Construct: ORF ccsbBroadEn_11892

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF017761.1_s300c1, BRDN0000395773
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
MACROD1 (28992)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 28992 MACROD1 mono-ADP ribosylhydrolase 1 NM_014067.4 74.7% 74.7% 1_246del
2 human 28992 MACROD1 mono-ADP ribosylhydrolase 1 XM_005273939.4 72.6% 72.4% 1_246del;974A>C;976_1002del
3 human 28992 MACROD1 mono-ADP ribosylhydrolase 1 XM_011544970.2 69.6% 69.4% (many diffs)
4 human 28992 MACROD1 mono-ADP ribosylhydrolase 1 XM_006718521.3 32.4% 30.8% (many diffs)
5 human 28992 MACROD1 mono-ADP ribosylhydrolase 1 XM_005273940.2 30.5% 28.6% (many diffs)
6 human 28992 MACROD1 mono-ADP ribosylhydrolase 1 XM_006718522.2 27.9% 27.6% 1_246del;517_519delGGA;522_523ins456
7 mouse 107227 Macrod1 MACRO domain containing 1 NM_134147.4 65.6% 68.7% (many diffs)
8 mouse 107227 Macrod1 MACRO domain containing 1 XM_006526562.2 63.6% 66.1% (many diffs)
9 mouse 107227 Macrod1 MACRO domain containing 1 XM_006526563.2 60.9% 63.1% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
795
ORF length:
729
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGGC GGCGAAGGTG GACCTGAGCA CCTCCACCGA CTGGAAGGAG GCGAAATCCT
121TTCTGAAGGG CCTGAGTGAC AAGCAGCGGG AGGAACATTA CTTCTGCAAG GACTTTGTCA
181GGCTGAAGAA GATCCCGACA TGGAAGGAGA TGGCGAAAGG GGTGGCTGTG AAGGTGGAGG
241AGCCCAGGTA TAAAAAGGAC AAGCAGCTCA ATGAGAAAAT CTCCCTGCTC CGCAGCGACA
301TCACCAAGCT GGAGGTGGAC GCCATCGTCA ACGCCGCCAA CAGCTCCCTG CTCGGAGGCG
361GTGGCGTGGA CGGCTGCATT CATCGGGCCG CCGGCCCCCT GCTTACCGAC GAGTGCCGGA
421CCCTGCAGAG CTGTAAGACT GGCAAGGCCA AGATCACCGG CGGCTATCGG CTCCCGGCCA
481AGTACGTCAT CCACACAGTG GGGCCCATCG CCTACGGGGA GCCCAGCGCC AGTCAGGCTG
541CCGAGCTCCG CAGCTGCTAC CTGAGCAGTC TGGACCTGCT GCTGGAGCAC CGGCTCCGCT
601CGGTGGCGTT CCCCTGCATC TCCACCGGCG TGTTTGGCTA CCCCTGTGAG GCGGCCGCCG
661AGATCGTGCT GGCCACGCTG CGAGAGTGGC TGGAGCAGCA CAAGGACAAG GTGGACCGGC
721TGATCATCTG CGTGTTCCTC GAGAAGGACG AGGACATCTA CCGGAGCCGG CTCCCCCACT
781ACTTCCCCGT GGCCTGCCCA ACTTTCTTGT ACAAAGTtgg cattataaga aagcattgct
841tatcaatttg ttgcaacgaa c

Download FASTA (ORF) (Full)