Construct: ORF ccsbBroadEn_11682

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF012529.1_s300c1, BRDN0000390972
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
RAD54L2 (23132)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 23132 RAD54L2 RAD54 like 2 NM_001322253.2 92.6% 92.5% 1_324del;745C>T
2 human 23132 RAD54L2 RAD54 like 2 NM_001322256.2 92.6% 92.5% 1_324del;745C>T
3 human 23132 RAD54L2 RAD54 like 2 NM_015106.4 92.6% 92.5% 1_324del;745C>T
4 human 23132 RAD54L2 RAD54 like 2 NM_001322257.1 74.3% 75.2% 0_1ins982;32_110del;1123_1124insGGCTAG
5 mouse 81000 Rad54l2 RAD54 like 2 (S. cerevisiae) NM_030730.2 84.4% 88.9% (many diffs)
6 mouse 81000 Rad54l2 RAD54 like 2 (S. cerevisiae) XM_017313702.1 53.7% 55.7% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
4143
ORF length:
4077
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGAG AAGAAACATA CGAAAGCTAC TCCGGGAGGA TCAATTGGAG CCTGTTACCA
121AAGCAGCACA GCAAGAAGAG TTGGAAAGAA GGAAGCGCCT GGAGCAGCAG AGGAAAGATT
181ATGCAGCCCC TATTCCTACT GTTCCGCTGG AGTTCCTCCC TGAGGAAATT GCTTTAAGGG
241CAAGTGACGG TCCCCAACTG CCTCCTCGGG TCTTGGCCCA GGAAGTCATT TGTTTGGACA
301GTAGCAGTGG CAGTGAGGAT GAAAAAAGCA GTCGAGATGA GGTGATTGAA CTGAGCTCTG
361GAGAGGAGGA CACTCTGCAC ATTGTGGACA GCAGTGAATC TGTCAGTGAA GATGATGAGG
421AAGAAGAGAA GGGTGGCACC CATGTCAATG ATGTCTTAAA CCAGCGTGAC GCCCTTGGGC
481GGGTCTTTGT CAACCTAAAC CACCCTCCAG AGGAGGAAAA TGTCTTCCTT GCCCCACAGT
541TGGCACGGGC TGTGAAACCT CATCAGATTG GCGGGATCCG GTTCCTTTAC GATAACCTAG
601TGGAGTCTCT GGAGAGGTTT AAGACCAGCA GTGGCTTTGG CTGTATTCTG GCCCACAGCA
661TGGGTCTGGG GAAAACTTTG CAAGTGATCT CTTTCATCGA CGTCCTCTTC CGCCACACGC
721CAGCCAAAAC AGTCCTTGCC ATTGTGCCGG TTAATACTCT TCAGAATTGG CTGGCAGAGT
781TCAACATGTG GCTTCCACCT CCTGAAGCCC TCCCGGCTGA CAACAAGCCT GAAGAAGTCC
841AGCCTCGGTT CTTTAAAGTT CACATCTTGA ATGATGAGCA CAAGACGATG GCATCTCGTG
901CTAAAGTGAT GGCTGATTGG GTGTCAGAGG GTGGCGTGCT GCTGATGGGG TACGAGATGT
961ACAGACTCCT CACTCTGAAG AAATCATTTG CCACAGGTAG ACCGAAGAAA ACCAAGAAGC
1021GTTCTCACCC AGTCATCATT GATCTAGATG AGGAAGATCG GCAGCAGGAG TTTCGGAGAG
1081AGTTTGAGAA GGCTTTATGC CGCCCTGGCC CTGATGTAGT AATCTGTGAT GAGGGACACC
1141GCATCAAAAA CTGCCAGGCC AGCACCTCAC AGGCTCTGAA GAATATCCGC TCTCGCCGCC
1201GGGTGGTGCT GACTGGGTAC CCTCTGCAAA ACAACCTCAT TGAGTACTGG TGCATGGTGG
1261ACTTTGTGCG CCCAGACTTC CTTGGCACCC GGCAGGAGTT CAGCAACATG TTTGAACGCC
1321CTATCCTGAA TGGGCAATGT ATTGACAGCA CACCTCAGGA CGTCCGCCTC ATGCGGTACC
1381GGAGCCATGT CCTGCACAGT CTTCTGGAGG GCTTTGTGCA GAGGAGAGGC CACACTGTGC
1441TGAAGATTCA TCTCCCTGCC AAGGAAGAAA ATGTGATCCT TGTGCGGCTC TCCAAGATCC
1501AGCGAGATTT GTATACACAG TTCATGGATC GCTTCCGGGA CTGTGGTAGC AGCGGTTGGC
1561TGGGGCTGAA CCCCCTTAAG GCATTCTGTG TGTGTTGCAA GATCTGGAAT CACCCTGATG
1621TGCTGTATGA AGCCCTTCAG AAGGAGAGCT TGGCCAATGA GCAGGACCTA GACGTGGAAG
1681AACTTGGCTC TGCAGGGACC AGTGCCCGCT GTCCACCACA GGGAACAAAA GGCAAGGGAG
1741AGGATAGCAC CTTGGCTTCC TCGATGGGAG AGGCAACCAA TAGCAAGTTC CTACAGGGCG
