Construct: ORF ccsbBroadEn_12754

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF016854.1_s300c1, BRDN0000390316
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
ATG10 (83734)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 83734 ATG10 autophagy related 10 NM_001131028.2 97.8% 85.2% (many diffs)
2 human 83734 ATG10 autophagy related 10 NM_031482.5 97.8% 85.2% (many diffs)
3 human 83734 ATG10 autophagy related 10 XM_005248610.5 97.8% 85.2% (many diffs)
4 human 83734 ATG10 autophagy related 10 XM_005248611.5 97.8% 85.2% (many diffs)
5 human 83734 ATG10 autophagy related 10 XM_011543660.2 82% 81.4% 0_1ins114;509C>T;533C>A
6 human 83734 ATG10 autophagy related 10 XM_005248612.3 81.5% 69.5% (many diffs)
7 human 83734 ATG10 autophagy related 10 XM_017009944.1 81.5% 69.5% (many diffs)
8 human 83734 ATG10 autophagy related 10 XM_011543661.2 68.8% 68% (many diffs)
9 mouse 66795 Atg10 autophagy related 10 XM_011244551.2 68.9% 62.5% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
714
ORF length:
648
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGAG TTCATTGGAG AAAAAACATT CCAACGTTAT TGTGCAGAAT TCATTAAACA
121TTCACAACAT AGGTGATAGT TGGGAATGGA GACCATCAAA GGACTGTTCT GATGGCTACA
181TGTGCAAAAT ACACTTTCAA ATTAAGAATG GGTCTGTGAT GTCACATCTA GGAGCATCTA
241CCCATGGACA GACATGTCTT CCCATGGAGG AGGCTTTCGA GCTACCCTTG GATGATTGTG
301AAGTGATTGA AACTGCAGCA GCGTCCGAAG TGATTAAATA TGAGTATCAT GTCTTATATT
361CCTGTAGCTA CCAAGTGCCT GTACTTTACT TTAGGGCAAG CTTTTTAGAT GGGAGACCTT
421TAACTCTGAA GGACATATGG GAAGGAGTTC ATGAGTGCTA TAAGATGCGA CTGCTACAGG
481GACCATGGGA CACTATTACG CAACAGGAAC ATCCAATACT TGGGCAACCC TTTTTTGTAC
541TTCATCCCTG CAAGACGAAT GAATTCATGA CTCCTGTATT AAAGAATTCT CAGAAAATCA
601ATAAGAATGT CAACTATATC ACATCATGGC TGAGCATTGT AGGGCCAGTT GTTGGGCTGA
661ATCTACCTCT GAGTTATGCC AAAGCAATGT CTCAGGATGA ACGAAATGTC CATTACCCAA
721CTTTCTTGTA CAAAGTtggc attataagaa agcattgctt atcaatttgt tgcaacgaac

Download FASTA (ORF) (Full)