Construct: ORF ccsbBroadEn_10999
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003000.1_s300c1, BRDN0000457248
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NOP2 (4839)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4839 | NOP2 | NOP2 nucleolar protein | NM_001258309.2 | 100% | 100% | |
| 2 | human | 4839 | NOP2 | NOP2 nucleolar protein | NM_001258308.2 | 96% | 96% | 473_474ins99 |
| 3 | human | 4839 | NOP2 | NOP2 nucleolar protein | XM_005253691.2 | 96% | 96% | 473_474ins99 |
| 4 | human | 4839 | NOP2 | NOP2 nucleolar protein | NM_001033714.3 | 95.6% | 95.6% | 236_237insAGGGATCTCTGC;461_462ins99 |
| 5 | human | 4839 | NOP2 | NOP2 nucleolar protein | NM_006170.4 | 95.6% | 95.6% | 236_237insAGGGATCTCTGC;461_462ins99 |
| 6 | human | 4839 | NOP2 | NOP2 nucleolar protein | NM_001258310.2 | 72.7% | 69.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2604
- ORF length:
- 2535
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaacttTG TACAAAAAAG 61 TTGGCACCAT GGGGCGCAAG TTGGACCCTA CGAAGGAGAA GCGGGGGCCA GGCCGAAAGG 121 CCCGGAAGCA GAAGGGTGCC GAGACAGAAC TCGTCAGATT CTTGCCTGCA GTAAGTGACG 181 AAAATTCCAA GAGGCTGTCT AGTCGTGCTC GAAAGAGGGC AGCCAAGAGG AGATTGGGCT 241 CTGTTGAAGC CCCTAAGACA AATAAGTCTC CTGAGGCCAA ACCATTGCCT GGAAAGCTAC 301 CAAAAGGGAT CTCTGCAGGA GCTGTCCAGA CAGCTGGTAA GAAGGGACCC CAGTCCCTAT 361 TTAATGCTCC TCGAGGCAAG AAGCGCCCAG CACCTGGCAG TGATGAGGAA GAGGAGGAGG 421 AAGACTCTGA AGAAGATGGT ATGGTGAACC ACGGGGACCT CTGGGGCTCC GAGGACGATG 481 CTGATACGGT AGATGACTAT GGAGCTGACT CCAACTCTGA GGATGAGGAG GAAGGTGAAG 541 CGGCTGGAGT GCAGTGGCTC GGTCTTGGCT CACTGCAACC TCCACCTCCC GGGTTCAAGC 601 AATTCTCCTG CCTCAGCTTC CCAAGTAGCT GGGATTTACA GTTGCTGCCC ATTGAAAGAG 661 CTGCTCGGAA GCAGAAGGCC CGGGAAGCTG CTGCTGGGAT CCAGTGGAGT GAAGAGGAGA 721 CCGAGGACGA GGAGGAAGAG AAAGAAGTGA CCCCTGAGTC AGGCCCCCCA AAGGTGGAAG 781 AGGCAGATGG GGGCCTGCAG ATCAATGTGG ATGAGGAACC ATTTGTGCTG CCCCCTGCTG 841 GGGAGATGGA GCAGGATGCC CAGGCTCCAG ACCTGCAACG AGTTCACAAG CGGATCCAGG 901 ATATTGTGGG AATTCTGCGT GATTTTGGGG CTCAGCGGGA GGAAGGGCGG TCTCGTTCTG 961 AATACCTGAA CCGGCTCAAG AAGGATCTGG CCATTTACTA CTCCTATGGA GACTTCCTGC 1021 TTGGCAAGCT CATGGACCTC TTCCCTCTGT CTGAGCTGGT GGAGTTCTTA GAAGCTAATG 1081 AGGTGCCTCG GCCCGTCACC CTCCGGACCA ATACCTTGAA AACCCGACGC CGAGACCTTG 1141 CACAGGCTCT AATCAATCGT GGGGTTAACC TGGATCCCCT GGGCAAGTGG TCAAAGACTG 1201 GACTAGTGGT GTATGATTCT TCTGTGCCCA TTGGTGCTAC CCCCGAGTAC CTGGCTGGGC 1261 ACTACATGCT GCAGGGAGCC TCCAGCATGT TGCCCGTCAT GGCCTTGGCA CCCCAGGAAC 1321 ATGAGCGGAT CCTGGACATG TGTTGTGCCC CTGGAGGAAA GACCAGCTAC ATGGCCCAGC 1381 TGATGAAGAA CACGGGTGTG ATCCTTGCCA ATGACGCCAA TGCTGAGCGG CTCAAGAGTG 1441 TTGTGGGCAA CTTGCATCGG CTGGGAGTCA CCAACACCAT TATCAGCCAC TATGATGGGC 1501 GCCAGTTCCC CAAGGTGGTG GGGGGCTTTG ACCGAGTACT GCTGGATGCT CCCTGCAGTG 1561 GCACTGGGGT CATCTCCAAG GATCCAGCCG TGAAGACTAA CAAGGATGAG AAGGACATCC 1621 TGCGCTGTGC TCACCTCCAG AAGGAGTTGC TCCTGAGTGC TATTGACTCT GTCAATGCGA 1681 CCTCCAAGAC AGGAGGCTAC CTGGTTTACT GCACCTGTTC TATCACAGTA GAAGAGAATG 1741 AGTGGGTGGT AGACTATGCT CTGAAAAAGA GGAATGTGCG ACTGGTGCCC ACGGGCCTAG 1801 ACTTTGGCCA GGAAGGTTTT ACCCGCTTTC GAGAAAGGCG CTTCCACCCC AGTCTGCGTT 1861 CTACCCGACG CTTCTACCCT CATACCCACA ATATGGATGG GTTCTTCATT GCCAAGTTCA 1921 AGAAATTTTC CAATTCTATC CCTCAGTCCC AGACAGGAAA TTCTGAAACA GCCACACCTA 1981 CAAATGTAGA CTTGCCTCAG GTCATCCCCA AGTCTGAGAA CAGCAGCCAG CCAGCCAAGA 2041 AAGCCAAGGG GGCTGCAAAG ACAAAGCAGC AGCTGCAGAA ACAGCAACAT CCCAAGAAGG 2101 CCTCCTTCCA GAAGCTGAAT GGCATCTCCA AAGGGGCAGA CTCAGAATTG TCCACTGTAC 2161 CTTCTGTCAC AAAGACCCAA GCTTCCTCCA GCTTCCAGGA TAGCAGTCAG CCAGCTGGAA 2221 AAGCCGAAGG GATCAGGGAG CCAAAGGTGA CTGGGAAGCT AAAGCAACGA TCACCTAAAT 2281 TACAGTCCTC CAAGAAAGTT GCTTTCCTCA GGCAGAATGC CCCTCCCAAG GGCACAGACA 2341 CACAAACACC GGCTGTGTTA TCCCCATCCA AGACTCAGGC CACCCTGAAA CCTAAGGACC 2401 ATCATCAGCC CCTTGGAAGG GCCAAGGGGG TTGAGAAGCA GCAGTTGCCA GAGCAGCCTT 2461 TTGAGAAAGC TGCCTTCCAG AAACAGAATG ATACCCCCAA GGGGCCTCAG CCTCCCACTG 2521 TGTCTCCCAT CCGTTCCAGC CGCCCCCCAC CAGCAAAGAG GAAGAAATCT CAGTCCAGGG 2581 GCAACAGCCA GCTGCTGCTA TCTTTGCCAA CTTTCTTGTA CAAAGTTGGc attataagaa 2641 agcattgctt atcaatttgt tgcaacgaac