Construct: ORF ccsbBroadEn_13024
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016384.1_s300c1, BRDN0000392245
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HELQ (113510)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 113510 | HELQ | helicase, POLQ like | NM_001297759.1 | 77% | 76.4% | 807delA;818_1059del |
| 2 | human | 113510 | HELQ | helicase, POLQ like | NM_001297758.1 | 66.5% | 66% | 184_185ins111;696delA;707_948del |
| 3 | human | 113510 | HELQ | helicase, POLQ like | NR_123737.1 | 30.1% | (many diffs) | |
| 4 | human | 113510 | HELQ | helicase, POLQ like | NM_001297755.1 | 26.3% | 26.1% | 807delA;818_3102del |
| 5 | human | 113510 | HELQ | helicase, POLQ like | NM_133636.4 | 24.7% | 24.5% | 807delA;818_3303del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 882
- ORF length:
- 816
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG 61 TTGGCATGGA TGAATGTGGT TCCCGCATCC GCCGGCGGGT GTCTCTCCCC AAAAGGAACC 121 GTCCAAGCTT GGGGTGTATT TTTGGCGCTC CCACCGCGGC CGAGCTCGAG CCCGGAGATG 181 AGGGGAAAGA GGAGGAGGAA ATGGTGGCTG AGAACAGGAG GCGGAAAACC GCGGGCGTAC 241 TGCCGGTTGA GGTACAGCCC CTTCTACTCT CAGATTCCCC GGAATGTCTC GTCCTTGGAG 301 GTGGTGATAC AAACCCGGAC CTCCTACGTC ACATGCCCAC TGACAGAGGG GTGGGAGACC 361 AGCCTAATGA CAGTGAAGTG GACATGTTTG GTGACTATGA TAGCTTTACT GAAAACTCCT 421 TTATAGCTCA AGTTGACGAC CTGGAACAAA AATATATGCA ACTCCCTGAA CATAAGAAAC 481 ATGCTACAGA CTTTGCCACT GAAAATCTTT GCTCGGAAAG TATCAAAAAC AAACTCAGCA 541 TTACTACCAT AGGCAACCTT ACTGAATTAC AAACTGATAA GCACACAGAG AACCAGAGTG 601 GATATGAAGG TGTCACTATT GAACCTGGAG CTGATCTTTT GTATGATGTA CCTTCCTCAC 661 AGGCTATATA CTTTGAAAAT TTGCAGAACT CTTCAAATGA TTTGGGTGAT CATTCTATGA 721 AAGAAAGGGA TTGGAAGTCA TCCTCTCACA ACACTGTGAA TGAGGAACTG CCCCATAATT 781 GCATAGAGCA ACCCCAGCAA AATGATGAGT CCTCTTCCAA AGTCAGAACT AGTTCAGATA 841 TGAACAGGAG AAAAAGTATT AAAGATCATC TAAAAATGCC ATGCCCAACT TTCTGATACA 901 AAGTtggcat tataagaaag cattgcttat caatttgttg caacgaac