Construct: ORF ccsbBroadEn_12022
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004509.1_s300c1, BRDN0000396591
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NUP54 (53371)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 53371 | NUP54 | nucleoporin 54 | NM_017426.4 | 97.2% | 97.2% | 1480_1521del |
| 2 | human | 53371 | NUP54 | nucleoporin 54 | XM_011532033.3 | 90.1% | 90.1% | 1056_1057ins108;1372_1413del |
| 3 | human | 53371 | NUP54 | nucleoporin 54 | NM_001278603.1 | 87.7% | 87.5% | 151_152ins144;1336_1377del |
| 4 | human | 53371 | NUP54 | nucleoporin 54 | XR_001741237.2 | 62.2% | 1_60del;967_968ins55;1485_2231del | |
| 5 | human | 53371 | NUP54 | nucleoporin 54 | XM_017008295.2 | 61.7% | 61.7% | 0_1ins540;940_981del |
| 6 | human | 53371 | NUP54 | nucleoporin 54 | XR_001741238.2 | 57.5% | (many diffs) | |
| 7 | human | 53371 | NUP54 | nucleoporin 54 | NR_103780.1 | 52.5% | 1_141del;436_437ins227;1394_2154del | |
| 8 | human | 53371 | NUP54 | nucleoporin 54 | NR_103781.1 | 48% | (many diffs) | |
| 9 | human | 53371 | NUP54 | nucleoporin 54 | NR_103782.1 | 41.4% | (many diffs) | |
| 10 | mouse | 269113 | Nup54 | nucleoporin 54 | NM_183392.2 | 85% | 92.9% | (many diffs) |
| 11 | mouse | 269113 | Nup54 | nucleoporin 54 | XM_006534956.3 | 76.8% | 84% | (many diffs) |
| 12 | mouse | 269113 | Nup54 | nucleoporin 54 | XM_006534958.3 | 54.7% | 59.7% | (many diffs) |
| 13 | mouse | 269113 | Nup54 | nucleoporin 54 | XM_006534959.3 | 51.8% | 51.8% | (many diffs) |
| 14 | mouse | 269113 | Nup54 | nucleoporin 54 | XM_017320895.1 | 51.8% | 51.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1545
- ORF length:
- 1479
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG 61 TTGGCATGGC CTTCAATTTT GGGGCTCCCT CGGGCACCTC CGGTACCGCT GCAGCCACCG 121 CGGCCCCCGC GGGTGGGTTT GGAGGATTTG GGACAACATC TACAACTGCA GGTTCTGCAT 181 TCAGCTTTTC TGCCCCAACT AACACAGGCA CTACTGGACT CTTTGGTGGT ACTCAGAACA 241 AAGGTTTTGG ATTTGGTACT GGTTTTGGCA CAACAACGGG AACTAGTACT GGTTTAGGTA 301 CTGGTTTGGG AACTGGACTG GGATTTGGAG GATTTAATAC ACAGCAGCAG CAGCAAACTA 361 CATTAGGTGG TCTCTTCAGT CAGCCTACAC AAGCTCCTAC CCAGTCCAAC CAGCTGATAA 421 ATACTGCGAG TGCTCTTTCT GCTCCAACGC TGTTGGGAGA TGAGAGAGAT GCTATTTTGG 481 CAAAATGGAA TCAACTGCAG GCCTTTTGGG GAACAGGAAA AGGGTATTTC AACAATAATA 541 TTCCGCCAGT GGAATTCACA CAAGAAAATC CCTTTTGCCG ATTTAAGGCA GTAGGTTATA 601 GTTGCATGCC CAGTAATAAA GATGAAGATG GGCTAGTGGT TTTAGTTTTC AACAAAAAAG 661 AAACAGAGAT TCGAAGCCAA CAACAACAGT TGGTAGAATC ATTGCATAAA GTTTTGGGAG 721 GAAACCAGAC CCTTACTGTA AATGTAGAGG GCACTAAAAC ATTGCCAGAT GATCAGACAG 781 AAGTTGTTAT TTATGTTGTT GAGCGTTCGC CAAATGGTAC TTCAAGAAGA GTTCCAGCTA 841 CAACGCTATA TGCCCATTTT GAACAAGCCA ATATAAAAAC ACAATTGCAG CAACTTGGTG 901 TAACCCTTTC TATGACTAGA ACAGAACTTT CTCCTGCACA GATCAAACAG CTTTTACAGA 961 ATCCTCCTGC TGGTGTTGAT CCTATTATCT GGGAACAGGC CAAGGTAGAT AACCCTGATT 1021 CTGAAAAGTT AATTCCTGTA CCAATGGTGG GTTTTAAGGA ACTTCTCCGA AGACTGAAGG 1081 TTCAAGATCA GATGACTAAG CAGCATCAAA CCAGATTAGA TATCATATCT GAAGATATTA 1141 GTGAGCTACA AAAGAATCAA ACTACATCTG TAGCCAAAAT TGCACAATAC AAGAGGAAAC 1201 TCATGGATCT TTCCCATAGA ACTTTACAGG TCCTAATCAA ACAGGAAATT CAAAGGAAGA 1261 GTGGTTATGC CATTCAGGCT GATGAAGAGC AGTTGCGAGT TCAGCTGGAT ACGATTCAGG 1321 GTGAACTAAA TGCACCTACT CAGTTCAAGG GCCGACTAAA TGAATTGATG TCTCAAATCA 1381 GGATGCAGAA TCATTTTGGA GCAGTCAGAT CTGAAGAAAG GTATTACATA GATGCAGATC 1441 TGTTACGAGA AATCAAGCAG CATTTGAAAC AACAACAGGA AGGCCTTAGC CATTTGATTA 1501 GCATCATTAA AGACGATCTA GAAGATATAA AGCTGGTCGA ACATTGCCCA ACTTTCTTGT 1561 ACAAAGTtgg cattataaga aagcattgct tatcaatttg ttgcaacgaa c