Transcript: Human NM_001258310.2

Homo sapiens NOP2 nucleolar protein (NOP2), transcript variant 5, mRNA.

Source:
NCBI, updated 2019-06-02
Taxon:
Homo sapiens (human)
Gene:
NOP2 (4839)
Length:
2760
CDS:
90..1976

Additional Resources:

NCBI RefSeq record:
NM_001258310.2
NBCI Gene record:
NOP2 (4839)

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001258310.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000157484 GACGATGCTGATACGGTAGAT pLKO.1 483 CDS 100% 4.950 6.930 N NOP2 n/a
2 TRCN0000285513 GACGATGCTGATACGGTAGAT pLKO_005 483 CDS 100% 4.950 6.930 N NOP2 n/a
3 TRCN0000153307 GAGTGCTATTGACTCTGTCAA pLKO.1 1565 CDS 100% 4.950 6.930 N NOP2 n/a
4 TRCN0000154296 GCTAAAGCAACGATCACCTAA pLKO.1 2290 3UTR 100% 4.950 6.930 N NOP2 n/a
5 TRCN0000157117 GCCGTGAAGACTAACAAGGAT pLKO.1 1497 CDS 100% 3.000 4.200 N NOP2 n/a
6 TRCN0000285510 GAAACAGCCACACCTACAAAT pLKO_005 1997 3UTR 100% 13.200 9.240 N NOP2 n/a
7 TRCN0000285509 ATGAGTGGGTGGTAGACTATG pLKO_005 1648 CDS 100% 10.800 7.560 N NOP2 n/a
8 TRCN0000153019 GCCTTCCAGAAACAGAATGAT pLKO.1 2504 3UTR 100% 5.625 3.938 N NOP2 n/a
9 TRCN0000276269 GCCTTCCAGAAACAGAATGAT pLKO_005 2504 3UTR 100% 5.625 3.938 N NOP2 n/a
10 TRCN0000152472 CACTGTACCTTCTGTCACAAA pLKO.1 2185 3UTR 100% 4.950 3.465 N NOP2 n/a
11 TRCN0000276268 CACTGTACCTTCTGTCACAAA pLKO_005 2185 3UTR 100% 4.950 3.465 N NOP2 n/a
12 TRCN0000157241 CCACTGTACCTTCTGTCACAA pLKO.1 2184 3UTR 100% 4.950 3.465 N NOP2 n/a
13 TRCN0000157909 CGAGACAGAACTCGTCAGATT pLKO.1 161 CDS 100% 4.950 3.465 N NOP2 n/a
14 TRCN0000152792 GCCATTTACTACTCCTATGGA pLKO.1 900 CDS 100% 3.000 2.100 N NOP2 n/a
15 TRCN0000151506 CCTAAGACAAATAAGTCTCCT pLKO.1 273 CDS 100% 2.640 1.848 N NOP2 n/a
16 TRCN0000152560 GTCCTCCAAGAAAGTTGCTTT pLKO.1 2317 3UTR 100% 4.950 2.970 N NOP2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001258310.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_15509 pDONR223 0% 76% 72.5% None (many diffs) n/a
2 ccsbBroad304_15509 pLX_304 0% 76% 72.5% V5 (many diffs) n/a
3 TRCN0000474625 TGCTCAACTGTCATAATATGTCTT pLX_317 12.5% 76% 72.5% V5 (many diffs) n/a
4 ccsbBroadEn_10999 pDONR223 100% 72.7% 69.4% None (many diffs) n/a
5 ccsbBroad304_10999 pLX_304 0% 72.7% 69.4% V5 (many diffs) n/a
6 TRCN0000476171 AGTTTGAAAGAATACATCCCAGTA pLX_317 13.2% 72.7% 69.4% V5 (many diffs) n/a
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