Construct: ORF ccsbBroadEn_13119
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009813.1_s300c1, BRDN0000456137
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KDF1 (126695)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 126695 | KDF1 | keratinocyte differentiatio... | NM_152365.3 | 61.3% | 61.3% | 1_462del |
| 2 | human | 126695 | KDF1 | keratinocyte differentiatio... | XM_005245735.2 | 61.3% | 61.3% | 1_462del |
| 3 | human | 126695 | KDF1 | keratinocyte differentiatio... | XM_011540622.2 | 61.3% | 61.3% | 1_462del |
| 4 | mouse | 69073 | Kdf1 | keratinocyte differentiatio... | NM_001083916.1 | 53.9% | 57.5% | (many diffs) |
| 5 | mouse | 69073 | Kdf1 | keratinocyte differentiatio... | NM_133707.2 | 53.9% | 57.5% | (many diffs) |
| 6 | mouse | 69073 | Kdf1 | keratinocyte differentiatio... | XM_006539150.3 | 53.9% | 57.5% | (many diffs) |
| 7 | mouse | 69073 | Kdf1 | keratinocyte differentiatio... | XM_006539151.3 | 53.9% | 57.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 798
- ORF length:
- 732
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG 61 TTGGCATGGG CAGCAGCTTC AGCTACCCCG ATGTTAAGCT CAAAGGCATC CCTGTGTATC 121 CCTACCCGAG GGCCACCTCC CCAGCCCCTG ATGCGGACTC CTGCTGCAAG GAGCCACTGG 181 CCGATCCCCC ACCCATGCGA CACAGCCTGC CCAGCACCTT TGCCAGTAGT CCTCGTGGCT 241 CCGAGGAGTA CTATTCTTTC CATGAGTCGG ACCTGGACCT GCCGGAGATG GGCAGTGGCT 301 CCATGTCGAG CCGAGAAATT GATGTGCTCA TCTTCAAGAA GCTGACAGAG CTGTTCAGCG 361 TACACCAGAT CGATGAGCTG GCCAAGTGCA CATCAGACAC TGTGTTCCTG GAGAAGACCA 421 GTAAGATCTC GGACCTTATC AGCAGCATCA CGCAGGACTA CCACCTGGAT GAGCAGGATG 481 CTGAGGGCCG CCTGGTACGC GGCATCATTC GCATTAGTAC CCGAAAGAGC CGTGCTCGCC 541 CACAGACCTC GGAGGGTCGT TCAACTCGGG CTGCTGCCCC AACCGCTGCT GCCCCTGACA 601 GTGGCCATGA GACCATGGTG GGCTCAGGTC TCAGCCAGGA TGAGCTGACA GTGCAGATCT 661 CCCAGGAGAC GACTGCAGAT GCCATCGCCC GGAAGCTGAG GCCTTATGGA GCTCCAGGGT 721 ACCCAGCAAG CCATGACTCA TCCTTCCAGG GCACCGACAC AGACTCGTCG GGGGCACCCT 781 TGCTCCAGGT GTACTGCTAC CCAACTTTCT TGTACAAAGT TGGcattata agaaagcatt 841 gcttatcaat ttgttgcaac gaac