Transcript: Human XM_006714397.3

PREDICTED: Homo sapiens USO1 vesicle transport factor (USO1), transcript variant X2, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
USO1 (8615)
Length:
4117
CDS:
299..3199

Additional Resources:

NCBI RefSeq record:
XM_006714397.3
NBCI Gene record:
USO1 (8615)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_006714397.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000420135 TGTTGGGCATTTCGATTTATT pLKO_005 1941 CDS 100% 15.000 21.000 N USO1 n/a
2 TRCN0000412582 TGAGTCCTTAGTTGATGATTT pLKO_005 3602 3UTR 100% 13.200 18.480 N USO1 n/a
3 TRCN0000065070 GCAGCTTTGTACTATCCTAAT pLKO.1 1252 CDS 100% 10.800 15.120 N USO1 n/a
4 TRCN0000420719 AGGAACAATAGAGATTATATC pLKO_005 3213 3UTR 100% 13.200 9.240 N USO1 n/a
5 TRCN0000427118 ATGTTCCAGTGTGGGTTATTG pLKO_005 1229 CDS 100% 13.200 9.240 N USO1 n/a
6 TRCN0000454972 CCATTGATGCAACAGGTAATT pLKO_005 1542 CDS 100% 13.200 9.240 N USO1 n/a
7 TRCN0000413725 CCTTAGTTCATTCGTTGTTTA pLKO_005 3496 3UTR 100% 13.200 9.240 N USO1 n/a
8 TRCN0000412714 GTTCGTGCTCTCAAATCATTA pLKO_005 425 CDS 100% 13.200 9.240 N USO1 n/a
9 TRCN0000065072 GCACAGAAAGTGACCAATCTA pLKO.1 1130 CDS 100% 5.625 3.938 N USO1 n/a
10 TRCN0000065068 CCAGTTATTATGTGGAGGTTT pLKO.1 1576 CDS 100% 4.950 3.465 N USO1 n/a
11 TRCN0000065069 GCATTATTACAAGAGACCAAA pLKO.1 2864 CDS 100% 4.950 3.465 N USO1 n/a
12 TRCN0000065071 GCTACATGAAAGAGAAGCTAA pLKO.1 1983 CDS 100% 4.950 3.465 N USO1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006714397.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_11284 pDONR223 100% 99.3% 99.3% None (many diffs) n/a
2 ccsbBroad304_11284 pLX_304 0% 99.3% 99.3% V5 (many diffs) n/a
3 TRCN0000478906 GCCTACTCATTTACTGCACACCAA pLX_317 14.3% 99.3% 99.3% V5 (many diffs) n/a
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