Construct: ORF ccsbBroadEn_12499

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF004221.1_s300c1, BRDN0000457068
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
SPATA20 (64847)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 64847 SPATA20 spermatogenesis associated 20 NM_001258373.1 99.8% 99.5% (many diffs)
2 human 64847 SPATA20 spermatogenesis associated 20 NM_001258372.1 94.2% 94% (many diffs)
3 human 64847 SPATA20 spermatogenesis associated 20 NM_022827.4 92.3% 92.1% (many diffs)
4 mouse 217116 Spata20 spermatogenesis associated 20 NM_144827.4 79.3% 83% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2292
ORF length:
2226
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGCC TGCTGGAGGG AAAGGAAGCC ATCCTTCATC TACACCCCAG AGGGTCCCCA
121ACCGCCTGAT CCACGAGAAG TCACCATACC TCCTACAACA TGCCTACAAT CCTGTGGACT
181GGTACCCCTG GGGAGAGGAA GCCTTCGACA AGGCCAGGAA GGAAAACAAG CCGATTTTCC
241TCTCAGTCGG GTACTCCACC TGCCACTGGT GCCACATGAT GGAAGAGGAG TCCTTCCAGA
301ATGAGGAGAT TGGCCGCCTG CTCAGTGAGG ACTTTGTGAG TGTGAAGGTA GACCGTGAGG
361AGCGGCCTGA CGTGGACAAG GTGTACATGA CGTTCGTGCA GGCCACCAGC AGCGGCGGGG
421GCTGGCCCAT GAATGTGTGG CTGACTCCCA ACCTCCAGCC CTTTGTCGGG GGCACCTATT
481TCCCTCCTGA GGATGGCTTG ACCCGAGTCG GCTTCCGCAC AGTGTTGCTG AGAATACGAG
541AACAGTGGAA ACAGAACAAG AACACCCTGC TAGAAAATAG CCAGCGTGTC ACCACTGCCC
601TGCTGGCCCG ATCAGAGATC AGCGTGGGTG ACCGCCAGCT GCCGCCCTCT GCCGCCACCG
661TGAACAATCG CTGCTTCCAG CAGCTGGATG AGGGCTATGA TGAGGAATAC GGTGGCTTCG
721CTGAGGCCCC CAAGTTTCCC ACGCCGGTGA TCCTGAGCTT CCTGTTCTCC TACTGGCTCA
781GCCATCGACT GACTCAGGAT GGCTCTCGGG CCCAGCAGAT GGCCTTGCAT ACCCTGAAAA
841TGATGGCTAA CGGGGGCATC CGGGACCATG TGGGGCAGGG CTTTCACCGC TACTCCACAG
901ACCGCCAGTG GCACGTCCCT CACTTTGAGA AGATGCTCTA TGACCAGGCA CAGCTCGCTG
961TGGCCTATTC GCAGGCCTTC CAGCTCTCTG GTGATGAATT CTACTCTGAC GTGGCCAAAG
1021GCATCCTGCA GTACGTGGCT CGGAGCCTGA GCCACCGGTC CGGAGGCTTC TATAGCGCAG
1081AGGATGCAGA CTCGCCCCCA GAGCGGGGCC AGCGGCCCAA AGAGGGCGCC TACTATGTGT
1141GGACGGTCAA AGAGGTTCAG CAGCTCCTCC CGGAGCCTGT GTTGGGTGCC ACCGAGCCGC
1201TGACCTCAGG CCAGCTCCTC ATGAAGCACT ACGGCCTCAC AGAGGCTGGT AACATCAGCC
1261CCAGTCAGGA CCCCAAGGGG GAGCTGCAGG GCCAGAATGT GCTGACCGTC CGGTACTCGC
1321TGGAGCTGAC TGCTGCCCGC TTTGGCTTGG ATGTGGAGGC CGTGCGGACC TTGCTCAATA
1381CAGGGCTGGA GAAGCTCTTC CAGGCCCGGA AGCATCGGCC CAAGCCGCAC CTGGACAGCA
1441AGATGCTGGC TGCCTGGAAT GGCTTGATGG TGTCAGGCTA TGCTGTGACT GGGGCTGTCC
1501TGGGCCAAGA CAGGCTGATC AACTATGCCA CCAATGGTGC CAAGTTCCTG AAGCGGCACA
1561TGTTTGATGT GGCCAGTGGC CGCCTGATGC GGACCTGCTA CACCGGCCCT GGGGGGACTG
1621TGGAGCACAG CAACCCACCC TGCTGGGGCT TCCTGGAGGA CTACGCCTTC GTGGTGCGGG
1681GCCTGCTGGA CCTGTATGAG GCCTCACAGG AGAGTGCGTG GCTCGAGTGG GCTCTGCGGC
1741TGCAGGACAC ACAGGACAGG CTCTTTTGGG ACTCCCAGGG TGGCGGCTAC TTCTGCAGTG
1801AGGCTGAGCT GGGGGCTGGC CTGCCCCTGC GTCTGAAGGA CGACCAGGAT GGAGCAGAGC
1861CCAGCGCCAA TTCCGTGTCA GCCCACAACC TGCTCCGGCT GCATGGCTTC ACGGGCCACA
1921AGGACTGGAT GGACAAGTGT GTGTGCCTAT TGACCGCCTT TTCCGAGCGC ATGCGTCGTG
1981TCCCGGTGGC GTTGCCCGAG ATGGTCCGCG CCCTCTCAGC CCAGCAGCAG ACCCTCAAGC
2041AGATCGTGAT CTGTGGAGAC CGTCAGGCCA AGGACACCAA GGCCCTGGTG CAGTGCGTCC
2101ACTCTGTCTA CATTCCTAAC AAGGTGCTGA TTCTGGCTGA TGGGGACCCC TCGAGCTTCC
2161TGTCCCGCCA GCTGCCTTTC CTGAGTACCC TCCGACGGTT GGAAGACCAG GCCACTGCAT
2221ATGTGTGTGA GAATCAAGCC TGCTCAGTGC CCATCACTGA TCCCTGCGAA TTACGAAAAC
2281TACTACATCC ATGCCCAACT TTCTTGTACA AAGTTGGcat tataagaaag cattgcttat
2341caatttgttg caacgaac

Download FASTA (ORF) (Full)