Construct: ORF ccsbBroadEn_11284
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005838.1_s300c1, BRDN0000385481
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- USO1 (8615)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8615 | USO1 | USO1 vesicle transport factor | NM_003715.3 | 99.8% | 99.8% | 277_279delGAA;1563T>C;1986G>A |
| 2 | human | 8615 | USO1 | USO1 vesicle transport factor | XM_006714397.3 | 99.3% | 99.3% | (many diffs) |
| 3 | human | 8615 | USO1 | USO1 vesicle transport factor | XM_006714396.4 | 99.1% | 99.1% | (many diffs) |
| 4 | human | 8615 | USO1 | USO1 vesicle transport factor | NM_001290049.1 | 98.6% | 98.6% | (many diffs) |
| 5 | mouse | 56041 | Uso1 | USO1 vesicle docking factor | NM_019490.1 | 86.1% | 92.3% | (many diffs) |
| 6 | mouse | 56041 | Uso1 | USO1 vesicle docking factor | XM_006535140.3 | 85.5% | 91.6% | (many diffs) |
| 7 | mouse | 56041 | Uso1 | USO1 vesicle docking factor | XM_017321009.1 | 83.6% | 90% | (many diffs) |
| 8 | mouse | 56041 | Uso1 | USO1 vesicle docking factor | XM_006535141.3 | 83% | 89.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2949
- ORF length:
- 2883
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG 61 TTGGCATGAA TTTCCTCCGC GGGGTAATGG GGGGTCAGAG TGCCGGACCC CAGCACACAG 121 AAGCCGAGAC GATTCAAAAG CTTTGTGACA GAGTAGCTTC ATCTACTTTA TTGGATGATC 181 GAAGAAATGC TGTTCGTGCT CTCAAATCAT TATCTAAGAA ATACCGCTTG GAAGTGGGTA 241 TACAAGCTAT GGAACATCTT ATTCATGTTT TACAAACAGA TCGTTCAGAT TCTGAAATAA 301 TAGGTTATGC TTTGGACACA CTATATAATA TAATATCTAA TGAAGAAGAA GAAGTAGAAG 361 AAAATTCCAC AAGACAGAGT GAAGATTTGG GAAGCCAATT TACAGAAATT TTCATTAAGC 421 AGCAGGAAAA TGTCACTCTT CTGTTATCTT TATTGGAGGA GTTTGATTTC CATGTCCGCT 481 GGCCTGGTGT GAAGCTTCTT ACTTCTCTTT TAAAACAACT AGGGCCTCAG GTGCAACAAA 541 TTATTTTAGT CAGTCCTATG GGTGTTTCAA GATTGATGGA CTTACTAGCG GATTCCAGGG 601 AAGTTATACG TAATGATGGC GTCTTACTAC TGCAGGCACT AACAAGAAGC AATGGTGCAA 661 TCCAGAAAAT TGTTGCTTTT GAAAATGCTT TCGAGAGACT ACTGGACATT ATTTCAGAGG 721 AGGGGAACAG TGATGGAGGT ATAGTAGTTG AAGATTGTTT GATTTTGCTC CAAAACTTAT 781 TAAAAAACAA CAACTCCAAT CAAAATTTTT TTAAAGAAGG CTCATATATT CAACGTATGA 841 AACCTTGGTT TGAAGTTGGA GATGAAAATT CTGGCTGGTC TGCACAGAAA GTGACCAATC 901 TACATCTAAT GCTACAGCTT GTTCGTGTAT TGGTATCTCC CACCAACCCT CCTGGTGCTA 961 CCAGTAGCTG CCAGAAGGCT ATGTTCCAGT GTGGGTTATT GCAGCAGCTT TGTACTATCC 1021 TAATGGCTAC