.4
NBCI Gene record:
NEK2 (4751)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001145653 ACTATGATCGGATTATTGAC pXPR_003 CGG 225 17% 2 1.0492 NEK2 NEK2 76215
2 BRDN0001145920 GTGGTCATACCGTATTGCAT pXPR_003 CGG 414 31% 3 0.3703 NEK2 NEK2 76216
3 BRDN0001146193 GCCGCTGCCAGAAGATCCGG pXPR_003 AGG 75 6% 1 0.3304 NEK2 NEK2 76214
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_002497.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000000949 CGTTCGTTACTATGATCGGAT pLKO.1 343 CDS 100% 2.640 3.696 N NEK2 n/a
2 TRCN0000000950 CGTTACTCTGATGAATTGAAT pLKO.1 857 CDS 100% 5.625 4.500 N NEK2 n/a
3 TRCN0000314634 CGTTACTCTGATGAATTGAAT pLKO_005 857 CDS 100% 5.625 4.500 N NEK2 n/a
4 TRCN0000314711 CTTTGGGCTAGCTAGAATATT pLKO_005 619 CDS 100% 15.000 10.500 N NEK2 n/a
5 TRCN0000381529 TGAGAGAAGAGGGCGACAATT pLKO_005 991 CDS 100% 13.200 9.240 N NEK2 n/a
6 TRCN0000195248 CTGGCTAGTGTAATTACAAAG pLKO.1 422 CDS 100% 10.800 7.560 N NEK2 n/a
7 TRCN0000314637 TGATGGTGGTCATACCGTATT pLKO_005 535 CDS 100% 10.800 7.560 N NEK2 n/a
8 TRCN0000000948 GCCATGCCTTTCTGTATAGTA pLKO.1 1579 3UTR 100% 5.625 3.938 N NEK2 n/a
9 TRCN0000314709 GCCATGCCTTTCTGTATAGTA pLKO_005 1579 3UTR 100% 5.625 3.938 N NEK2 n/a
10 TRCN0000000952 CCTGTATTGAGTGAGCTGAAA pLKO.1 1043 CDS 100% 4.950 3.465 N NEK2 n/a
11 TRCN0000000951 GCAGACGAGCAAAGAAGAAAT pLKO.1 968 CDS 100% 13.200 7.920 N NEK2 n/a
12 TRCN0000314635 GCAGACGAGCAAAGAAGAAAT pLKO_005 968 CDS 100% 13.200 7.920 N NEK2 n/a
13 TRCN0000195573 CCAAGGAAAGGCAATACTTAG pLKO.1 447 CDS 100% 10.800 6.480 N NEK2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_002497.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 TRCN0000489400 CTCGCCACCCATCAACGGCCTCCT pLX_317 27% 99.7% 100% V5 (not translated due to prior stop codon) (many diffs) n/a
2 TRCN0000489960 CCTTCGCCTCACGACCAAAATCTG pLX_317 30.8% 99.6% 99.7% V5 (many diffs) n/a
3 ccsbBroadEn_10994 pDONR223 100% 48% 47.6% None 504T>C;639_652del;657_1335del n/a
4 ccsbBroad304_10994 pLX_304 0% 48% 47.6% V5 504T>C;639_652del;657_1335del n/a
5 TRCN0000469737 GAGCTGACTGCCTCCACTTGACAC pLX_317 56.6% 48% 47.6% V5 504T>C;639_652del;657_1335del n/a
6 ccsbBroadEn_14710 pDONR223 0% 48% 47.6% None 504T>C;639_652del;657_1335del n/a
7 ccsbBroad304_14710 pLX_304 0% 48% 47.6% V5 504T>C;639_652del;657_1335del n/a
8 TRCN0000468900 GCGGAGTCGCTCCTGGGTCCTTAG pLX_317 56.9% 48% 47.6% V5 504T>C;639_652del;657_1335del n/a
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