Construct: ORF ccsbBroadEn_13301
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016853.1_s300c1, BRDN0000386743
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TMEM65 (157378)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 157378 | TMEM65 | transmembrane protein 65 | NM_194291.2 | 74.1% | 74.1% | 1_186del |
| 2 | mouse | 74868 | Tmem65 | transmembrane protein 65 | NM_175212.4 | 70.2% | 72.2% | (many diffs) |
| 3 | mouse | 74868 | Tmem65 | transmembrane protein 65 | XM_006521504.3 | 62.9% | 64.7% | (many diffs) |
| 4 | mouse | 74868 | Tmem65 | transmembrane protein 65 | XM_011245759.2 | 21.1% | 17.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 600
- ORF length:
- 534
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG 61 TTGGCATGGA GGCGCTGAAC ACGGCGCAGG GCGCGCGCGA CTTCATCTAC AGCCTGCACT 121 CCACGGAGAG GAGCTGCCTG CTCAAAGAGC TGCACCGCTT CGAGTCTATT GCCATTGCCC 181 AAGAAAAATT GGAAGCTCCA CCACCCACCC CAGGACAGCT GAGATATGTA TTCATCCACA 241 ATGCGATACC TTTCATAGGG TTTGGCTTTT TGGATAATGC AATTATGATT GTTGCTGGAA 301 CCCATATTGA AATGTCTATT GGAATTATTT TGGGAATTTC AACTATGGCA GCTGCTGCTT 361 TGGGAAATCT TGTGTCAGAT CTAGCTGGAC TTGGACTTGC AGGCTACGTT GAAGCATTGG 421 CTTCCAGGTT AGGCCTGTCA ATTCCTGATC TCACACCAAA GCAAGTTGAC ATGTGGCAAA 481 CACGTCTTAG TACACATTTG GGCAAAGCTG TTGGGGTGAC TATTGGCTGC ATTCTAGGAA 541 TGTTTCCTTT AATTTTCTTT GGAGGAGGTG AAGAAGATGA AAAACTGGAA ACGAAAAGTT 601 ACCCAACTTT CTTGTACAAA GTtggcatta taagaaagca ttgcttatca atttgttgca 661 acgaac