Construct: ORF ccsbBroadEn_11416
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013980.1_s300c1, BRDN0000393317
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CKAP5 (9793)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9793 | CKAP5 | cytoskeleton associated pro... | NM_001008938.4 | 46.8% | 46.8% | 1_3237del;4297A>G |
| 2 | human | 9793 | CKAP5 | cytoskeleton associated pro... | NM_014756.3 | 43.9% | 43.8% | 1_3237del;4297A>G;4688_4689ins180 |
| 3 | mouse | 75786 | Ckap5 | cytoskeleton associated pro... | NM_001165989.1 | 42.5% | 44.7% | (many diffs) |
| 4 | mouse | 75786 | Ckap5 | cytoskeleton associated pro... | XM_006500344.3 | 42.5% | 44.7% | (many diffs) |
| 5 | mouse | 75786 | Ckap5 | cytoskeleton associated pro... | XM_006500342.1 | 42.3% | 44.5% | (many diffs) |
| 6 | mouse | 75786 | Ckap5 | cytoskeleton associated pro... | XM_006500343.2 | 42.3% | 44.5% | (many diffs) |
| 7 | mouse | 75786 | Ckap5 | cytoskeleton associated pro... | NM_029437.2 | 41.5% | 43.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2925
- ORF length:
- 2859
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG 61 TTGGCATGCC AGCCAAGCCT GCTCCACCCA CTAAAGCAAC TTCTAAACCA ATGGGAGGGT 121 CCGCTCCAGC CAAATTCCAG CCTGCATCAG CACCTGCTGA AGATTGTATT TCCAGCAGTA 181 CAGAACCCAA ACCTGATCCA AAAAAGGCCA AAGCTCCAGG ATTATCCTCT AAAGCAAAGA 241 GTGCACAAGG GAAGAAGATG CCAAGCAAAA CCAGCTTAAA GGAGGATGAA GACAAATCCG 301 GGCCTATTTT TATTGTTGTT CCAAATGGAA AAGAGCAAAG GATGAAAGAT GAAAAAGGAT 361 TGAAGGTGCT AAAGTGGAAT TTTACTACCC CACGGGATGA ATACATTGAG CAACTAAAGA 421 CTCAAATGTC TAGCTGTGTG GCTAAATGGT TACAAGATGA GATGTTTCAC TCAGACTTTC 481 AGCATCATAA CAAAGCCCTT GCTGTTATGG TTGATCACTT GGAGAGTGAA AAAGAAGGAG 541 TTATTGGTTG CCTGGATCTT ATCTTAAAGT GGCTTACCCT GAGGTTTTTT GACACCAATA 601 CAAGCGTCCT GATGAAAGCA CTAGAATATT TAAAATTGCT CTTCACCTTG CTAAGTGAAG 661 AAGAATATCA TCTTACTGAG AATGAAGCAT CTTCCTTCAT CCCCTATCTT GTCGTCAAGG 721 TTGGAGAACC AAAGGATGTC ATTCGTAAAG ATGTTCGTGC CATCCTGAAC CGGATGTGCC 781 TTGTCTACCC AGCTAGCAAG ATGTTTCCCT TTATCATGGA AGGAACCAAA TCCAAAAACT 841 CTAAGCAGAG AGCAGAGTGC CTGGAAGAGC TGGGATGTCT GGTTGAGTCC TATGGCATGA 901 ATGTTTGCCA ACCAACCCCA GGAAAAGCCT TAAAGGAAAT AGCTGTTCAC ATAGGAGACC 961 GTGACAATGC TGTACGCAAT GCTGCACTCA ACACCATTGT AACGGTGTAC AATGTACATG 1021 GGGATCAGGT GTTCAAACTG ATTGGAAATC