Construct: ORF ccsbBroadEn_11637
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009957.1_s300c1, BRDN0000390703
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SEC31A (22872)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 22872 | SEC31A | SEC31 homolog A, COPII coat... | NM_001300744.2 | 47.2% | 47.1% | (many diffs) |
| 2 | human | 22872 | SEC31A | SEC31 homolog A, COPII coat... | NM_001077206.3 | 45.7% | 45.4% | (many diffs) |
| 3 | human | 22872 | SEC31A | SEC31 homolog A, COPII coat... | NM_001300745.2 | 43.2% | 43.1% | (many diffs) |
| 4 | human | 22872 | SEC31A | SEC31 homolog A, COPII coat... | NM_016211.4 | 42.7% | 42.6% | (many diffs) |
| 5 | human | 22872 | SEC31A | SEC31 homolog A, COPII coat... | NM_001077208.3 | 42% | 41.7% | (many diffs) |
| 6 | human | 22872 | SEC31A | SEC31 homolog A, COPII coat... | NM_001318119.1 | 42% | 41.7% | (many diffs) |
| 7 | human | 22872 | SEC31A | SEC31 homolog A, COPII coat... | NM_001318120.1 | 41.5% | 41.2% | (many diffs) |
| 8 | human | 22872 | SEC31A | SEC31 homolog A, COPII coat... | NM_001077207.4 | 41.4% | 41.2% | (many diffs) |
| 9 | human | 22872 | SEC31A | SEC31 homolog A, COPII coat... | NM_001191049.2 | 40.9% | 40% | (many diffs) |
| 10 | mouse | 69162 | Sec31a | Sec31 homolog A (S. cerevis... | XM_006535207.3 | 48.8% | 52.2% | (many diffs) |
| 11 | mouse | 69162 | Sec31a | Sec31 homolog A (S. cerevis... | XM_011249563.2 | 42% | 45.1% | (many diffs) |
| 12 | mouse | 69162 | Sec31a | Sec31 homolog A (S. cerevis... | XM_011249562.2 | 40.6% | 43.3% | (many diffs) |
| 13 | mouse | 69162 | Sec31a | Sec31 homolog A (S. cerevis... | XM_011249561.2 | 39.4% | 42.2% | (many diffs) |
| 14 | mouse | 69162 | Sec31a | Sec31 homolog A (S. cerevis... | XM_011249560.2 | 38.5% | 41.4% | (many diffs) |
| 15 | mouse | 69162 | Sec31a | Sec31 homolog A (S. cerevis... | XM_011249559.2 | 38% | 40.8% | (many diffs) |
| 16 | mouse | 69162 | Sec31a | Sec31 homolog A (S. cerevis... | XM_011249558.2 | 37.6% | 40.4% | (many diffs) |
| 17 | mouse | 69162 | Sec31a | Sec31 homolog A (S. cerevis... | XM_011249557.2 | 37.3% | 39.9% | (many diffs) |
| 18 | mouse | 69162 | Sec31a | Sec31 homolog A (S. cerevis... | XM_017321089.1 | 37.3% | 39.9% | (many diffs) |
| 19 | mouse | 69162 | Sec31a | Sec31 homolog A (S. cerevis... | XM_011249556.2 | 36.8% | 39.4% | (many diffs) |
| 20 | mouse | 69162 | Sec31a | Sec31 homolog A (S. cerevis... | XM_017321088.1 | 36.8% | 39.4% | (many diffs) |
| 21 | mouse | 69162 | Sec31a | Sec31 homolog A (S. cerevis... | XM_011249555.2 | 36.6% | 39.4% | (many diffs) |
| 22 | mouse | 69162 | Sec31a | Sec31 homolog A (S. cerevis... | NM_026969.1 | 36.4% | 38.9% | (many diffs) |
