Construct: ORF ccsbBroadEn_12070

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF018226.1_s300c1, BRDN0000389446
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
WDR74 (54663)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 54663 WDR74 WD repeat domain 74 NM_001307977.1 99.9% 100% 501G>T
2 human 54663 WDR74 WD repeat domain 74 NM_001369450.1 94.9% 95% 501G>T;920_976del
3 human 54663 WDR74 WD repeat domain 74 NM_001369451.1 94.9% 95% 501G>T;920_976del
4 human 54663 WDR74 WD repeat domain 74 NM_001369453.1 94.9% 95% 501G>T;920_976del
5 human 54663 WDR74 WD repeat domain 74 NM_018093.3 94.9% 95% 501G>T;920_976del
6 human 54663 WDR74 WD repeat domain 74 NM_001369447.1 91.6% 91.7% 370_411del;543G>T;962_1018del
7 human 54663 WDR74 WD repeat domain 74 NR_161381.1 89.7% (many diffs)
8 human 54663 WDR74 WD repeat domain 74 NM_001369448.1 59.9% 60% 0_1ins405;96G>T;515_571del
9 human 54663 WDR74 WD repeat domain 74 NM_001369449.1 59.9% 60% 0_1ins405;96G>T;515_571del
10 mouse 107071 Wdr74 WD repeat domain 74 NM_134139.1 81.6% 84.4% (many diffs)
11 mouse 107071 Wdr74 WD repeat domain 74 XM_006526554.3 52.5% 55.3% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1164
ORF length:
1098
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGGC GGCTGCTGCT GCACGCTGGA ACCATGTGTG GGTCGGCACC GAGACTGGGA
121TCTTGAAAGG GGTAAATCTT CAGCGAAAAC AGGCGGCGAA CTTCACGGCC GGAGGACAGC
181CGCGGCGCGA GGAGGCAGTG AGCGCCCTGT GTTGGGGCAC CGGCGGCGAG ACCCAGATGC
241TGGTGGGCTG CGCGGACAGG ACGGTGAAGC ACTTCAGCAC CGAGGATGGC ATATTCCAGG
301GTCAGAGACA CTGCCCGGGC GGGGAGGGCA TGTTCCGTGG CCTCGCCCAG GCCGACGGCA
361CCCTCATCAC ATGTGTGGAT TCTGGGATTC TCAGAGTCTG GCATGACAAG GACAAGGACA
421CATCCTCTGA CCCACTCCTG GAACTGAGAG TGGGCCCTGG GGTGTGTAGG ATGCGCCAAG
481ACCCAGCACA CCCCCATGTG GTTGCCACAG GTGGGAAAGA GAATGCTTTG AAGATATGGG
541ACCTGCAGGG CTCTGAGGAA CCTGTTTTCA GGGCCAAGAA CGTGCGGAAT GACTGGCTGG
601ACTTGCGGGT TCCCATCTGG GACCAGGACA TACAGTTTCT CCCAGGATCA CAGAAGCTTG
661TCACCTGCAC AGGGTACCAC CAGGTCCGTG TTTATGATCC AGCATCCCCC CAGCGCCGGC
721CAGTCCTAGA GACCACCTAT GGAGAGTACC CACTAACAGC CATGACCCTC ACTCCGGGAG
781GCAACTCAGT GATTGTGGGA AACACTCATG GGCAGCTGGC AGAAATTGAC CTTCGGCAAG
841GGCGTCTACT GGGCTGTCTG AAGGGGCTGG CAGGCAGTGT GCGTGGGTTG CAGTGCCACC
901CTTCAAAGCC TCTACTAGCC TCCTGTGGCT TGGACAGAGT CTTGAGGATA CACAGGATCC
961AGAATCCACG GGGTCTGGAG CATAAGGATG AGCCCCAAGA GCCTCAAGAA CCCAACAAGG
1021TGCCCCTAGA AGACACAGAG ACAGATGAAC TTTGGGCATC CTTGGAGGCA GCTGCCAAGC
1081GGAAGCTCTC GGGTTTGGAG CAGCCCCAAG GAGCTCTCCA AACGAGACGG AGAAAGAAGA
1141AGCGGCCTGG GTCCACCAGC CCCTGCCCAA CTTTCTTGTA CAAAGTtggc attataagaa
1201agcattgctt atcaatttgt tgcaacgaac

Download FASTA (ORF) (Full)