TTTCTGAAAA GGATATGAGC ATGCTCGAGG 1081 AGAGGATTAA GCGGTCAGCA AAGAGACCCT CTGCTGCACC AATAGAACAG GTGGAAGAGA 1141 AACCTCAGCG TGCACAGAAC ATAAGCTCCA ATGCCAACAT GTTACGCAAG GGACCAGCTG 1201 AGGACATGTC TTCCAAACTC AACCAAGCCC GAAGCATGAG TGGGCATCCT GAGGCAGCCC 1261 AGATGGTCCG CCGAGAATTC CAGCTGGATC TAGATGAGAT TGAGAATGAC AATGGTACAG 1321 TCCGATGTGA AATGCCAGAA CTTGTTCAGC ACAAACTGGA TGACATTTTT GAGCCAGTCC 1381 TTATTCCTGA ACCCAAGATC CGGGCTGTTT CTCCACACTT CGATGACATG CACAGTAATA 1441 CAGCATCCAC AATCAATTTC ATTATCTCCC AAGTAGCCAG TGGTGACATC AACACAAGTA 1501 TCCAAGCTCT GACACAGATC GATGAGGTCC TGAGACAGGA AGACAAAGCT GAAGCCATGT 1561 CCGGCCATAT TGATCAGTTT CTGATAGCCA CTTTTATGCA GCTAAGACTC ATCTACAACA 1621 CACACATGGC AGATGAGAAA TTGGAGAAGG ACGAGATCAT CAAGTTGTAT AGCTGTATCA 1681 TTGGCAACAT GATTTCGCTG TTTCAGATAG AGAGCCTTGC CCGGGAGGCC TCCACTGGAG 1741 TACTAAAAGA CCTAATGCAT GGCCTCATCA CCTTAATGCT GGATTCTCGG ATTGAAGATC 1801 TTGAGGAAGG ACAACAGGTC ATCCGCTCTG TGAACCTCTT GGTGGTGAAG GTTCTGGAGA 1861 AGTCAGACCA GACCAACATC CTGAGTGCCC TACTTGTTTT GCTCCAAGAC AGCCTGCTAG 1921 CAACAGCCAG TTCTCCCAAA TTCTCAGAGC TTGTTATGAA GTGTCTCTGG AGAATGGTTC 1981 GACTGTTGCC TGATACCATC AATAGCATTA ACCTAGACAG AATTCTTCTG GATATCCACA 2041 TTTTCATGAA GGTCTTCCCC AAAGAGAAAC TGAAGCAATG CAAAAGTGAA TTTCCCATAA 2101 GGACCCTAAA GACCCTGCTA CACACCTTAT GCAAATTAAA AGGGCCCAAG ATCCTGGACC 2161 ACCTAACGAT GATCGACAAC AAAAACGAGT CTGAGCTGGA GGCCCATCTC TGCCGGATGA 2221 TGAAGCACAG TATGGACCAG ACTGGGAGCA AGTCTGATAA GGAAACAGAA AAGGGAGCAT 2281 CTCGAATAGA TGAAAAATCA TCAAAGGCCA AAGTGAATGA TTTCTTAGCT GAGATTTTTA 2341 AGAAGATTGG CTCTAAAGAA AACACTAAAG AGGGACTAGC AGAGTTATAT GAATATAAGA 2401 AGAAATACTC AGATGCTGAC ATTGAACCAT TTCTGAAAAA TTCCTCACAG TTCTTCCAGA 2461 GCTATGTCGA AAGAGGCCTT CGGGTGATTG AGATGGAGAG GGAGGGCAAA GGTCGTATTT 2521 CCACTTCAAC AGGCATCTCC CCTCAGATGG AAGTCACATG TGTGCCCACG CCCACAAGCA 2581 CAGTGTCCTC CATAGGTAAC ACAAATGGGG AAGAAGTGGG GCCATCTGTC TACTTGGAAA 2641 GGCTAAAGAT CCTCCGACAG CGATGTGGTC TGGACAACAC AAAGCAAGAT GACCGACCTC 2701 CTTTGACCTC TTTGCTCTCC AAACCAGCAG TTCCTACTGT CGCCTCTTCC ACAGACATGC 2761 TCCACAGCAA ACTCTCTCAG CTCCGGGAGT CACGGGAGCA GCACCAGCAT TCAGACCTGG 2821 ATTCTAACCA GACTCACTCT TCAGGAACTG TGACCTCCTC CTCCTCCACA GCTAACATAG 2881 ACGACTTGAA AAAAAGACTG GAGAGAATAA AGAGCAGTCG CAAATGCCCA ACTTTCTTGT 2941 ACAAAGTtgg cattataaga aagcattgct tatcaatttg ttgcaacgaa c