TGGGGTTCCT GCTGATATCC TGACTGAGAC CATAAATACT GTATCAGAAG 1081 TTATTCGAGG CTGCCAAGTA AACCAAGACT ACTTTGCATC TGTAAATGCA CCTTCAAACC 1141 CACCAAGACC GGCAATTGTA GTACTTCTCA TGTCCATGGT TAATGAAAGG CAGCCATTTG 1201 TTTTGCGCTG TGCTGTTCTC TATTGTTTCC AGTGTTTCTT GTATAAAAAC CAAAAAGGAC 1261 AAGGAGAAAT CGTGTCAACA CTTTTACCTT CTACCATTGA TGCAACAGGT AATTCAGTTT 1321 CAGCTGGCCA GTTATTATGT GGAGGTTTGT TTTCTACTGA TTCACTTTCA AACTGGTGTG 1381 CTGCTGTGGC CCTTGCCCAT GCGTTGCAAG AAAATGCCAC CCAGAAAGAA CAGTTGCTCA 1441 GGGTTCAACT TGCTACAAGT ATTGGCAACC CTCCAGTTTC TTTACTTCAA CAGTGCACCA 1501 ATATTCTTTC ACAGGGAAGC AAAATACAAA CAAGAGTTGG ATTATTAATG TTGCTTTGTA 1561 CCTGGCTAAG CAATTGTCCC ATTGCAGTAA CGCATTTTCT TCACAATTCA GCCAATGTTC 1621 CATTCCTTAC AGGACAAATT GCAGAAAATC TTGGAGAAGA AGAGCAGTTG GTCCAAGGCT 1681 TATGTGCCCT TTTGTTGGGC ATTTCGATTT ATTTCAATGA TAACTCACTT GAGAGCTACA 1741 TGAAAGAGAA GCTAAAACAA CTGATTGAGA AGAGGATTGG CAAAGAGAAT TTCATAGAGA 1801 AACTAGGATT TATTAGCAAA CATGAGTTGT ATTCCAGAGC ATCTCAGAAA CCCCAGCCAA 1861 ACTTTCCCAG TCCAGAATAC ATGATATTTG ATCATGAGTT TACGAAGCTG GTAAAAGAAC 1921 TTGAAGGTGT TATAACTAAG GCTATTTATA AGTCCAGTGA AGAAGATAAA AAAGAAGAAG 1981 AGGTGAAAAA AACATTAGAA CAGCATGACA ATATTGTGAC TCACTACAAA AATATGATTC 2041 GAGAGCAAGA TCTCCAACTT GAGGAATTAA GGCAGCAGGT TTCTACATTA AAATGTCAAA 2101 ATGAACAGCT CCAGACGGCA GTCACACAGC AAGTATCACA GATCCAGCAG CACAAAGACC 2161 AGTATAATCT TCTTAAAATA CAGCTAGGAA AAGACAATCA GCATCAAGGT TCTTACAGTG 2221 AGGGGGCTCA GATGAATGGC ATTCAGCCAG AAGAAATTGG TAGATTGCGA GAAGAGATAG 2281 AAGAATTAAA ACGTAATCAG GAACTTTTAC AAAGCCAGCT GACTGAAAAG GACTCTATGA 2341 TTGAAAATAT GAAATCTTCC CAAACATCTG GCACAAATGA ACAGTCTTCA GCAATAGTTT 2401 CAGCTAGAGA TTCTGAACAA GTTGCAGAAT TAAAACAGGA ACTGGCAACT TTAAAGTCTC 2461 AGTTAAACTC ACAATCTGTG GAGATCACCA AACTACAGAC AGAAAAGCAG GAACTGTTAC 2521 AGAAAACAGA AGCGTTTGCA AAATCAGTTG AGGTACAAGG AGAGACCGAG ACTATAATAG 2581 CCACCAAAAC TACTGATGTA GAAGGAAGAC TGTCAGCATT ATTACAAGAG ACCAAAGAGT 2641 TAAAGAATGA AATTAAAGCT CTGTCTGAGG AAAGAACTGC CATTAAAGAG CAGCTGGATT 2701 CATCTAATAG TACCATTGCC ATTTTACAAA CTGAGAAAGA CAAACTAGAG TTGGAAATTA 2761 CAGATTCTAA AAAAGAACAA GATGATCTCT TGGTGCTCTT GGCCGATCAA GATCAGAAAA 2821 TACTGTCATT GAAGAATAAA CTCAAGGATC TTGGTCATCC AGTTGAAGAA GAGGATGAAC 2881 TTGAATCTGG AGACCAAGAG GATGAGGATG ATGAAAGTGA AGATCCTGGC AAGGATCTAG 2941 ATCATATCTA CCCAACTTTC TTGTACAAAG Ttggcattat aagaaagcat tgcttatcaa 3001 tttgttgcaa cgaac