| 23 | mouse | 69162 | Sec31a | Sec31 homolog A (S. cerevis... | XM_011249554.2 | 36.3% | 38.8% | (many diffs) |
| 24 | mouse | 69162 | Sec31a | Sec31 homolog A (S. cerevis... | XM_011249553.2 | 36.3% | 38.8% | (many diffs) |
| 25 | mouse | 69162 | Sec31a | Sec31 homolog A (S. cerevis... | XM_011249552.2 | 36% | 38.4% | (many diffs) |
| 26 | mouse | 69162 | Sec31a | Sec31 homolog A (S. cerevis... | XM_011249551.2 | 35.9% | 38.4% | (many diffs) |
| 27 | mouse | 69162 | Sec31a | Sec31 homolog A (S. cerevis... | XM_006535192.2 | 35.5% | 38% | (many diffs) |
| 28 | mouse | 69162 | Sec31a | Sec31 homolog A (S. cerevis... | XM_006535193.1 | 35.5% | 38% | (many diffs) |
| 29 | mouse | 69162 | Sec31a | Sec31 homolog A (S. cerevis... | XM_006535194.2 | 35.5% | 38% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1593
- ORF length:
- 1527
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG 61 TTGGCATGAA GTTAAAGGAA GTAGATCGTA CAGCCATGCA GGCATGGAGC CCTGCCCAGA 121 ATCACCCCAT TTACCTAGCA ACAGGAACAT CTGCTCAGCA ATTGGATGCA ACATTTAGTA 181 CGAATGCTTC CCTTGAGATA TTTGAATTAG ACCTCTCTGA TCCATCCTTG GATATGAAAT 241 CTTGTGCCAC ATTCTCCTCT TCTCACAGGT ACCACAAGTT GATTTGGGGG CCTTATAAAA 301 TGGATTCCAA AGGAGATGTC TCTGGAGTTC TGATTGCAGG TGGTGAAAAT GGAAATATTA 361 TTCTCTATGA TCCTTCTAAA ATTATAGCTG GAGACAAGGA AGTTGTGATT GCCCAGAATG 421 ACAAGCATAC TGGCCCAGTG AGAGCCTTGG ATGTGAACAT TTTCCAGACT AATCTGGTAG 481 CTTCTGGTGC TAATGAATCT GAAATCTACA TATGGGATCT AAATAATTTT GCAACCCCAA 541 TGACACCAGG AGCCAAAACA CAGCCGCCAG AAGATATCAG CTGCATTGCA TGGAACAGAC 601 AAGTTCAGCA TATTTTAGCA TCAGCCAGTC CCAGTGGCCG GGCCACTGTA TGGGATCTTA 661 GAAAAAATGA GCCAATCATC AAAGTCAGTG ACCATAGTAA CAGAATGCAT TGTTCTGGGT 721 TGGCATGGCA TCCTGATGTT GCTACTCAGA TGGTCCTTGC CTCCGAGGAT GACCGGTTAC 781 CAGTGATCCA GATGTGGGAT CTTCGATTTG CTTCCTCTCC ACTTCGTGTC CTGGAAAACC 841 ATGCCAGGGG GATTTTGGCA ATTGCTTGGA GCATGGCAGA TCCTGAATTG TTACTGAGCT 901 GTGGAAAAGA TGCTAAGATT CTCTGCTCCA ATCCAAACAC AGGAGAGGTG TTATATGAAC 961 TTCCCACCAA CACACAGTGG TGCTTCGATA TTCAGTGGTG TCCCCGAAAT CCTGCTGTCT 1021 TATCAGCTGC TTCGTTTGAT GGGCGTATCA GTGTTTATTC TATCATGGGA GGAAGCACAG 1081 ATGGTTTAAG ACAGAAACAA GTTGACAAGC TTTCATCATC TTTTGGGAAT CTTGATCCCT 1141 TTGGCACAGG ACAGCCCCTT CCTCCGTTAC AAATTCCACA GCAGACTGCT CAGCATAGTA 1201 TAGTGCTGCC TCTGAAGAAG CCGCCCAAGT GGATTCGAAG GCCTGTTGGT GCTTCTTTTT 1261 CATTTGGAGG CAAACTGGTT ACGTTTGAGA ATGTCAGAAT GCCTTCTCAT CAGGGAGCTG 1321 AGCAGCAGCA GCAGCAGCAC CATGTGTTCA TTAGTCAGGT TGTAACAGAA AAGGAGTTCC 1381 TCAGCCGATC AGACCAACTT CAGCAGGCTG TGCAGTCACA AGGATTTATC AATTATTGCC 1441 AAAAAAAAAT TGATGCTTCT CAGACTGAAT TTGAGAAAAA TGTGTGGTCC TTTTTGAAGG 1501 TAAACTTTGA GGATGATTCT CGTGGAAAAT ACCTTGAACT TCTAGGATAC AGAAAAGAAG 1561 ATCTAGGAAA GAAGGTAAAC TTTTGGGAAA GTTACCCAAC TTTCTTGTAC AAAGTtggca 1621 ttataagaaa gcattgctta tcaatttgtt gcaacgaac