Construct: ORF ccsbBroadEn_12250
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002234.1_s300c1, BRDN0000393383
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LIMS2 (55679)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55679 | LIMS2 | LIM zinc finger domain cont... | NM_001256542.1 | 21.6% | 21.6% | 1_444del |
| 2 | human | 55679 | LIMS2 | LIM zinc finger domain cont... | XM_024452985.1 | 21.6% | 21.6% | 1_444del |
| 3 | human | 55679 | LIMS2 | LIM zinc finger domain cont... | XM_024452986.1 | 21.6% | 21.6% | 1_444del |
| 4 | human | 55679 | LIMS2 | LIM zinc finger domain cont... | XM_006712627.4 | 18.2% | 18.2% | 1_552del |
| 5 | human | 55679 | LIMS2 | LIM zinc finger domain cont... | XM_017004469.1 | 17.2% | 17.2% | 1_591del |
| 6 | human | 55679 | LIMS2 | LIM zinc finger domain cont... | XM_005263710.2 | 15% | 15% | 1_693del |
| 7 | human | 55679 | LIMS2 | LIM zinc finger domain cont... | XM_011511453.1 | 12.6% | 12.6% | 1_849del |
| 8 | human | 55679 | LIMS2 | LIM zinc finger domain cont... | NM_001161404.1 | 12.2% | 12.2% | 1_885del |
| 9 | human | 55679 | LIMS2 | LIM zinc finger domain cont... | XM_024452983.1 | 12.2% | 12.2% | 1_885del |
| 10 | human | 55679 | LIMS2 | LIM zinc finger domain cont... | XM_024452984.1 | 12.2% | 12.2% | 1_885del |
| 11 | human | 55679 | LIMS2 | LIM zinc finger domain cont... | NM_001161403.2 | 12% | 12% | 1_900del |
| 12 | human | 55679 | LIMS2 | LIM zinc finger domain cont... | NM_001136037.3 | 11.2% | 11.2% | 1_966del |
| 13 | human | 55679 | LIMS2 | LIM zinc finger domain cont... | NM_017980.4 | 11.2% | 11.2% | 1_972del |
| 14 | human | 55679 | LIMS2 | LIM zinc finger domain cont... | XR_922961.2 | 8.5% | 1_1064del;1165_1166ins22 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 189
- ORF length:
- 123
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG 61 TTGGCATGAA GCCCGTGTGT AAGAGGTGCT ACGAGAAGTT CCCGCTGGAG CTGAAGAAGC 121 GGCTGAAGAA GCTGTCGGAG CTGACCTCCC GCAAGGCCCA GCCCAAGGCC ACAGACCTCA 181 ACTCTGCCTG CCCAACTTTC TTGTACAAAG Ttggcattat aagaaagcat tgcttatcaa 241 tttgttgcaa cgaac