Construct: ORF ccsbBroadEn_11963

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF004765.1_s300c1, BRDN0000388217
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
NUSAP1 (51203)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 51203 NUSAP1 nucleolar and spindle assoc... XM_017022295.1 91.4% 91.4% 1_60del;74_76delAGC
2 human 51203 NUSAP1 nucleolar and spindle assoc... XM_005254430.5 52.9% 52.9% 1_600del
3 human 51203 NUSAP1 nucleolar and spindle assoc... NM_001243143.2 52.8% 52.8% 1_600del;614_616delAGC
4 human 51203 NUSAP1 nucleolar and spindle assoc... XM_006720561.3 52% 52% 1_618del;632_634delAGC
5 human 51203 NUSAP1 nucleolar and spindle assoc... NM_001243142.2 51.2% 51.2% 1_642del
6 human 51203 NUSAP1 nucleolar and spindle assoc... NM_001301136.2 51.1% 51.1% 1_642del;656_658delAGC
7 human 51203 NUSAP1 nucleolar and spindle assoc... NM_018454.8 51.1% 51.1% 1_645del
8 human 51203 NUSAP1 nucleolar and spindle assoc... NM_016359.5 51% 51% 1_645del;659_661delAGC
9 human 51203 NUSAP1 nucleolar and spindle assoc... XM_005254428.3 50.4% 50.4% 1_663del
10 human 51203 NUSAP1 nucleolar and spindle assoc... XM_006720560.4 50.4% 50.4% 1_660del;674_676delAGC
11 human 51203 NUSAP1 nucleolar and spindle assoc... XM_006720559.3 50.3% 50.3% 1_663del;677_679delAGC
12 human 51203 NUSAP1 nucleolar and spindle assoc... NM_001243144.2 44.6% 44.6% 1_576del;933_934ins117
13 human 51203 NUSAP1 nucleolar and spindle assoc... XM_017022294.2 43.7% 43.7% 1_600del;957_958ins117
14 human 51203 NUSAP1 nucleolar and spindle assoc... XM_006720563.3 42.2% 42.2% 1_645del;1002_1003ins117
15 human 51203 NUSAP1 nucleolar and spindle assoc... XM_005254431.3 41.7% 41.7% 1_663del;1020_1021ins117
16 human 51203 NUSAP1 nucleolar and spindle assoc... XM_006720562.3 41.6% 41.6% 1_663del;677_679delAGC;1023_1024ins117
17 human 51203 NUSAP1 nucleolar and spindle assoc... XR_001751300.2 26.9% (many diffs)
18 human 51203 NUSAP1 nucleolar and spindle assoc... XR_001751299.2 26.4% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
741
ORF length:
675
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGAA TGAACTGAAG CAGCCCATCA ATAAGGGAGG GGTCAGGACT CCAGTACCTC
121CAAGAGGAAG ACTCTCTGTG GCTTCTACTC CCATCAGCCA ACGACGCTCG CAAGGCCGGT
181CTTGTGGCCC TGCAAGTCAG AGTACCTTGG GTCTGAAGGG GTCACTCAAG CGCTCTGCTA
241TCTCTGCAGC TAAAACGGGT GTCAGGTTTT CAGCTGCTAC TAAAGATAAT GAGCATAAGC
301GTTCACTGAC CAAGACTCCA GCCAGAAAGT CTGCACATGT GACCGTGTCT GGGGGCACCC
361CAAAAGGCGA GGCTGTGCTT GGGACACACA AATTAAAGAC CATCACGGGG AATTCTGCTG
421CTGTTATTAC CCCATTCAAG TTGACAACTG AGGCAACGCA GACTCCAGTC TCCAATAAGA
481AACCAGTGTT TGATCTTAAA GCAAGTTTGT CTCGTCCCCT CAACTATGAA CCACACAAAG
541GAAAGCTAAA ACCATGGGGG CAATCTAAAG AAAATAATTA TCTAAATCAA CATGTCAACA
601GAATTAACTT CTACAAGAAA ACTTACAAAC AACCCCATCT CCAGACAAAG GAAGAGCAAC
661GGAAGAAACG CGAGCAAGAA CGAAAGGAGA AGAAAGCAAA GGTTTTGGGA ATGCGAAGGG
721GCCTCATTTT GGCTGAAGAT TACCCAACTT TCTTGTACAA AGTtggcatt ataagaaagc
781attgcttatc aatttgttgc aacgaac

Download FASTA (ORF) (Full)