1801TTGGCTTCAA CCCTTTCCAG GAGCGAGGCA ACAACATTGT CACATATGAA TGGGCCAAGG
1861ACCTTCTGAC TAATTACCAG ACTGGAGTCC TAGAAAACTC TCCCAAGATG GTACTGCTTT
1921TCCACCTGAT TGAGGAAAGT GTGAAGCTTG GGGACAAGAT CCTTGTGTTT AGCCAGAGTC
1981TTTCCACCTT GGCTCTCATC GAGGAATTCC TTGGAAAACG AGAAGTACCC TGTCCACCTG
2041GTACCGAGGG GCAAGGAGCA CAGAAGTGGG TTCGAAACAT CAGCTACTTC CGGCTAGATG
2101GTAGCACCCC TGCCTTTGAG AGGGAGCGGC TTATTAATCA GTTCAATGAT CCCAGCAACC
2161TCACCACCTG GCTGTTCCTT CTCTCTACAA GGGCCGGATG CTTGGGTGTG AATCTGATTG
2221GTGCCAACCG AGTGGTGGTG TTTGATGCTT CCTGGAACCC TTGCCATGAT GCCCAGGCAG
2281TATGTCGGGT ATACCGTTAT GGCCAGAAAA AGCCCTGTTA CATCTATCGC CTTGTGGCTG
2341ATTACACTCT AGAAAAGAAG ATCTATGACC GTCAGATTTC CAAGCAGGGC ATGTCAGATC
2401GGGTGGTGGA TGATCTAAAT CCAATGCTGA ACTTCACACG GAAAGAGGTG GAAAACCTAC
2461TGCACTTTGT TGAGAAGGAG CCAGCTCCCC AAGTTTCCTT GAACGTAAAG GGGATCAAGG
2521AGTCAGTCCT GCAACTGGCC TGTCTGAAGT ACCCTCACCT CATCACCAAG GAGCCTTTCG
2581AGCATGAGTC ATTGCTCTTG AACCGAAAGG ATCACAAGCT AACCAAGGCT GAGAAAAAAG
2641CAGCAAAGAA AAGCTATGAG GAAGACAAAC GCACATCAGT CCCCTATACC CGCCCATCGT
2701ATGCGCAGTA TTACCCTGCC AGCGATCAGA GCCTGACCAG CATCCCCGCC TTCAGCCAGA
2761GAAACTGGCA GCCAACTTTG AAGGGTGATG AAAAGCCTGT GGCCAGTGTT CGTCCTGTGC
2821AGTCCACCCC CATCCCCATG ATGCCCCGGC ATGTCCCATT GGGAGGAAGT GTAAGCTCTG
2881CCTCCAGCAC AAATCCATCC ATGAACTTTC CCATCAACTA CTTGCAGCGT GCAGGAGTCC
2941TTGTGCAGAA GGTGGTCACC ACGACAGATA TTGTTATTCC TGGACTCAAC AGCTCCACAG
3001ATGTACAGGC AAGAATTAAT GCTGGTGAGA GCATCCACAT CATCCGTGGG ACAAAAGGGA
3061CGTACATCCG TACCAGTGAT GGACGGATCT TTGCTGTCCG GGCAACTGGC AAACCAAAGG
3121TTCCTGAAGA TGGTCGGATG GCTGCCTCAG GTTCCCAGGG ACCTTCTTGC GAGTCCACAA
3181GCAACGGCAG ACACAGTGCC TCATCACCCA AAGCCCCCGA CCCTGAGGGG CTGGCCAGGC
3241CCGTCTCTCC TGACAGCCCA GAGATCATCA GTGAGCTTCA GCAGTATGCA GATGTGGCTG
3301CTGCCCGGGA ATCCCGTCAG AGCTCCCCAA GCACCAATGC CGCCCTGCCT GGCCCCCCGG
3361CCCAACTTAT GGACAGCAGT GCTGTTCCCG GGACAGCTCT CGGAACTGAG CCTCGACTAG
3421GGGGTCATTG CCTCAATAGT TCCCTCTTGG TGACTGGCCA GCCCTGTGGT GACAGGCACC
3481CAGTGCTGGA CTTAAGGGGC CACAAGCGAA AGTTGGCCAC ACCACCTGCT GCCCAGGAGT
3541CATCCCGCCG GCGGTCCAGG AAGGGTCATC TGCCAGCCCC CGTGCAGCCG TATGAACACG
3601GGTATCCAGT CTCTGGCGGG TTTGCCATGC CACCCGTCTC CTTAAACCAT AACCTCACCA
3661CCCCCTTCAC CTCCCAGGCT GGGGAGAACT CCCTGTTTAT GGGCAGTACC CCCTCCTACT
3721ACCAGCTGTC CAATTTGCTG GCAGATGCCC GCCTGGTGTT TCCAGTGACT ACTGACCCTC
3781TGGTGCCAGC AGGCCCCGTC AGTTCCTCTT CCACGGCTAC CTCAGTCACT GCCAGCAACC
3841CCTCCTTCAT GCTCAACCCT TCTGTGCCAG GGATACTACC CAGCTATTCA CTCCCATTCT
3901CACAGCCACT CCTGTCCGAG CCGAGGATGT TTGCGCCTTT TCCTTCCCCT GTCTTGCCCA
3961GCAACCTTTC GCGGGGCATG TCTATCTATC CAGGCTACAT GTCCCCACAT GCAGGCTACC
4021CAGCTGGTGG CCTCCTACGG TCCCAGGTGC CTCCATTTGA CTCTCATGAG GTTGCCGAGG
4081TTGGGTTCAG CTCCAATGAT GATGAGGATA AAGACGATGA TGTGATAGAG GTCACTGGGA
4141AATACCCAAC TTTCTTGTAC AAAGTtggca ttataagaaa gcattgctta tcaatttgtt
4201gcaacgaac

Download FASTA (ORF) (Full)