Construct: ORF ccsbBroadEn_13284
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010393.1_s300c1, BRDN0000392036
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ROPN1B (152015)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 152015 | ROPN1B | rhophilin associated tail p... | XM_005247138.3 | 100% | 100% | |
| 2 | human | 54763 | ROPN1 | rhophilin associated tail p... | NM_001317775.1 | 96.6% | 95% | (many diffs) |
| 3 | human | 152015 | ROPN1B | rhophilin associated tail p... | NM_001012337.3 | 56.6% | 56.6% | 1_276del |
| 4 | human | 152015 | ROPN1B | rhophilin associated tail p... | NM_001308313.2 | 56.6% | 56.6% | 1_276del |
| 5 | human | 152015 | ROPN1B | rhophilin associated tail p... | XM_006713513.3 | 56.6% | 56.6% | 1_276del |
| 6 | human | 54763 | ROPN1 | rhophilin associated tail p... | NM_001317774.1 | 54.7% | 53.7% | (many diffs) |
| 7 | human | 54763 | ROPN1 | rhophilin associated tail p... | NM_017578.4 | 54.7% | 53.7% | (many diffs) |
| 8 | human | 54763 | ROPN1 | rhophilin associated tail p... | XM_011512933.2 | 54.7% | 53.7% | (many diffs) |
| 9 | human | 54763 | ROPN1 | rhophilin associated tail p... | XM_011512934.2 | 54.7% | 53.7% | (many diffs) |
| 10 | mouse | 76378 | Ropn1 | ropporin, rhophilin associa... | NM_030744.2 | 49.6% | 51.8% | (many diffs) |
| 11 | mouse | 76378 | Ropn1 | ropporin, rhophilin associa... | XM_006522698.3 | 49.6% | 51.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 426
- ORF length:
- 360
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG 61 TTGGCATGTG GAAAGTGGTG AATCTCCCAA CAGATCTGTT TAATAGTGTG ATGAATGTGG 121 GTCGCTTCAC GGAGGAGATC GAGTGGCTGA AGTTTTTAGC CCTTGCTTGC AGCGCTCTGG 181 GAGTTACTAT TACCAAAACT CTCAAGATAG TGTGTGAGGT CTTATCATGT GACCACAATG 241 GTGGGTTGCC CCGAATCCCA TTCAGCACCT TCCAGTTTCT CTACACGTAT ATTGCCGAAG 301 TGGATGGGGA GATCTGTGCA TCACATGTCA GCAGGATGCT AAACTACATT GAACAGGAAG 361 TAATTGGTCC TGATGGTTTA ATCACGGTGA ATGACTTTAC CCAAAACCCC AGGGTTTGGC 421 TGGAGTACCC AACTTTCTTG TACAAAGTtg gcattataag aaagcattgc ttatcaattt 481 gttgcaacga ac