Construct: ORF ccsbBroadEn_12153
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009885.1_s300c1, BRDN0000397713
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ARGLU1 (55082)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55082 | ARGLU1 | arginine and glutamate rich 1 | NM_018011.4 | 76.5% | 66.4% | (many diffs) |
| 2 | human | 55082 | ARGLU1 | arginine and glutamate rich 1 | XR_243043.3 | 22.6% | 1_245del;983A>G;1065_3609del | |
| 3 | mouse | 234023 | Arglu1 | arginine and glutamate rich 1 | NM_176849.3 | 74% | 65.1% | (many diffs) |
| 4 | mouse | 234023 | Arglu1 | arginine and glutamate rich 1 | XM_006508759.3 | 68.6% | 59.8% | (many diffs) |
| 5 | mouse | 234023 | Arglu1 | arginine and glutamate rich 1 | XM_006508760.3 | 59.8% | 50% | (many diffs) |
| 6 | mouse | 102465762 | Mir7654 | microRNA 7654 | NR_106114.1 | 7.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 885
- ORF length:
- 819
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG 61 TTGGCATGGG CCGGTCTCGG AGCCGGAGCT CGTCCCGCTC CAAGCACACC AAGAGCAGCA 121 AGCACAACAA GAAGCGCAGC CGGTCCCGGT CGCGATCCCG GGACAAGGAG CGCGTGCGGA 181 AGCGTTCCAA ATCTCGGGAA AGTAAACGGA ACCGGCGGCG GGAGTCGCGG TCCCGTTCGC 241 GCTCCACCAA CACGGCCGTG TCCCGGCGCG AGCGGGACCG GGAGCGCGCC TCGTCCCCGC 301 CCGACCGCAT CGACATCTTC GGGCGCACGG TGAGCAAGCG CAGCAGCCTG GACGAGAAGC 361 AGAAGCGAGA GGAGGAGGAG AAGAAAGCGG AGTTCGAGCG GCAGCGAAAA ATTCGACAGC 421 AAGAAATAGA AGAAAAACTC ATCGAGGAAG AAACAGCACG AAGAGTAGAA GAATTGGTAG 481 CAAAAAGGGT GGAGGAAGAA CTGGAGAAAA GGAAGGATGA AATTGAACGA GAAGTTCTCC 541 GAAGGGTGGA GGAAGCCAAA CGCATCATGG AAAAGCAGTT GCTCGAAGAA CTCGAGCGAC 601 AGAGACAAGC TGAGCTTGCC GCACAAAAAG CTAGAGAGGT AACGCTCGGT CGTTTGGAAA 661 GTAGAGACAG TCCATGGCAA AACTTTCAGT GTTGGGTTCT GCCTCCTGCT CAGTTCAGAA 721 AGAGATGGAA TACAGACTAT CTAATTCCTT TCTCGTCTAA ACTTAACATT GCTGCGAAAG 781 TTAATTTTTT AGCCTATTCA GAGGTGCTGA CTGATAACTT AAAAGTTGGA AGCTTTTATA 841 AAACATATTC AAGGATACTT TTTGATTTAA TGGAACTGGC TATTTGCCCA ACTTTCTTGT 901 ACAAAGTtgg cattataaga aagcattgct tatcaatttg ttgcaacgaa c