Construct: ORF ccsbBroadEn_11653

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF009959.1_s300c1, BRDN0000388264
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
RPIA (22934)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 22934 RPIA ribose 5-phosphate isomerase A NM_144563.3 76.2% 76.2% 1_222del
2 mouse 19895 Rpia ribose 5-phosphate isomerase A NM_009075.2 70.7% 73.3% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
777
ORF length:
711
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAA
61TTGGCATGTC CAAGGCCGAG GAGGCCAAGA AGCTGGCGGG CCGCGCGGCT GTGGAGAACC
121ACGTGAGGAA TAACCAAGTG CTGGGAATTG GAAGTGGTTC TACAATTGTC CATGCTGTGC
181AGCGAATAGC TGAAAGGGTG AAGCAAGAGA ATCTGAACCT CGTCTGTATT CCCACTTCCT
241TCCAGGCCCG CCAGCTCATC CTGCAGTATG GCTTGACCCT CAGTGATCTG GATCGACACC
301CAGAGATCGA CCTTGCCATC GATGGTGCTG ATGAAGTAGA TGCTGATCTC AATCTCATCA
361AGGGTGGCGG AGGCTGCCTG ACCCAGGAGA AGATTGTGGC TGGCTATGCT AGTCGCTTCA
421TCGTGATCGC TGATTTCAGG AAAGATTCGA AGAATCTCGG GGATCAGTGG CACAAGGGAA
481TCCCCATCGA GGTCATCCCA ATGGCCTATG TCCCAGTGAG CCGAGCTGTG AGCCAGAAGT
541TTGGGGGCGT GGTTGAACTT CGAATGGCTG TCAACAAGGC TGGTCCTGTG GTGACAGATA
601ATGGGAATTT TATCTTGGAC TGGAAGTTTG ACCGGGTACA CAAATGGAGT GAAGTGAATA
661CAGCTATCAA AATGATCCCA GGTGTGGTGG ACACAGGCCT ATTCATCAAC ATGGCTGAGA
721GAGTCTACTT TGGGATGCAG GATGGCTCAG TGAACATGAG GGAGAAGCCT TTCTGTTGCC
781CAACTTTCTT GTACAAAGTt ggcattataa gaaagcattg cttatcaatt tgttgcaacg
841aac

Download FASTA (ORF) (Full)