Transcript: Human NM_001256542.1

Homo sapiens LIM zinc finger domain containing 2 (LIMS2), transcript variant 4, mRNA.

Source:
NCBI, updated 2019-06-07
Taxon:
Homo sapiens (human)
Gene:
LIMS2 (55679)
Length:
1759
CDS:
315..884

Additional Resources:

NCBI RefSeq record:
NM_001256542.1
NBCI Gene record:
LIMS2 (55679)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001256542.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000257031 CTCCAGGGACAGGAGCAAATT pLKO_005 1170 3UTR 100% 13.200 9.240 N LIMS2 n/a
2 TRCN0000257025 ACAACTGCAGCCATGTGATTG pLKO_005 631 CDS 100% 10.800 7.560 N LIMS2 n/a
3 TRCN0000244781 TCACCCTGAAGAACAAGTTTG pLKO_005 727 CDS 100% 10.800 7.560 N LIMS2 n/a
4 TRCN0000180591 CCTCCCTTTCTCTTTCCTCAT pLKO.1 988 3UTR 100% 4.050 2.835 N LIMS2 n/a
5 TRCN0000147626 GAAGAACAAGTTTGTGGAGTT pLKO.1 734 CDS 100% 4.050 2.835 N LIMS2 n/a
6 TRCN0000112647 CCCGTGTGTAAGAGGTGCTAT pLKO.1 765 CDS 100% 4.950 3.960 N Lims2 n/a
Download CSV

shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001256542.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

Download CSV

All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_06524 pDONR223 100% 42.7% 46.4% None (many diffs) n/a
2 ccsbBroad304_06524 pLX_304 0% 42.7% 46.4% V5 (many diffs) n/a
3 TRCN0000468941 TAGATCCTATTATCCCACTCTTGT pLX_317 39.8% 42.7% 46.4% V5 (many diffs) n/a
4 ccsbBroadEn_12250 pDONR223 100% 21.6% 21.6% None 1_444del n/a
5 ccsbBroad304_12250 pLX_304 0% 21.6% 21.6% V5 1_444del n/a
6 TRCN0000480198 CAGTAAACTCCATAGGACTTAACC pLX_317 100% 21.6% 21.6% V5 1_444del n/a
Download CSV