Transcript: Human NM_001369451.1

Homo sapiens WD repeat domain 74 (WDR74), transcript variant 8, mRNA.

Source:
NCBI, updated 2019-09-10
Taxon:
Homo sapiens (human)
Gene:
WDR74 (54663)
Length:
1737
CDS:
539..1696

Additional Resources:

NCBI RefSeq record:
NM_001369451.1
NBCI Gene record:
WDR74 (54663)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001369451.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000137493 GCAACTCAGTGATTGTGGGAA pLKO.1 1254 CDS 100% 2.640 3.696 N WDR74 n/a
2 TRCN0000424602 CTCCAAACGAGACGGAGAAAG pLKO_005 1646 CDS 100% 10.800 8.640 N WDR74 n/a
3 TRCN0000435387 ACCACCAGGTCCGTGTTTATG pLKO_005 1149 CDS 100% 13.200 9.240 N WDR74 n/a
4 TRCN0000429969 AGCACCGAGGATGGCATATTC pLKO_005 749 CDS 100% 13.200 9.240 N WDR74 n/a
5 TRCN0000135804 CTCATCACATGTGTGGATTCT pLKO.1 836 CDS 100% 4.950 3.465 N WDR74 n/a
6 TRCN0000136220 GACACAGAGACAGATGAACTT pLKO.1 1562 CDS 100% 4.950 3.465 N WDR74 n/a
7 TRCN0000136401 GTGGGAAAGAGAATGCTTTGA pLKO.1 984 CDS 100% 4.950 3.465 N WDR74 n/a
8 TRCN0000137384 GATTCTCAGAGTCTGGCATGA pLKO.1 859 CDS 100% 4.050 2.835 N WDR74 n/a
9 TRCN0000136718 CAGCTGGCAGAAATTGACCTT pLKO.1 1286 CDS 100% 2.640 1.848 N WDR74 n/a
10 TRCN0000134749 GAATGCTTTGAAGATATGGGA pLKO.1 994 CDS 100% 0.750 0.525 N WDR74 n/a
11 TRCN0000135134 CTAGAAGACACAGAGACAGAT pLKO.1 1556 CDS 100% 4.950 2.970 N WDR74 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001369451.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_12070 pDONR223 100% 94.9% 95% None 501G>T;920_976del n/a
2 ccsbBroad304_12070 pLX_304 0% 94.9% 95% V5 501G>T;920_976del n/a
3 TRCN0000469799 AATAAGCACCCACAGGTGGCTTGC pLX_317 40.6% 94.9% 95% V5 501G>T;920_976del n/a
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