Transcript: Human XM_005248612.3

PREDICTED: Homo sapiens autophagy related 10 (ATG10), transcript variant X3, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
ATG10 (83734)
Length:
5292
CDS:
299..853

Additional Resources:

NCBI RefSeq record:
XM_005248612.3
NBCI Gene record:
ATG10 (83734)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_005248612.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000127548 CAGCGTCCGAAGTGATTAAAT pLKO.1 456 CDS 100% 15.000 21.000 N ATG10 n/a
2 TRCN0000127872 GCTGAATCTACCTCTGAGTTA pLKO.1 793 CDS 100% 4.950 6.930 N ATG10 n/a
3 TRCN0000128633 CCTTTAACTCTGAAGGACATA pLKO.1 554 CDS 100% 4.950 3.960 N ATG10 n/a
4 TRCN0000323068 CCTTTAACTCTGAAGGACATA pLKO_005 554 CDS 100% 4.950 3.960 N ATG10 n/a
5 TRCN0000322995 AGGAGTTCATGAGTGCTATAA pLKO_005 580 CDS 100% 13.200 9.240 N ATG10 n/a
6 TRCN0000322994 ACGTCTCAGGATGAACGAAAT pLKO_005 824 CDS 100% 10.800 7.560 N ATG10 n/a
7 TRCN0000129825 CACAACAGATAGGTGATAGTT pLKO.1 366 CDS 100% 5.625 3.938 N ATG10 n/a
8 TRCN0000127924 GCCAAGTACTTCACTTCCATT pLKO.1 1466 3UTR 100% 4.950 3.465 N ATG10 n/a
9 TRCN0000323069 GCCAAGTACTTCACTTCCATT pLKO_005 1466 3UTR 100% 4.950 3.465 N ATG10 n/a
10 TRCN0000179120 CAACATGGTGAAACCCTGTTT pLKO.1 1159 3UTR 100% 4.950 2.475 Y LOC339059 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_005248612.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_04294 pDONR223 100% 83.6% 83.6% None 106_107ins108 n/a
2 ccsbBroad304_04294 pLX_304 0% 83.6% 83.6% V5 106_107ins108 n/a
3 TRCN0000468766 TCTCAATACCCAATGCCCTTTTTG pLX_317 68.8% 83.6% 83.6% V5 106_107ins108 n/a
4 ccsbBroadEn_12754 pDONR223 100% 81.5% 69.5% None (many diffs) n/a
5 ccsbBroad304_12754 pLX_304 0% 81.5% 69.5% V5 (many diffs) n/a
6 TRCN0000481477 CGGTGAATTACTTTTATCTATGGA pLX_317 67.5% 81.5% 69.5% V5 (many diffs) n/a
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