Construct: ORF ccsbBroadEn_11243
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001745.1_s300c1, BRDN0000455855
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NPHS2 (7827)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7827 | NPHS2 | NPHS2 stomatin family membe... | NM_001297575.2 | 99.6% | 100% | 102A>G;288C>T;834A>G |
| 2 | human | 7827 | NPHS2 | NPHS2 stomatin family membe... | XM_017002298.1 | 86% | 80% | (many diffs) |
| 3 | human | 7827 | NPHS2 | NPHS2 stomatin family membe... | NM_014625.4 | 81.9% | 82.2% | (many diffs) |
| 4 | human | 7827 | NPHS2 | NPHS2 stomatin family membe... | XM_005245483.3 | 78.5% | 66.8% | (many diffs) |
| 5 | human | 7827 | NPHS2 | NPHS2 stomatin family membe... | XM_017002299.1 | 56.2% | 56.5% | 102A>G;288C>T;534_535ins411 |
| 6 | mouse | 170484 | Nphs2 | nephrosis 2, podocin | NM_130456.4 | 69.8% | 69.3% | (many diffs) |
| 7 | mouse | 170484 | Nphs2 | nephrosis 2, podocin | XM_006496684.3 | 49.4% | 40.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1011
- ORF length:
- 945
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAGAAAG 61 TTGGCATGGA GAGGAGGGCG CGGAGCTCCT CCAGGGAGTC CCGCGGGCGA GGCGGCAGGA 121 CTCCGCACAA GGAGAACAAG AGGGCAAAGG CCGAGAGGAG CGGCGGGGGC CGCGGGCGCC 181 AGGAGGCTGG GCCCGAGCCG TCGGGCTCCG GACGGGCGGG GACCCCGGGG GAGCCCCGAG 241 CGCCCGCCGC CACGGTGGTG GACGTGGATG AGGTCCGAGG CTCCGGCGAG GAGGGCACCG 301 AGGTGGTGGC GCTGTTGGAG AGCGAGCGGC CCGAGGAAGG TACCAAATCC TCTGGCTTAG 361 GGGCCTGTGA GTGGCTTCTT GTCCTCATTT CCCTGCTCTT CATCATCATG ACCTTCCCTT 421 TTTCCATCTG GTTCTGCGTA AAGGTTGTAC AAGAGTATGA AAGAGTAATT ATATTCCGAC 481 TGGGACATCT GCTTCCTGGA AGAGCCAAAG GCCCTGGTCT TTTCTTTTTT TTGCCCTGCC 541 TGGATACCTA CCACAAGGTT GACCTTCGTC TCCAAACTCT GGAGATACCT TTTCATGAGG 601 TTGCCTTGGA TTCAGTGACC TGTATTTGGG GAATCAAAGT GGAGAGAATA GAAATTAAAG 661 ATGTGAGGTT GCCAGCTGGG CTTCAGCACT CACTGGCTGT GGAGGCTGAA GCGCAAAGAC 721 AAGCCAAAGT GCGGATGATT GCTGCAGAAG CGGAAAAGGC TGCTTCTGAG TCCCTGAGGA 781 TGGCAGCTGA GATTCTGTCA GGCACCCCTG CTGCCGTTCA GCTTCGATAC CTCCACACCC 841 TTCAGTCTCT GTCCACAGAG AAGCCTTCCA CTGTGGTTTT ACCTTTGCCA TTTGACCTGC 901 TGAATTGCCT GTCTTCTCCC AGCAACAGAA CTCAGGGAAG CCTCCCCTTC CCAAGTCCTT 961 CCAAACCTGT TGAGCCACTA AATCCTAAAA AGAAAGACTC TCCCATGTTA TACCCAACTT 1021 TCTTGTACAA AGTTGGcatt ataagaaagc attgcttatc aatttgttgc aacgaac